(Linear) (Six-base) MAPSORT of: KIBB6923.seq  Check: 6010  from: 1  to: 615



KIBB6923.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     249     615 
   Size:        249     366
 
ApaLI G'TGCA_C

Cuts at:      0     341     615 
   Size:        341     274
 
ApoI r'AATT_y

Cuts at:      0     599     615 
   Size:        599      16
 
AvaI C'yCGr_G

Cuts at:      0      17     131     615 
   Size:         17     114     484

  Fragments arranged by size: 

                 484     114      17
 
BanII G_rGCy'C

Cuts at:      0     182     615 
   Size:        182     433
 
BclI T'GATC_A

Cuts at:      0     392     615 
   Size:        392     223
 
Bme1580I G_kGCm'C

Cuts at:      0     345     615 
   Size:        345     270
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      87     615 
   Size:         87     528
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     615 
   Size:        196     419
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     615 
   Size:        157     458
 
BsiHKAI G_wGCw'C

Cuts at:      0     182     345     615 
   Size:        182     163     270

  Fragments arranged by size: 

                 270     182     163
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     345     615 
   Size:        182     163     270

  Fragments arranged by size: 

                 270     182     163
 
EaeI y'GGCC_r

Cuts at:      0      59     615 
   Size:         59     556
 
EarI CTCTTCn'nnn_

Cuts at:      0     497     615 
   Size:        497     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     532     615 
   Size:        532      83
 
EcoICRI GAG'CTC

Cuts at:      0     180     615 
   Size:        180     435
 
EcoO109I rG'GnC_Cy

Cuts at:      0     285     615 
   Size:        285     330
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     562     615 
   Size:        562      53
 
HaeII r_GCGC'y

Cuts at:      0       7      50     615 
   Size:          7      43     565

  Fragments arranged by size: 

                 565      43       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     615 
   Size:        413     202
 
PfoI T'CCnGG_A

Cuts at:      0     287     371     615 
   Size:        287      84     244

  Fragments arranged by size: 

                 287     244      84
 
PpuMI rG'GwC_Cy

Cuts at:      0     285     615 
   Size:        285     330
 
PspXI vC'TCGA_Gb

Cuts at:      0     131     615 
   Size:        131     484
 
PstI C_TGCA'G

Cuts at:      0      11     615 
   Size:         11     604
 
SacI G_AGCT'C

Cuts at:      0     182     615 
   Size:        182     433
 
SfcI C'TryA_G

Cuts at:      0       7     615 
   Size:          7     608
 
SmaI CCC'GGG

Cuts at:      0      19     615 
   Size:         19     596
 
SmlI C'TyrA_G

Cuts at:      0     131     175     615 
   Size:        131      44     440

  Fragments arranged by size: 

                 440     131      44
 
SspI AAT'ATT

Cuts at:      0     270     615 
   Size:        270     345
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     360     615 
   Size:        360     255
 
XhoI C'TCGA_G

Cuts at:      0     131     615 
   Size:        131     484
 
XmaI C'CCGG_G

Cuts at:      0      17     615 
   Size:         17     598
 

 Enzymes that do cut:

    AlwNI    ApaLI     ApoI     AvaI    BanII     BclI Bme1580I     BplI
    BpuEI     BsgI  BsiHKAI Bsp1286I     EaeI     EarI     EciI  EcoICRI
 EcoO109I     FalI    HaeII     MmeI     PfoI    PpuMI    PspXI     PstI
     SacI     SfcI     SmaI     SmlI     SspI    TaqII     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI  Eco57MI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XmnI     ZraI