(Linear) (Six-base) MAPSORT of: KIBB6894.seq  Check: 5866  from: 1  to: 606



KIBB6894.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AvaI C'yCGr_G

Cuts at:      0     359     516     606 
   Size:        359     157      90

  Fragments arranged by size: 

                 359     157      90
 
BanI G'GyrC_C

Cuts at:      0     337     606 
   Size:        337     269
 
BanII G_rGCy'C

Cuts at:      0      70     606 
   Size:         70     536
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     522     606 
   Size:        522      84
 
BbvCI CC'TCA_GC

Cuts at:      0     391     606 
   Size:        391     215
 
BglI GCCn_nnn'nGGC

Cuts at:      0     592     606 
   Size:        592      14
 
Bme1580I G_kGCm'C

Cuts at:      0      29     390     606 
   Size:         29     361     216

  Fragments arranged by size: 

                 361     216      29
 
BmgBI CAC'GTC

Cuts at:      0     495     606 
   Size:        495     111
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     377     606 
   Size:        377     229
 
Bpu10I CC'TnA_GC

Cuts at:      0     391     442     606 
   Size:        391      51     164

  Fragments arranged by size: 

                 391     164      51
 
BsaAI yAC'GTr

Cuts at:      0     480     606 
   Size:        480     126
 
BseYI C'CCAG_C

Cuts at:      0     546     606 
   Size:        546      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     486     606 
   Size:         28     458     120

  Fragments arranged by size: 

                 458     120      28
 
Bsp1286I G_dGCh'C

Cuts at:      0      29      70     390     606 
   Size:         29      41     320     216

  Fragments arranged by size: 

                 320     216      41      29
 
BspHI T'CATG_A

Cuts at:      0     131     606 
   Size:        131     475
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     231     306     606 
   Size:        231      75     300

  Fragments arranged by size: 

                 300     231      75
 
BstYI r'GATC_y

Cuts at:      0     371     606 
   Size:        371     235
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     567     606 
   Size:        567      39
 
BtsI GCAGTG_nn'

Cuts at:      0     177     606 
   Size:        177     429
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     225     260     606 
   Size:        225      35     346

  Fragments arranged by size: 

                 346     225      35
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     546     606 
   Size:        546      60
 
HincII GTy'rAC

Cuts at:      0      90     556     606 
   Size:         90     466      50

  Fragments arranged by size: 

                 466      90      50
 
MslI CAynn'nnrTG

Cuts at:      0     325     586     606 
   Size:        325     261      20

  Fragments arranged by size: 

                 325     261      20
 
PasI CC'CwG_GG

Cuts at:      0     207     345     606 
   Size:        207     138     261

  Fragments arranged by size: 

                 261     207     138
 
PmlI CAC'GTG

Cuts at:      0     480     606 
   Size:        480     126
 
SmaI CCC'GGG

Cuts at:      0     361     606 
   Size:        361     245
 
StuI AGG'CCT

Cuts at:      0     437     601     606 
   Size:        437     164       5

  Fragments arranged by size: 

                 437     164       5
 
StyI C'CwwG_G

Cuts at:      0     333     606 
   Size:        333     273
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     128     606 
   Size:        128     478
 
XmaI C'CCGG_G

Cuts at:      0     359     606 
   Size:        359     247
 

 Enzymes that do cut:

     AvaI     BanI    BanII     BbsI    BbvCI     BglI Bme1580I    BmgBI
     BmrI   Bpu10I    BsaAI    BseYI     BsgI Bsp1286I    BspHI    BstXI
    BstYI    BtgZI     BtsI    CspCI     FalI   HincII     MslI     PasI
     PmlI     SmaI     StuI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI