Restriction map for
pF1KB6907
(Linear) (Six-base) MAPSORT of: KIBB6907.seq Check: 2550 from: 1 to: 612
KIBB6907.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 612
Size: 331 281
AhdI GACnn_n'nnGTC
Cuts at: 0 567 612
Size: 567 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 454 486 612
Size: 454 32 126
Fragments arranged by size:
454 126 32
AlwNI CAG_nnn'CTG
Cuts at: 0 120 612
Size: 120 492
ApaI G_GGCC'C
Cuts at: 0 253 452 612
Size: 253 199 160
Fragments arranged by size:
253 199 160
AvaI C'yCGr_G
Cuts at: 0 246 452 512 612
Size: 246 206 60 100
Fragments arranged by size:
246 206 100 60
BanI G'GyrC_C
Cuts at: 0 492 612
Size: 492 120
BanII G_rGCy'C
Cuts at: 0 17 253 452 612
Size: 17 236 199 160
Fragments arranged by size:
236 199 160 17
BlpI GC'TnA_GC
Cuts at: 0 5 612
Size: 5 607
Bme1580I G_kGCm'C
Cuts at: 0 253 452 612
Size: 253 199 160
Fragments arranged by size:
253 199 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 301 612
Size: 277 24 311
Fragments arranged by size:
311 277 24
BsaAI yAC'GTr
Cuts at: 0 395 612
Size: 395 217
BsaBI GATnn'nnATC
Cuts at: 0 337 612
Size: 337 275
BsaHI Gr'CG_yC
Cuts at: 0 407 612
Size: 407 205
BsaWI w'CCGG_w
Cuts at: 0 329 612
Size: 329 283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 612
Size: 454 30 128
Fragments arranged by size:
454 128 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 475 502 612
Size: 70 405 27 110
Fragments arranged by size:
405 110 70 27
BseYI C'CCAG_C
Cuts at: 0 165 612
Size: 165 447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 612
Size: 255 357
BsiHKAI G_wGCw'C
Cuts at: 0 68 612
Size: 68 544
Bsp1286I G_dGCh'C
Cuts at: 0 17 68 253 452 612
Size: 17 51 185 199 160
Fragments arranged by size:
199 185 160 51 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 612
Size: 210 402
BstYI r'GATC_y
Cuts at: 0 110 612
Size: 110 502
BtgI C'CryG_G
Cuts at: 0 482 612
Size: 482 130
DrdI GACnn_nn'nnGTC
Cuts at: 0 196 612
Size: 196 416
EaeI y'GGCC_r
Cuts at: 0 176 612
Size: 176 436
EarI CTCTTCn'nnn_
Cuts at: 0 91 612
Size: 91 521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 301 331 612
Size: 277 24 30 281
Fragments arranged by size:
281 277 30 24
EcoO109I rG'GnC_Cy
Cuts at: 0 159 250 449 612
Size: 159 91 199 163
Fragments arranged by size:
199 163 159 91
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 555 612
Size: 555 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 352 384 553 585 612
Size: 52 32 268 32 169 32 27
Fragments arranged by size:
268 169 52 32 32 32 27
HincII GTy'rAC
Cuts at: 0 75 354 612
Size: 75 279 258
Fragments arranged by size:
279 258 75
HpaI GTT'AAC
Cuts at: 0 354 612
Size: 354 258
MspA1I CmG'CkG
Cuts at: 0 30 46 612
Size: 30 16 566
Fragments arranged by size:
566 30 16
PflMI CCAn_nnn'nTGG
Cuts at: 0 120 612
Size: 120 492
PfoI T'CCnGG_A
Cuts at: 0 104 113 612
Size: 104 9 499
Fragments arranged by size:
499 104 9
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 612
Size: 454 32 126
Fragments arranged by size:
454 126 32
PspOMI G'GGCC_C
Cuts at: 0 249 448 612
Size: 249 199 164
Fragments arranged by size:
249 199 164
PvuII CAG'CTG
Cuts at: 0 30 612
Size: 30 582
SapI GCTCTTCn'nnn_
Cuts at: 0 91 612
Size: 91 521
SfcI C'TryA_G
Cuts at: 0 522 612
Size: 522 90
SmaI CCC'GGG
Cuts at: 0 248 454 514 612
Size: 248 206 60 98
Fragments arranged by size:
248 206 98 60
SnaBI TAC'GTA
Cuts at: 0 395 612
Size: 395 217
StyI C'CwwG_G
Cuts at: 0 497 612
Size: 497 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 60 510 574 612
Size: 60 450 64 38
Fragments arranged by size:
450 64 60 38
TatI w'GTAC_w
Cuts at: 0 149 380 612
Size: 149 231 232
Fragments arranged by size:
232 231 149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 612
Size: 504 108
XmaI C'CCGG_G
Cuts at: 0 246 452 512 612
Size: 246 206 60 100
Fragments arranged by size:
246 206 100 60
Enzymes that do cut:
AcuI AhdI AloI AlwNI ApaI AvaI BanI BanII
BlpI Bme1580I BpmI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BstXI BstYI BtgI
DrdI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII
HpaI MspA1I PflMI PfoI PpiI PspOMI PvuII SapI
SfcI SmaI SnaBI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmnI ZraI