(Linear) (Six-base) MAPSORT of: KIBB6915.seq  Check: 9826  from: 1  to: 615



KIBB6915.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

Acc65I G'GTAC_C

Cuts at:      0     309     615 
   Size:        309     306
 
AflII C'TTAA_G

Cuts at:      0     319     615 
   Size:        319     296
 
ApaI G_GGCC'C

Cuts at:      0      14     615 
   Size:         14     601
 
AseI AT'TA_AT

Cuts at:      0     352     615 
   Size:        352     263
 
AvrII C'CTAG_G

Cuts at:      0     174     615 
   Size:        174     441
 
BanI G'GyrC_C

Cuts at:      0     309     615 
   Size:        309     306
 
BanII G_rGCy'C

Cuts at:      0      14     615 
   Size:         14     601
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     117     209     240     520     615 
   Size:        117      92      31     280      95

  Fragments arranged by size: 

                 280     117      95      92      31
 
Bme1580I G_kGCm'C

Cuts at:      0      14     119     615 
   Size:         14     105     496

  Fragments arranged by size: 

                 496     105      14
 
BmgBI CAC'GTC

Cuts at:      0      68     615 
   Size:         68     547
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      13     615 
   Size:         13     602
 
BsaAI yAC'GTr

Cuts at:      0     114     615 
   Size:        114     501
 
BsaHI Gr'CG_yC

Cuts at:      0      95     615 
   Size:         95     520
 
BsaWI w'CCGG_w

Cuts at:      0     312     615 
   Size:        312     303
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     316     615 
   Size:        121     195     299

  Fragments arranged by size: 

                 299     195     121
 
BsiEI CG_ry'CG

Cuts at:      0     194     615 
   Size:        194     421
 
Bsp1286I G_dGCh'C

Cuts at:      0      14     119     615 
   Size:         14     105     496

  Fragments arranged by size: 

                 496     105      14
 
BspHI T'CATG_A

Cuts at:      0      77     615 
   Size:         77     538
 
BssHII G'CGCG_C

Cuts at:      0      17     615 
   Size:         17     598
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      68     615 
   Size:         68     547
 
BtgI C'CryG_G

Cuts at:      0     152     615 
   Size:        152     463
 
EaeI y'GGCC_r

Cuts at:      0     191     615 
   Size:        191     424
 
EagI C'GGCC_G

Cuts at:      0     191     615 
   Size:        191     424
 
KpnI G_GTAC'C

Cuts at:      0     313     615 
   Size:        313     302
 
NspI r_CATG'y

Cuts at:      0     473     615 
   Size:        473     142
 
PfoI T'CCnGG_A

Cuts at:      0     101     615 
   Size:        101     514
 
PshAI GACnn'nnGTC

Cuts at:      0     244     615 
   Size:        244     371
 
PspOMI G'GGCC_C

Cuts at:      0      10     615 
   Size:         10     605
 
SmlI C'TyrA_G

Cuts at:      0      28     319     615 
   Size:         28     291     296

  Fragments arranged by size: 

                 296     291      28
 
SphI G_CATG'C

Cuts at:      0     473     615 
   Size:        473     142
 
StuI AGG'CCT

Cuts at:      0     222     615 
   Size:        222     393
 
StyI C'CwwG_G

Cuts at:      0     174     615 
   Size:        174     441
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     256     615 
   Size:        256     359
 

 Enzymes that do cut:

   Acc65I    AflII     ApaI     AseI    AvrII     BanI    BanII     BbsI
 Bme1580I    BmgBI    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BsiEI
 Bsp1286I    BspHI   BssHII   BstAPI     BtgI     EaeI     EagI     KpnI
     NspI     PfoI    PshAI   PspOMI     SmlI     SphI     StuI     StyI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
    AsiSI     AvaI     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaBI    BsaXI    BseYI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI