(Linear) (Six-base) MAPSORT of: KIBB6906.seq  Check: 3971  from: 1  to: 669



KIBB6906.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AleI CACnn'nnGTG

Cuts at:      0     658     669 
   Size:        658      11
 
ApoI r'AATT_y

Cuts at:      0     337     366     515     669 
   Size:        337      29     149     154

  Fragments arranged by size: 

                 337     154     149      29
 
BanI G'GyrC_C

Cuts at:      0     455     669 
   Size:        455     214
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     306     669 
   Size:        306     363
 
BfrBI ATG'CAT

Cuts at:      0      75     669 
   Size:         75     594
 
Bme1580I G_kGCm'C

Cuts at:      0     460     669 
   Size:        460     209
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     423     669 
   Size:        423     246
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     486     516     669 
   Size:        486      30     153

  Fragments arranged by size: 

                 486     153      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     570     669 
   Size:        570      99
 
BsiHKAI G_wGCw'C

Cuts at:      0     301     669 
   Size:        301     368
 
Bsp1286I G_dGCh'C

Cuts at:      0     301     460     669 
   Size:        301     159     209

  Fragments arranged by size: 

                 301     209     159
 
BsrGI T'GTAC_A

Cuts at:      0     173     669 
   Size:        173     496
 
BstYI r'GATC_y

Cuts at:      0     109     669 
   Size:        109     560
 
BtsI GCAGTG_nn'

Cuts at:      0     337     669 
   Size:        337     332
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9     669 
   Size:          9     660
 
EaeI y'GGCC_r

Cuts at:      0     180     669 
   Size:        180     489
 
MslI CAynn'nnrTG

Cuts at:      0     658     669 
   Size:        658      11
 
MspA1I CmG'CkG

Cuts at:      0     260     669 
   Size:        260     409
 
NsiI A_TGCA'T

Cuts at:      0      77     669 
   Size:         77     592
 
PasI CC'CwG_GG

Cuts at:      0     270     669 
   Size:        270     399
 
PsiI TTA'TAA

Cuts at:      0     254     669 
   Size:        254     415
 
PvuII CAG'CTG

Cuts at:      0     260     669 
   Size:        260     409
 
ScaI AGT'ACT

Cuts at:      0     193     669 
   Size:        193     476
 
SfcI C'TryA_G

Cuts at:      0     402     669 
   Size:        402     267
 
StyI C'CwwG_G

Cuts at:      0     151     669 
   Size:        151     518
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     648     669 
   Size:        145     503      21

  Fragments arranged by size: 

                 503     145      21
 
TatI w'GTAC_w

Cuts at:      0     173     191     669 
   Size:        173      18     478

  Fragments arranged by size: 

                 478     173      18
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     327     359     475     486     507     518     669 
   Size:        327      32     116      11      21      11     151

  Fragments arranged by size: 

                 327     151     116      32      21      11      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      21     471     669 
   Size:         21     450     198

  Fragments arranged by size: 

                 450     198      21
 

 Enzymes that do cut:

     AleI     ApoI     BanI     BbsI    BfrBI Bme1580I     BsaI    BsaXI
     BsgI  BsiHKAI Bsp1286I    BsrGI    BstYI     BtsI     DrdI     EaeI
     MslI   MspA1I     NsiI     PasI     PsiI    PvuII     ScaI     SfcI
     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI