Restriction map for
pF1KB6921
(Linear) (Six-base) MAPSORT of: KIBB6921.seq Check: 3022 from: 1 to: 615
KIBB6921.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 0 65 569 615
Size: 65 504 46
Fragments arranged by size:
504 65 46
AlwNI CAG_nnn'CTG
Cuts at: 0 74 615
Size: 74 541
ApoI r'AATT_y
Cuts at: 0 385 583 615
Size: 385 198 32
Fragments arranged by size:
385 198 32
BamHI G'GATC_C
Cuts at: 0 543 615
Size: 543 72
BanI G'GyrC_C
Cuts at: 0 562 615
Size: 562 53
BbeI G_GCGC'C
Cuts at: 0 566 615
Size: 566 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 150 615
Size: 150 465
BciVI GTATCCnnnnn_n'
Cuts at: 0 143 615
Size: 143 472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 491 615
Size: 491 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 438 524 615
Size: 233 205 86 91
Fragments arranged by size:
233 205 91 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 201 615
Size: 201 414
BsaBI GATnn'nnATC
Cuts at: 0 187 615
Size: 187 428
BsaHI Gr'CG_yC
Cuts at: 0 56 563 615
Size: 56 507 52
Fragments arranged by size:
507 56 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 615
Size: 422 193
BsrFI r'CCGG_y
Cuts at: 0 36 615
Size: 36 579
BstYI r'GATC_y
Cuts at: 0 543 615
Size: 543 72
BtgI C'CryG_G
Cuts at: 0 49 615
Size: 49 566
DrdI GACnn_nn'nnGTC
Cuts at: 0 61 615
Size: 61 554
EaeI y'GGCC_r
Cuts at: 0 38 615
Size: 38 577
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 467 615
Size: 467 148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 438 524 615
Size: 233 205 86 91
Fragments arranged by size:
233 205 91 86
HaeII r_GCGC'y
Cuts at: 0 566 615
Size: 566 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 615
Size: 212 32 371
Fragments arranged by size:
371 212 32
KasI G'GCGC_C
Cuts at: 0 562 615
Size: 562 53
MslI CAynn'nnrTG
Cuts at: 0 538 615
Size: 538 77
MspA1I CmG'CkG
Cuts at: 0 19 51 74 615
Size: 19 32 23 541
Fragments arranged by size:
541 32 23 19
NaeI GCC'GGC
Cuts at: 0 38 615
Size: 38 577
NarI GG'CG_CC
Cuts at: 0 563 615
Size: 563 52
NgoMIV G'CCGG_C
Cuts at: 0 36 615
Size: 36 579
PvuII CAG'CTG
Cuts at: 0 74 615
Size: 74 541
SacII CC_GC'GG
Cuts at: 0 52 615
Size: 52 563
SfcI C'TryA_G
Cuts at: 0 66 615
Size: 66 549
SfoI GGC'GCC
Cuts at: 0 564 615
Size: 564 51
SspI AAT'ATT
Cuts at: 0 93 615
Size: 93 522
StyI C'CwwG_G
Cuts at: 0 548 615
Size: 548 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 383 449 615
Size: 383 66 166
Fragments arranged by size:
383 166 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 615
Size: 90 32 493
Fragments arranged by size:
493 90 32
XmnI GAAnn'nnTTC
Cuts at: 0 529 615
Size: 529 86
Enzymes that do cut:
AccI AlwNI ApoI BamHI BanI BbeI BbsI BciVI
BplI BpmI BsaI BsaBI BsaHI BsgI BsrFI BstYI
BtgI DrdI EaeI EciI Eco57MI HaeII Hin4I KasI
MslI MspA1I NaeI NarI NgoMIV PvuII SacII SfcI
SfoI SspI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbvCI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EagI EarI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI ZraI