Restriction map for
pF1KB6902
(Linear) (Six-base) MAPSORT of: KIBB6902.seq Check: 1333 from: 1 to: 669
KIBB6902.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 264 669
Size: 264 405
AatII G_ACGT'C
Cuts at: 0 68 669
Size: 68 601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 553 669
Size: 553 116
AlwNI CAG_nnn'CTG
Cuts at: 0 75 516 669
Size: 75 441 153
Fragments arranged by size:
441 153 75
ApaI G_GGCC'C
Cuts at: 0 394 669
Size: 394 275
AvaI C'yCGr_G
Cuts at: 0 326 669
Size: 326 343
BanI G'GyrC_C
Cuts at: 0 46 669
Size: 46 623
BanII G_rGCy'C
Cuts at: 0 394 669
Size: 394 275
BbeI G_GCGC'C
Cuts at: 0 50 669
Size: 50 619
BbvCI CC'TCA_GC
Cuts at: 0 338 669
Size: 338 331
BclI T'GATC_A
Cuts at: 0 252 669
Size: 252 417
BglI GCCn_nnn'nGGC
Cuts at: 0 143 669
Size: 143 526
Bme1580I G_kGCm'C
Cuts at: 0 394 669
Size: 394 275
BmgBI CAC'GTC
Cuts at: 0 126 198 669
Size: 126 72 471
Fragments arranged by size:
471 126 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 379 669
Size: 379 290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 504 669
Size: 504 165
Bpu10I CC'TnA_GC
Cuts at: 0 338 669
Size: 338 331
BsaHI Gr'CG_yC
Cuts at: 0 47 65 669
Size: 47 18 604
Fragments arranged by size:
604 47 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 106 295 669
Size: 24 82 189 374
Fragments arranged by size:
374 189 82 24
BseYI C'CCAG_C
Cuts at: 0 438 620 639 669
Size: 438 182 19 30
Fragments arranged by size:
438 182 30 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 283 559 669
Size: 274 9 276 110
Fragments arranged by size:
276 274 110 9
Bsp1286I G_dGCh'C
Cuts at: 0 394 669
Size: 394 275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 264 669
Size: 264 405
BsrBI CCG'CTC
Cuts at: 0 26 669
Size: 26 643
BsrFI r'CCGG_y
Cuts at: 0 43 669
Size: 43 626
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 669
Size: 297 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 620 669
Size: 620 49
BtgI C'CryG_G
Cuts at: 0 397 669
Size: 397 272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 432 669
Size: 432 237
BtsI GCAGTG_nn'
Cuts at: 0 269 514 537 669
Size: 269 245 23 132
Fragments arranged by size:
269 245 132 23
EcoO109I rG'GnC_Cy
Cuts at: 0 285 390 391 669
Size: 285 105 1 278
Fragments arranged by size:
285 278 105 1
HaeII r_GCGC'y
Cuts at: 0 50 669
Size: 50 619
KasI G'GCGC_C
Cuts at: 0 46 669
Size: 46 623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 637 669
Size: 29 608 32
Fragments arranged by size:
608 32 29
MslI CAynn'nnrTG
Cuts at: 0 151 669
Size: 151 518
MspA1I CmG'CkG
Cuts at: 0 165 297 669
Size: 165 132 372
Fragments arranged by size:
372 165 132
NaeI GCC'GGC
Cuts at: 0 45 669
Size: 45 624
NarI GG'CG_CC
Cuts at: 0 47 669
Size: 47 622
NdeI CA'TA_TG
Cuts at: 0 238 669
Size: 238 431
NgoMIV G'CCGG_C
Cuts at: 0 43 669
Size: 43 626
NruI TCG'CGA
Cuts at: 0 308 669
Size: 308 361
PfoI T'CCnGG_A
Cuts at: 0 68 669
Size: 68 601
PspOMI G'GGCC_C
Cuts at: 0 390 669
Size: 390 279
PstI C_TGCA'G
Cuts at: 0 248 669
Size: 248 421
PvuII CAG'CTG
Cuts at: 0 297 669
Size: 297 372
SfcI C'TryA_G
Cuts at: 0 244 669
Size: 244 425
SfoI GGC'GCC
Cuts at: 0 48 669
Size: 48 621
SmaI CCC'GGG
Cuts at: 0 328 669
Size: 328 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 514 669
Size: 514 155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 145 669
Size: 145 524
XmaI C'CCGG_G
Cuts at: 0 326 669
Size: 326 343
ZraI GAC'GTC
Cuts at: 0 66 669
Size: 66 603
Enzymes that do cut:
AarI AatII AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbvCI BclI BglI Bme1580I BmgBI BmrI BplI
Bpu10I BsaHI BseRI BseYI BsgI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstXI BtgI BtgZI BtsI EcoO109I HaeII
KasI MmeI MslI MspA1I NaeI NarI NdeI NgoMIV
NruI PfoI PspOMI PstI PvuII SfcI SfoI SmaI
TaqII XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BglII
BlpI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI NcoI NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI