(Linear) (Six-base) MAPSORT of: KIBB6932.seq Check: 5295 from: 1 to: 684 KIBB6932.orf With 182 enzymes: SgfI * April 24, 2009 13:07 .. AclI AA'CG_TT Cuts at: 0 669 684 Size: 669 15 AlwNI CAG_nnn'CTG Cuts at: 0 658 684 Size: 658 26 ApoI r'AATT_y Cuts at: 0 451 684 Size: 451 233 AvaI C'yCGr_G Cuts at: 0 283 684 Size: 283 401 BanI G'GyrC_C Cuts at: 0 384 684 Size: 384 300 BbsI GAAGACnn'nnnn_ Cuts at: 0 617 684 Size: 617 67 BclI T'GATC_A Cuts at: 0 626 684 Size: 626 58 BglII A'GATC_T Cuts at: 0 619 684 Size: 619 65 BmrI ACTGGGnnnn_n' Cuts at: 0 489 684 Size: 489 195 BsaHI Gr'CG_yC Cuts at: 0 4 684 Size: 4 680 BsaWI w'CCGG_w Cuts at: 0 71 684 Size: 71 613 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 618 684 Size: 618 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 578 684 Size: 85 493 106 Fragments arranged by size: 493 106 85 BspEI T'CCGG_A Cuts at: 0 71 684 Size: 71 613 BstBI TT'CG_AA Cuts at: 0 171 684 Size: 171 513 BstEII G'GTnAC_C Cuts at: 0 148 684 Size: 148 536 BstXI CCAn_nnnn'nTGG Cuts at: 0 233 684 Size: 233 451 BstYI r'GATC_y Cuts at: 0 619 684 Size: 619 65 BtgI C'CryG_G Cuts at: 0 388 684 Size: 388 296 BtsI GCAGTG_nn' Cuts at: 0 597 684 Size: 597 87 DraIII CAC_nnn'GTG Cuts at: 0 156 684 Size: 156 528 EaeI y'GGCC_r Cuts at: 0 47 684 Size: 47 637 EcoO109I rG'GnC_Cy Cuts at: 0 189 491 535 684 Size: 189 302 44 149 Fragments arranged by size: 302 189 149 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 604 611 636 643 684 Size: 604 7 25 7 41 Fragments arranged by size: 604 41 25 7 7 MscI TGG'CCA Cuts at: 0 49 684 Size: 49 635 MslI CAynn'nnrTG Cuts at: 0 208 214 554 580 684 Size: 208 6 340 26 104 Fragments arranged by size: 340 208 104 26 6 NcoI C'CATG_G Cuts at: 0 388 684 Size: 388 296 NspI r_CATG'y Cuts at: 0 207 684 Size: 207 477 PasI CC'CwG_GG Cuts at: 0 301 684 Size: 301 383 PpuMI rG'GwC_Cy Cuts at: 0 189 491 535 684 Size: 189 302 44 149 Fragments arranged by size: 302 189 149 44 SfcI C'TryA_G Cuts at: 0 251 684 Size: 251 433 SmlI C'TyrA_G Cuts at: 0 283 684 Size: 283 401 StyI C'CwwG_G Cuts at: 0 388 413 684 Size: 388 25 271 Fragments arranged by size: 388 271 25 TatI w'GTAC_w Cuts at: 0 269 684 Size: 269 415 Tth111I GACn'n_nGTC Cuts at: 0 544 684 Size: 544 140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 479 684 Size: 479 205 XhoI C'TCGA_G Cuts at: 0 283 684 Size: 283 401 XmnI GAAnn'nnTTC Cuts at: 0 167 684 Size: 167 517 Enzymes that do cut: AclI AlwNI ApoI AvaI BanI BbsI BclI BglII BmrI BsaHI BsaWI BseRI BsgI BspEI BstBI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EcoO109I Hin4I MscI MslI NcoI NspI PasI PpuMI SfcI SmlI StyI TatI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI XbaI XmaI ZraI