(Linear) (Six-base) MAPSORT of: KIBB6913.seq  Check: 5089  from: 1  to: 615



KIBB6913.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

ApoI r'AATT_y

Cuts at:      0     349     615 
   Size:        349     266
 
BglII A'GATC_T

Cuts at:      0     338     615 
   Size:        338     277
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     490     615 
   Size:         57     433     125

  Fragments arranged by size: 

                 433     125      57
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      54     271     615 
   Size:         54     217     344

  Fragments arranged by size: 

                 344     217      54
 
BspHI T'CATG_A

Cuts at:      0     204     615 
   Size:        204     411
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       7     448     615 
   Size:          7     441     167

  Fragments arranged by size: 

                 441     167       7
 
BsrFI r'CCGG_y

Cuts at:      0      24     615 
   Size:         24     591
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     589     615 
   Size:        589      26
 
BstYI r'GATC_y

Cuts at:      0     338     615 
   Size:        338     277
 
EarI CTCTTCn'nnn_

Cuts at:      0     281     398     615 
   Size:        281     117     217

  Fragments arranged by size: 

                 281     217     117
 
EcoRI G'AATT_C

Cuts at:      0     349     615 
   Size:        349     266
 
HincII GTy'rAC

Cuts at:      0     534     615 
   Size:        534      81
 
HindIII A'AGCT_T

Cuts at:      0     230     615 
   Size:        230     385
 
MslI CAynn'nnrTG

Cuts at:      0     209     615 
   Size:        209     406
 
MspA1I CmG'CkG

Cuts at:      0     281     545     615 
   Size:        281     264      70

  Fragments arranged by size: 

                 281     264      70
 
NaeI GCC'GGC

Cuts at:      0      26     615 
   Size:         26     589
 
NgoMIV G'CCGG_C

Cuts at:      0      24     615 
   Size:         24     591
 
NspI r_CATG'y

Cuts at:      0     423     615 
   Size:        423     192
 
PvuII CAG'CTG

Cuts at:      0     281     545     615 
   Size:        281     264      70

  Fragments arranged by size: 

                 281     264      70
 
SmlI C'TyrA_G

Cuts at:      0      36     505     615 
   Size:         36     469     110

  Fragments arranged by size: 

                 469     110      36
 
SspI AAT'ATT

Cuts at:      0     264     615 
   Size:        264     351
 
StyI C'CwwG_G

Cuts at:      0     326     615 
   Size:        326     289
 
TatI w'GTAC_w

Cuts at:      0     290     615 
   Size:        290     325
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      94     126     267     299     615 
   Size:         94      32     141      32     316

  Fragments arranged by size: 

                 316     141      94      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     466     615 
   Size:        466     149
 

 Enzymes that do cut:

     ApoI    BglII    BpuEI    BseRI    BspHI    BspMI    BsrFI   BstAPI
    BstYI     EarI    EcoRI   HincII  HindIII     MslI   MspA1I     NaeI
   NgoMIV     NspI    PvuII     SmlI     SspI     StyI     TatI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI