Restriction map for
pF1KB6896
(Linear) (Six-base) MAPSORT of: KIBB6896.seq Check: 7366 from: 1 to: 606
KIBB6896.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 40 606
Size: 40 566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 535 606
Size: 268 267 71
Fragments arranged by size:
268 267 71
AflIII A'CryG_T
Cuts at: 0 61 475 606
Size: 61 414 131
Fragments arranged by size:
414 131 61
BanII G_rGCy'C
Cuts at: 0 596 606
Size: 596 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 256 478 606
Size: 256 222 128
Fragments arranged by size:
256 222 128
BbvCI CC'TCA_GC
Cuts at: 0 552 606
Size: 552 54
BglI GCCn_nnn'nGGC
Cuts at: 0 337 606
Size: 337 269
BglII A'GATC_T
Cuts at: 0 347 606
Size: 347 259
BlpI GC'TnA_GC
Cuts at: 0 208 606
Size: 208 398
Bme1580I G_kGCm'C
Cuts at: 0 342 606
Size: 342 264
BmgBI CAC'GTC
Cuts at: 0 64 606
Size: 64 542
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 606
Size: 116 490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 513 606
Size: 513 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 295 318 606
Size: 58 237 23 288
Fragments arranged by size:
288 237 58 23
Bpu10I CC'TnA_GC
Cuts at: 0 464 552 606
Size: 464 88 54
Fragments arranged by size:
464 88 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 397 606
Size: 397 209
BsaAI yAC'GTr
Cuts at: 0 478 606
Size: 478 128
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 310 370 606
Size: 280 30 60 236
Fragments arranged by size:
280 236 60 30
BseYI C'CCAG_C
Cuts at: 0 93 177 606
Size: 93 84 429
Fragments arranged by size:
429 93 84
Bsp1286I G_dGCh'C
Cuts at: 0 342 596 606
Size: 342 254 10
Fragments arranged by size:
342 254 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 40 209 437 606
Size: 40 169 228 169
Fragments arranged by size:
228 169 169 40
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 606
Size: 36 570
BstYI r'GATC_y
Cuts at: 0 71 308 347 606
Size: 71 237 39 259
Fragments arranged by size:
259 237 71 39
BtsI GCAGTG_nn'
Cuts at: 0 375 606
Size: 375 231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 435 470 606
Size: 435 35 136
Fragments arranged by size:
435 136 35
EaeI y'GGCC_r
Cuts at: 0 14 606
Size: 14 592
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 268 295 318 535 606
Size: 58 210 27 23 217 71
Fragments arranged by size:
217 210 71 58 27 23
EcoO109I rG'GnC_Cy
Cuts at: 0 375 534 606
Size: 375 159 72
Fragments arranged by size:
375 159 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 606
Size: 580 26
HincII GTy'rAC
Cuts at: 0 390 606
Size: 390 216
MspA1I CmG'CkG
Cuts at: 0 578 606
Size: 578 28
NspI r_CATG'y
Cuts at: 0 452 606
Size: 452 154
PpuMI rG'GwC_Cy
Cuts at: 0 534 606
Size: 534 72
PstI C_TGCA'G
Cuts at: 0 434 583 606
Size: 434 149 23
Fragments arranged by size:
434 149 23
PvuII CAG'CTG
Cuts at: 0 578 606
Size: 578 28
ScaI AGT'ACT
Cuts at: 0 114 606
Size: 114 492
SfcI C'TryA_G
Cuts at: 0 430 579 606
Size: 430 149 27
Fragments arranged by size:
430 149 27
TatI w'GTAC_w
Cuts at: 0 112 606
Size: 112 494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 582 606
Size: 33 549 24
Fragments arranged by size:
549 33 24
Enzymes that do cut:
AarI AcuI AflIII BanII BbsI BbvCI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaAI BseRI BseYI Bsp1286I BspMI BstXI BstYI BtsI
CspCI EaeI Eco57MI EcoO109I Hin4I HincII MspA1I NspI
PpuMI PstI PvuII ScaI SfcI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BcgI BciVI BclI BfrBI BmtI BpuEI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI XmnI ZraI