Restriction map for
pF1KB6917
(Linear) (Six-base) MAPSORT of: KIBB6917.seq Check: 5490 from: 1 to: 615
KIBB6917.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 524 615
Size: 524 91
AccI GT'mk_AC
Cuts at: 0 224 615
Size: 224 391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 615
Size: 458 157
AfeI AGC'GCT
Cuts at: 0 580 615
Size: 580 35
AlwNI CAG_nnn'CTG
Cuts at: 0 507 615
Size: 507 108
AvaI C'yCGr_G
Cuts at: 0 485 615
Size: 485 130
BanI G'GyrC_C
Cuts at: 0 6 159 615
Size: 6 153 456
Fragments arranged by size:
456 153 6
BbeI G_GCGC'C
Cuts at: 0 10 163 615
Size: 10 153 452
Fragments arranged by size:
452 153 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 446 615
Size: 446 169
BglI GCCn_nnn'nGGC
Cuts at: 0 139 615
Size: 139 476
BmgBI CAC'GTC
Cuts at: 0 236 615
Size: 236 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 384 615
Size: 313 71 231
Fragments arranged by size:
313 231 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 615
Size: 210 405
BsaHI Gr'CG_yC
Cuts at: 0 7 148 160 191 615
Size: 7 141 12 31 424
Fragments arranged by size:
424 141 31 12 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 615
Size: 442 173
BseYI C'CCAG_C
Cuts at: 0 317 540 615
Size: 317 223 75
Fragments arranged by size:
317 223 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 502 615
Size: 480 22 113
Fragments arranged by size:
480 113 22
BsiEI CG_ry'CG
Cuts at: 0 95 134 208 615
Size: 95 39 74 407
Fragments arranged by size:
407 95 74 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 119 615
Size: 119 496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 365 524 615
Size: 365 159 91
Fragments arranged by size:
365 159 91
BsrBI CCG'CTC
Cuts at: 0 18 615
Size: 18 597
BssHII G'CGCG_C
Cuts at: 0 62 64 615
Size: 62 2 551
Fragments arranged by size:
551 62 2
BtgI C'CryG_G
Cuts at: 0 30 615
Size: 30 585
DraIII CAC_nnn'GTG
Cuts at: 0 503 615
Size: 503 112
EaeI y'GGCC_r
Cuts at: 0 131 205 338 615
Size: 131 74 133 277
Fragments arranged by size:
277 133 131 74
EagI C'GGCC_G
Cuts at: 0 131 205 615
Size: 131 74 410
Fragments arranged by size:
410 131 74
EarI CTCTTCn'nnn_
Cuts at: 0 49 615
Size: 49 566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 135 615
Size: 135 480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 615
Size: 458 157
EcoNI CCTnn'n_nnAGG
Cuts at: 0 198 615
Size: 198 417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 570 615
Size: 570 45
HaeII r_GCGC'y
Cuts at: 0 10 163 582 615
Size: 10 153 419 33
Fragments arranged by size:
419 153 33 10
HincII GTy'rAC
Cuts at: 0 225 615
Size: 225 390
KasI G'GCGC_C
Cuts at: 0 6 159 615
Size: 6 153 456
Fragments arranged by size:
456 153 6
MspA1I CmG'CkG
Cuts at: 0 317 615
Size: 317 298
NarI GG'CG_CC
Cuts at: 0 7 160 615
Size: 7 153 455
Fragments arranged by size:
455 153 7
NotI GC'GGCC_GC
Cuts at: 0 131 615
Size: 131 484
NruI TCG'CGA
Cuts at: 0 296 615
Size: 296 319
NspI r_CATG'y
Cuts at: 0 494 615
Size: 494 121
PflMI CCAn_nnn'nTGG
Cuts at: 0 336 615
Size: 336 279
PfoI T'CCnGG_A
Cuts at: 0 196 615
Size: 196 419
PvuII CAG'CTG
Cuts at: 0 317 615
Size: 317 298
SalI G'TCGA_C
Cuts at: 0 223 615
Size: 223 392
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 139 615
Size: 139 476
SfoI GGC'GCC
Cuts at: 0 8 161 615
Size: 8 153 454
Fragments arranged by size:
454 153 8
SmaI CCC'GGG
Cuts at: 0 487 615
Size: 487 128
SphI G_CATG'C
Cuts at: 0 494 615
Size: 494 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 485 615
Size: 109 376 130
Fragments arranged by size:
376 130 109
XmaI C'CCGG_G
Cuts at: 0 485 615
Size: 485 130
XmnI GAAnn'nnTTC
Cuts at: 0 43 615
Size: 43 572
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI AvaI BanI BbeI
BbsI BglI BmgBI BmrI BplI BsaHI BseRI BseYI
BsgI BsiEI BsmBI BspMI BsrBI BssHII BtgI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI FalI HaeII
HincII KasI MspA1I NarI NotI NruI NspI PflMI
PfoI PvuII SalI SfiI SfoI SmaI SphI TaqII
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EcoICRI EcoO109I EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI NaeI NcoI NdeI NgoMIV
NheI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI
StuI StyI SwaI TatI TstI Tth111I XbaI XcmI
XhoI ZraI