(Linear) (Six-base) MAPSORT of: KIBB6895.seq  Check: 7855  from: 1  to: 552



KIBB6895.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     176     552 
   Size:        176     376
 
AgeI A'CCGG_T

Cuts at:      0      41     552 
   Size:         41     511
 
AseI AT'TA_AT

Cuts at:      0     233     552 
   Size:        233     319
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     527     552 
   Size:        527      25
 
BclI T'GATC_A

Cuts at:      0     137     383     552 
   Size:        137     246     169

  Fragments arranged by size: 

                 246     169     137
 
BsaBI GATnn'nnATC

Cuts at:      0      28     552 
   Size:         28     524
 
BsaWI w'CCGG_w

Cuts at:      0      41     552 
   Size:         41     511
 
BsmI GAATG_Cn'

Cuts at:      0     429     552 
   Size:        429     123
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     374     552 
   Size:        374     178
 
BsrFI r'CCGG_y

Cuts at:      0      41     552 
   Size:         41     511
 
BstBI TT'CG_AA

Cuts at:      0     480     552 
   Size:        480      72
 
BtgI C'CryG_G

Cuts at:      0     297     552 
   Size:        297     255
 
HindIII A'AGCT_T

Cuts at:      0     404     552 
   Size:        404     148
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     290     552 
   Size:        133     157     262

  Fragments arranged by size: 

                 262     157     133
 
NcoI C'CATG_G

Cuts at:      0     297     552 
   Size:        297     255
 
NdeI CA'TA_TG

Cuts at:      0     371     552 
   Size:        371     181
 
NruI TCG'CGA

Cuts at:      0      28     552 
   Size:         28     524
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      93     552 
   Size:         93     459
 
PfoI T'CCnGG_A

Cuts at:      0      16     552 
   Size:         16     536
 
SspI AAT'ATT

Cuts at:      0     199     552 
   Size:        199     353
 
StyI C'CwwG_G

Cuts at:      0     297     552 
   Size:        297     255
 
TatI w'GTAC_w

Cuts at:      0     327     552 
   Size:        327     225
 

 Enzymes that do cut:

     AccI     AgeI     AseI     BbsI     BclI    BsaBI    BsaWI     BsmI
    BspMI    BsrFI    BstBI     BtgI  HindIII     MmeI     NcoI     NdeI
     NruI    PflMI     PfoI     SspI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI