Restriction map for
pF1KB6911
(Linear) (Six-base) MAPSORT of: KIBB6911.seq Check: 3528 from: 1 to: 615
KIBB6911.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 430 615
Size: 430 185
AatII G_ACGT'C
Cuts at: 0 179 615
Size: 179 436
BamHI G'GATC_C
Cuts at: 0 86 474 615
Size: 86 388 141
Fragments arranged by size:
388 141 86
BanI G'GyrC_C
Cuts at: 0 418 615
Size: 418 197
BanII G_rGCy'C
Cuts at: 0 167 467 615
Size: 167 300 148
Fragments arranged by size:
300 167 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 349 615
Size: 349 266
BglI GCCn_nnn'nGGC
Cuts at: 0 34 615
Size: 34 581
BglII A'GATC_T
Cuts at: 0 122 615
Size: 122 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 615
Size: 184 431
BsaBI GATnn'nnATC
Cuts at: 0 479 615
Size: 479 136
BsaHI Gr'CG_yC
Cuts at: 0 176 615
Size: 176 439
BsaWI w'CCGG_w
Cuts at: 0 477 615
Size: 477 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 44 542 572 615
Size: 14 30 498 30 43
Fragments arranged by size:
498 43 30 30 14
BseYI C'CCAG_C
Cuts at: 0 191 615
Size: 191 424
BsmI GAATG_Cn'
Cuts at: 0 522 615
Size: 522 93
Bsp1286I G_dGCh'C
Cuts at: 0 167 467 615
Size: 167 300 148
Fragments arranged by size:
300 167 148
BspEI T'CCGG_A
Cuts at: 0 477 615
Size: 477 138
BspHI T'CATG_A
Cuts at: 0 260 615
Size: 260 355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 430 615
Size: 430 185
BsrDI GCAATG_nn'
Cuts at: 0 323 615
Size: 323 292
BsrFI r'CCGG_y
Cuts at: 0 140 615
Size: 140 475
BsrGI T'GTAC_A
Cuts at: 0 146 615
Size: 146 469
BstEII G'GTnAC_C
Cuts at: 0 102 136 615
Size: 102 34 479
Fragments arranged by size:
479 102 34
BstYI r'GATC_y
Cuts at: 0 86 122 474 615
Size: 86 36 352 141
Fragments arranged by size:
352 141 86 36
BtgI C'CryG_G
Cuts at: 0 107 131 389 615
Size: 107 24 258 226
Fragments arranged by size:
258 226 107 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 48 615
Size: 48 567
BtsI GCAGTG_nn'
Cuts at: 0 542 615
Size: 542 73
DrdI GACnn_nn'nnGTC
Cuts at: 0 409 615
Size: 409 206
EaeI y'GGCC_r
Cuts at: 0 35 59 158 272 615
Size: 35 24 99 114 343
Fragments arranged by size:
343 114 99 35 24
HaeII r_GCGC'y
Cuts at: 0 81 198 615
Size: 81 117 417
Fragments arranged by size:
417 117 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 523 542 555 574 615
Size: 14 32 477 19 13 19 41
Fragments arranged by size:
477 41 32 19 19 14 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 615
Size: 314 301
MscI TGG'CCA
Cuts at: 0 37 61 274 615
Size: 37 24 213 341
Fragments arranged by size:
341 213 37 24
MslI CAynn'nnrTG
Cuts at: 0 37 265 615
Size: 37 228 350
Fragments arranged by size:
350 228 37
NcoI C'CATG_G
Cuts at: 0 131 389 615
Size: 131 258 226
Fragments arranged by size:
258 226 131
PstI C_TGCA'G
Cuts at: 0 72 615
Size: 72 543
SfcI C'TryA_G
Cuts at: 0 68 615
Size: 68 547
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 34 615
Size: 34 581
SmlI C'TyrA_G
Cuts at: 0 199 615
Size: 199 416
StyI C'CwwG_G
Cuts at: 0 131 389 615
Size: 131 258 226
Fragments arranged by size:
258 226 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 142 615
Size: 124 18 473
Fragments arranged by size:
473 124 18
TatI w'GTAC_w
Cuts at: 0 146 615
Size: 146 469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 39 71 615
Size: 39 32 544
Fragments arranged by size:
544 39 32
XbaI T'CTAG_A
Cuts at: 0 369 615
Size: 369 246
ZraI GAC'GTC
Cuts at: 0 177 615
Size: 177 438
Enzymes that do cut:
AarI AatII BamHI BanI BanII BbsI BglI BglII
BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BsrGI BstEII BstYI
BtgI BtgZI BtsI DrdI EaeI HaeII Hin4I MmeI
MscI MslI NcoI PstI SfcI SfiI SmlI StyI
TaqII TatI TstI XbaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaI BsaAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsrBI BssHII BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XcmI XhoI XmaI XmnI