(Linear) (Six-base) MAPSORT of: KIBB6903.seq  Check: 7017  from: 1  to: 609



KIBB6903.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

Acc65I G'GTAC_C

Cuts at:      0     245     609 
   Size:        245     364
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     490     609 
   Size:         70     420     119

  Fragments arranged by size: 

                 420     119      70
 
AfeI AGC'GCT

Cuts at:      0     475     609 
   Size:        475     134
 
AgeI A'CCGG_T

Cuts at:      0     248     609 
   Size:        248     361
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60     609 
   Size:         60     549
 
ApoI r'AATT_y

Cuts at:      0     152     299     609 
   Size:        152     147     310

  Fragments arranged by size: 

                 310     152     147
 
BanI G'GyrC_C

Cuts at:      0     245     609 
   Size:        245     364
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     599     609 
   Size:        599      10
 
BbvCI CC'TCA_GC

Cuts at:      0     335     609 
   Size:        335     274
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     445     544     609 
   Size:        445      99      65

  Fragments arranged by size: 

                 445      99      65
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526     609 
   Size:        526      83
 
Bpu10I CC'TnA_GC

Cuts at:      0     335     609 
   Size:        335     274
 
BsaBI GATnn'nnATC

Cuts at:      0     464     609 
   Size:        464     145
 
BsaWI w'CCGG_w

Cuts at:      0     248     609 
   Size:        248     361
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     355     385     609 
   Size:        355      30     224

  Fragments arranged by size: 

                 355     224      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     376     609 
   Size:         94     282     233

  Fragments arranged by size: 

                 282     233      94
 
BsrBI CCG'CTC

Cuts at:      0      84     609 
   Size:         84     525
 
BsrFI r'CCGG_y

Cuts at:      0     248     609 
   Size:        248     361
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      60     230     609 
   Size:         60     170     379

  Fragments arranged by size: 

                 379     170      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      82     609 
   Size:         82     527
 
EarI CTCTTCn'nnn_

Cuts at:      0     188     609 
   Size:        188     421
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     490     526     609 
   Size:         70     420      36      83

  Fragments arranged by size: 

                 420      83      70      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0     546     609 
   Size:        546      63
 
HaeII r_GCGC'y

Cuts at:      0      26     477     609 
   Size:         26     451     132

  Fragments arranged by size: 

                 451     132      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     212     244     355     374     387     406     609 
   Size:        212      32     111      19      13      19     203

  Fragments arranged by size: 

                 212     203     111      32      19      19      13
 
HincII GTy'rAC

Cuts at:      0     602     609 
   Size:        602       7
 
KpnI G_GTAC'C

Cuts at:      0     249     609 
   Size:        249     360
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     609 
   Size:        240     369
 
MslI CAynn'nnrTG

Cuts at:      0     271     397     609 
   Size:        271     126     212

  Fragments arranged by size: 

                 271     212     126
 
MspA1I CmG'CkG

Cuts at:      0      13     609 
   Size:         13     596
 
NspI r_CATG'y

Cuts at:      0     174     609 
   Size:        174     435
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     409     609 
   Size:        409     200
 
PfoI T'CCnGG_A

Cuts at:      0     322     609 
   Size:        322     287
 
PpuMI rG'GwC_Cy

Cuts at:      0     546     609 
   Size:        546      63
 
SphI G_CATG'C

Cuts at:      0     174     609 
   Size:        174     435
 
StuI AGG'CCT

Cuts at:      0     367     407     609 
   Size:        367      40     202

  Fragments arranged by size: 

                 367     202      40
 
StyI C'CwwG_G

Cuts at:      0     408     609 
   Size:        408     201
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI     AgeI    AlwNI     ApoI     BanI     BbsI
    BbvCI     BmrI     BpmI   Bpu10I    BsaBI    BsaWI    BsaXI    BseRI
    BsrBI    BsrFI   BstAPI    BtgZI     EarI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     KpnI     MmeI     MslI   MspA1I     NspI    PflMI
     PfoI    PpuMI     SphI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII   AflIII     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI