Restriction map for
pF1KB6927
(Linear) (Six-base) MAPSORT of: KIBB6927.seq Check: 7272 from: 1 to: 621
KIBB6927.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 621
Size: 499 122
AflIII A'CryG_T
Cuts at: 0 38 621
Size: 38 583
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 621
Size: 535 32 54
Fragments arranged by size:
535 54 32
AlwNI CAG_nnn'CTG
Cuts at: 0 102 238 621
Size: 102 136 383
Fragments arranged by size:
383 136 102
AvaI C'yCGr_G
Cuts at: 0 44 61 359 614 621
Size: 44 17 298 255 7
Fragments arranged by size:
298 255 44 17 7
AvrII C'CTAG_G
Cuts at: 0 306 621
Size: 306 315
BanI G'GyrC_C
Cuts at: 0 578 621
Size: 578 43
BanII G_rGCy'C
Cuts at: 0 59 213 496 621
Size: 59 154 283 125
Fragments arranged by size:
283 154 125 59
BbeI G_GCGC'C
Cuts at: 0 582 621
Size: 582 39
BglI GCCn_nnn'nGGC
Cuts at: 0 24 235 395 621
Size: 24 211 160 226
Fragments arranged by size:
226 211 160 24
BmgBI CAC'GTC
Cuts at: 0 39 621
Size: 39 582
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 357 621
Size: 357 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 55 97 553 621
Size: 49 6 42 456 68
Fragments arranged by size:
456 68 49 42 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 621
Size: 385 236
BsaHI Gr'CG_yC
Cuts at: 0 579 621
Size: 579 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 621
Size: 535 30 56
Fragments arranged by size:
535 56 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 550 621
Size: 49 501 71
Fragments arranged by size:
501 71 49
BseYI C'CCAG_C
Cuts at: 0 21 621
Size: 21 600
BsiHKAI G_wGCw'C
Cuts at: 0 59 621
Size: 59 562
Bsp1286I G_dGCh'C
Cuts at: 0 59 213 496 621
Size: 59 154 283 125
Fragments arranged by size:
283 154 125 59
BsrBI CCG'CTC
Cuts at: 0 33 66 621
Size: 33 33 555
Fragments arranged by size:
555 33 33
BsrFI r'CCGG_y
Cuts at: 0 190 575 621
Size: 190 385 46
Fragments arranged by size:
385 190 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 102 621
Size: 102 519
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 621
Size: 279 342
BstYI r'GATC_y
Cuts at: 0 203 621
Size: 203 418
BtgI C'CryG_G
Cuts at: 0 390 621
Size: 390 231
EcoICRI GAG'CTC
Cuts at: 0 57 621
Size: 57 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 55 97 499 553 621
Size: 49 6 42 402 54 68
Fragments arranged by size:
402 68 54 49 42 6
EcoO109I rG'GnC_Cy
Cuts at: 0 108 303 621
Size: 108 195 318
Fragments arranged by size:
318 195 108
HaeII r_GCGC'y
Cuts at: 0 200 582 621
Size: 200 382 39
Fragments arranged by size:
382 200 39
KasI G'GCGC_C
Cuts at: 0 578 621
Size: 578 43
MspA1I CmG'CkG
Cuts at: 0 21 80 621
Size: 21 59 541
Fragments arranged by size:
541 59 21
NaeI GCC'GGC
Cuts at: 0 577 621
Size: 577 44
NarI GG'CG_CC
Cuts at: 0 579 621
Size: 579 42
NcoI C'CATG_G
Cuts at: 0 390 621
Size: 390 231
NgoMIV G'CCGG_C
Cuts at: 0 575 621
Size: 575 46
PasI CC'CwG_GG
Cuts at: 0 272 370 489 621
Size: 272 98 119 132
Fragments arranged by size:
272 132 119 98
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 621
Size: 535 32 54
Fragments arranged by size:
535 54 32
PspXI vC'TCGA_Gb
Cuts at: 0 61 621
Size: 61 560
PstI C_TGCA'G
Cuts at: 0 107 128 621
Size: 107 21 493
Fragments arranged by size:
493 107 21
SacI G_AGCT'C
Cuts at: 0 59 621
Size: 59 562
SfcI C'TryA_G
Cuts at: 0 103 124 621
Size: 103 21 497
Fragments arranged by size:
497 103 21
SfoI GGC'GCC
Cuts at: 0 580 621
Size: 580 41
SgrAI Cr'CCGG_yG
Cuts at: 0 575 621
Size: 575 46
SmaI CCC'GGG
Cuts at: 0 46 361 621
Size: 46 315 260
Fragments arranged by size:
315 260 46
SmlI C'TyrA_G
Cuts at: 0 61 400 614 621
Size: 61 339 214 7
Fragments arranged by size:
339 214 61 7
StuI AGG'CCT
Cuts at: 0 398 621
Size: 398 223
StyI C'CwwG_G
Cuts at: 0 224 306 390 605 621
Size: 224 82 84 215 16
Fragments arranged by size:
224 215 84 82 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 621
Size: 313 308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 621
Size: 231 390
XhoI C'TCGA_G
Cuts at: 0 61 614 621
Size: 61 553 7
Fragments arranged by size:
553 61 7
XmaI C'CCGG_G
Cuts at: 0 44 359 621
Size: 44 315 262
Fragments arranged by size:
315 262 44
Enzymes that do cut:
AcuI AflIII AloI AlwNI AvaI AvrII BanI BanII
BbeI BglI BmgBI BplI BpmI BpuEI BsaHI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BsrBI BsrFI BstAPI BstXI
BstYI BtgI EcoICRI Eco57MI EcoO109I HaeII KasI MspA1I
NaeI NarI NcoI NgoMIV PasI PpiI PspXI PstI
SacI SfcI SfoI SgrAI SmaI SmlI StuI StyI
TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI Bpu10I BsaI
BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XmnI ZraI