Restriction map for
pF1KB6909
(Linear) (Six-base) MAPSORT of: KIBB6909.seq Check: 9841 from: 1 to: 615
KIBB6909.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
Acc65I G'GTAC_C
Cuts at: 0 65 615
Size: 65 550
AlwNI CAG_nnn'CTG
Cuts at: 0 149 586 615
Size: 149 437 29
Fragments arranged by size:
437 149 29
ApaI G_GGCC'C
Cuts at: 0 40 567 580 615
Size: 40 527 13 35
Fragments arranged by size:
527 40 35 13
BanI G'GyrC_C
Cuts at: 0 65 615
Size: 65 550
BanII G_rGCy'C
Cuts at: 0 40 196 567 580 615
Size: 40 156 371 13 35
Fragments arranged by size:
371 156 40 35 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 615
Size: 586 29
BlpI GC'TnA_GC
Cuts at: 0 229 615
Size: 229 386
Bme1580I G_kGCm'C
Cuts at: 0 40 484 567 580 615
Size: 40 444 83 13 35
Fragments arranged by size:
444 83 40 35 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 538 615
Size: 538 77
BmtI G_CTAG'C
Cuts at: 0 517 615
Size: 517 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 258 615
Size: 258 357
BsaAI yAC'GTr
Cuts at: 0 162 615
Size: 162 453
BsaBI GATnn'nnATC
Cuts at: 0 538 615
Size: 538 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 615
Size: 109 30 476
Fragments arranged by size:
476 109 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 388 453 615
Size: 133 255 65 162
Fragments arranged by size:
255 162 133 65
BseYI C'CCAG_C
Cuts at: 0 40 44 566 615
Size: 40 4 522 49
Fragments arranged by size:
522 49 40 4
Bsp1286I G_dGCh'C
Cuts at: 0 40 196 484 567 580 615
Size: 40 156 288 83 13 35
Fragments arranged by size:
288 156 83 40 35 13
BsrBI CCG'CTC
Cuts at: 0 378 615
Size: 378 237
BsrFI r'CCGG_y
Cuts at: 0 233 361 615
Size: 233 128 254
Fragments arranged by size:
254 233 128
BssHII G'CGCG_C
Cuts at: 0 224 615
Size: 224 391
BssSI C'ACGA_G
Cuts at: 0 370 615
Size: 370 245
BstYI r'GATC_y
Cuts at: 0 260 615
Size: 260 355
Bsu36I CC'TnA_GG
Cuts at: 0 476 615
Size: 476 139
BtgI C'CryG_G
Cuts at: 0 491 615
Size: 491 124
BtsI GCAGTG_nn'
Cuts at: 0 136 206 269 615
Size: 136 70 63 346
Fragments arranged by size:
346 136 70 63
EaeI y'GGCC_r
Cuts at: 0 151 203 615
Size: 151 52 412
Fragments arranged by size:
412 151 52
EarI CTCTTCn'nnn_
Cuts at: 0 88 615
Size: 88 527
EcoO109I rG'GnC_Cy
Cuts at: 0 36 37 48 498 576 615
Size: 36 1 11 450 78 39
Fragments arranged by size:
450 78 39 36 11 1
HaeII r_GCGC'y
Cuts at: 0 12 615
Size: 12 603
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 615
Size: 109 32 474
Fragments arranged by size:
474 109 32
HincII GTy'rAC
Cuts at: 0 303 615
Size: 303 312
KpnI G_GTAC'C
Cuts at: 0 69 615
Size: 69 546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 615
Size: 551 64
MscI TGG'CCA
Cuts at: 0 153 615
Size: 153 462
MspA1I CmG'CkG
Cuts at: 0 44 149 248 281 615
Size: 44 105 99 33 334
Fragments arranged by size:
334 105 99 44 33
NaeI GCC'GGC
Cuts at: 0 235 615
Size: 235 380
NgoMIV G'CCGG_C
Cuts at: 0 233 615
Size: 233 382
NheI G'CTAG_C
Cuts at: 0 513 615
Size: 513 102
PpuMI rG'GwC_Cy
Cuts at: 0 48 615
Size: 48 567
PspOMI G'GGCC_C
Cuts at: 0 36 563 576 615
Size: 36 527 13 39
Fragments arranged by size:
527 39 36 13
PvuII CAG'CTG
Cuts at: 0 44 149 615
Size: 44 105 466
Fragments arranged by size:
466 105 44
RsrII CG'GwC_CG
Cuts at: 0 276 615
Size: 276 339
SanDI GG'GwC_CC
Cuts at: 0 48 615
Size: 48 567
SfcI C'TryA_G
Cuts at: 0 216 615
Size: 216 399
StuI AGG'CCT
Cuts at: 0 94 615
Size: 94 521
StyI C'CwwG_G
Cuts at: 0 338 615
Size: 338 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 466 615
Size: 19 447 149
Fragments arranged by size:
447 149 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 615
Size: 345 270
Enzymes that do cut:
Acc65I AlwNI ApaI BanI BanII BcgI BlpI Bme1580I
BmrI BmtI BsaI BsaAI BsaBI BsaXI BseRI BseYI
Bsp1286I BsrBI BsrFI BssHII BssSI BstYI Bsu36I BtgI
BtsI EaeI EarI EcoO109I HaeII Hin4I HincII KpnI
MmeI MscI MspA1I NaeI NgoMIV NheI PpuMI PspOMI
PvuII RsrII SanDI SfcI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BmgBI BplI
BpmI Bpu10I BpuEI BsaHI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI MfeI MluI MslI NarI NcoI NdeI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI