(Linear) (Six-base) MAPSORT of: KIBB6920.seq  Check: 6808  from: 1  to: 678



KIBB6920.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

ApoI r'AATT_y

Cuts at:      0     169     678 
   Size:        169     509
 
BlpI GC'TnA_GC

Cuts at:      0     144     678 
   Size:        144     534
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     144     678 
   Size:        144     534
 
Bpu10I CC'TnA_GC

Cuts at:      0     401     678 
   Size:        401     277
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     643     678 
   Size:        275     368      35

  Fragments arranged by size: 

                 368     275      35
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     227     678 
   Size:        227     451
 
BsaBI GATnn'nnATC

Cuts at:      0     466     678 
   Size:        466     212
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     179     209     678 
   Size:        179      30     469

  Fragments arranged by size: 

                 469     179      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     215     512     559     678 
   Size:        215     297      47     119

  Fragments arranged by size: 

                 297     215     119      47
 
BsiHKAI G_wGCw'C

Cuts at:      0     291     678 
   Size:        291     387
 
BsmI GAATG_Cn'

Cuts at:      0     303     678 
   Size:        303     375
 
Bsp1286I G_dGCh'C

Cuts at:      0     291     678 
   Size:        291     387
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     205     678 
   Size:        205     473
 
BsrDI GCAATG_nn'

Cuts at:      0     127     431     678 
   Size:        127     304     247

  Fragments arranged by size: 

                 304     247     127
 
BstBI TT'CG_AA

Cuts at:      0     343     678 
   Size:        343     335
 
EaeI y'GGCC_r

Cuts at:      0      80     249     632     678 
   Size:         80     169     383      46

  Fragments arranged by size: 

                 383     169      80      46
 
EarI CTCTTCn'nnn_

Cuts at:      0      53     233     678 
   Size:         53     180     445

  Fragments arranged by size: 

                 445     180      53
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     468     678 
   Size:        468     210
 
MscI TGG'CCA

Cuts at:      0     634     678 
   Size:        634      44
 
MspA1I CmG'CkG

Cuts at:      0      47     148     678 
   Size:         47     101     530

  Fragments arranged by size: 

                 530     101      47
 
NspI r_CATG'y

Cuts at:      0     143     678 
   Size:        143     535
 
PvuII CAG'CTG

Cuts at:      0     148     678 
   Size:        148     530
 
SfcI C'TryA_G

Cuts at:      0      42     419     678 
   Size:         42     377     259

  Fragments arranged by size: 

                 377     259      42
 
SmlI C'TyrA_G

Cuts at:      0     290     658     678 
   Size:        290     368      20

  Fragments arranged by size: 

                 368     290      20
 
SphI G_CATG'C

Cuts at:      0     143     678 
   Size:        143     535
 
StyI C'CwwG_G

Cuts at:      0     443     579     678 
   Size:        443     136      99

  Fragments arranged by size: 

                 443     136      99
 
TatI w'GTAC_w

Cuts at:      0     319     476     678 
   Size:        319     157     202

  Fragments arranged by size: 

                 319     202     157
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     179     211     678 
   Size:        179      32     467

  Fragments arranged by size: 

                 467     179      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     642     678 
   Size:        642      36
 

 Enzymes that do cut:

     ApoI     BlpI     BmrI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI
    BseRI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BstBI     EaeI
     EarI     FalI     MscI   MspA1I     NspI    PvuII     SfcI     SmlI
     SphI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI     BplI     BpmI
    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI