Restriction map for
pF1KB6919
(Linear) (Six-base) MAPSORT of: KIBB6919.seq Check: 4967 from: 1 to: 651
KIBB6919.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 471 651
Size: 471 180
Acc65I G'GTAC_C
Cuts at: 0 53 651
Size: 53 598
AlwNI CAG_nnn'CTG
Cuts at: 0 22 651
Size: 22 629
BamHI G'GATC_C
Cuts at: 0 41 651
Size: 41 610
BanI G'GyrC_C
Cuts at: 0 53 651
Size: 53 598
BanII G_rGCy'C
Cuts at: 0 341 651
Size: 341 310
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 651
Size: 151 500
BciVI GTATCCnnnnn_n'
Cuts at: 0 562 651
Size: 562 89
BglII A'GATC_T
Cuts at: 0 206 651
Size: 206 445
BlpI GC'TnA_GC
Cuts at: 0 335 651
Size: 335 316
Bme1580I G_kGCm'C
Cuts at: 0 360 651
Size: 360 291
BmrI ACTGGGnnnn_n'
Cuts at: 0 133 651
Size: 133 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 449 651
Size: 172 277 202
Fragments arranged by size:
277 202 172
Bpu10I CC'TnA_GC
Cuts at: 0 293 651
Size: 293 358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 651
Size: 325 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 651
Size: 319 332
BsiHKAI G_wGCw'C
Cuts at: 0 341 651
Size: 341 310
BsmI GAATG_Cn'
Cuts at: 0 412 651
Size: 412 239
BsmBI CGTCTCn'nnnn_
Cuts at: 0 607 651
Size: 607 44
Bsp1286I G_dGCh'C
Cuts at: 0 341 360 651
Size: 341 19 291
Fragments arranged by size:
341 291 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 471 651
Size: 65 406 180
Fragments arranged by size:
406 180 65
BsrFI r'CCGG_y
Cuts at: 0 71 651
Size: 71 580
BsrGI T'GTAC_A
Cuts at: 0 485 651
Size: 485 166
BstAPI GCAn_nnn'nTGC
Cuts at: 0 22 306 651
Size: 22 284 345
Fragments arranged by size:
345 284 22
BstYI r'GATC_y
Cuts at: 0 41 206 651
Size: 41 165 445
Fragments arranged by size:
445 165 41
Bsu36I CC'TnA_GG
Cuts at: 0 49 651
Size: 49 602
BtsI GCAGTG_nn'
Cuts at: 0 287 651
Size: 287 364
DrdI GACnn_nn'nnGTC
Cuts at: 0 259 651
Size: 259 392
EarI CTCTTCn'nnn_
Cuts at: 0 464 517 523 651
Size: 464 53 6 128
Fragments arranged by size:
464 128 53 6
EcoICRI GAG'CTC
Cuts at: 0 339 651
Size: 339 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 449 651
Size: 172 277 202
Fragments arranged by size:
277 202 172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 651
Size: 609 42
KpnI G_GTAC'C
Cuts at: 0 57 651
Size: 57 594
MspA1I CmG'CkG
Cuts at: 0 111 159 651
Size: 111 48 492
Fragments arranged by size:
492 111 48
PfoI T'CCnGG_A
Cuts at: 0 389 651
Size: 389 262
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 61 93 651
Size: 61 32 558
Fragments arranged by size:
558 61 32
PstI C_TGCA'G
Cuts at: 0 62 651
Size: 62 589
PvuII CAG'CTG
Cuts at: 0 159 651
Size: 159 492
SacI G_AGCT'C
Cuts at: 0 341 651
Size: 341 310
SbfI CC_TGCA'GG
Cuts at: 0 62 651
Size: 62 589
ScaI AGT'ACT
Cuts at: 0 505 651
Size: 505 146
SfcI C'TryA_G
Cuts at: 0 58 78 106 417 539 651
Size: 58 20 28 311 122 112
Fragments arranged by size:
311 122 112 58 28 20
SmlI C'TyrA_G
Cuts at: 0 340 651
Size: 340 311
TatI w'GTAC_w
Cuts at: 0 485 503 651
Size: 485 18 148
Fragments arranged by size:
485 148 18
XmnI GAAnn'nnTTC
Cuts at: 0 589 651
Size: 589 62
Enzymes that do cut:
AarI Acc65I AlwNI BamHI BanI BanII BbsI BciVI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BsrGI BstAPI
BstYI Bsu36I BtsI DrdI EarI EcoICRI Eco57MI FalI
KpnI MspA1I PfoI PsrI PstI PvuII SacI SbfI
ScaI SfcI SmlI TatI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI
BclI BfrBI BglI BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI
SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI