(Linear) (Six-base) MAPSORT of: KIBB6932.seq Check: 5295 from: 1 to: 684
KIBB6932.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AclI AA'CG_TT
Cuts at: 0 669 684
Size: 669 15
AlwNI CAG_nnn'CTG
Cuts at: 0 658 684
Size: 658 26
ApoI r'AATT_y
Cuts at: 0 451 684
Size: 451 233
AvaI C'yCGr_G
Cuts at: 0 283 684
Size: 283 401
BanI G'GyrC_C
Cuts at: 0 384 684
Size: 384 300
BbsI GAAGACnn'nnnn_
Cuts at: 0 617 684
Size: 617 67
BclI T'GATC_A
Cuts at: 0 626 684
Size: 626 58
BglII A'GATC_T
Cuts at: 0 619 684
Size: 619 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 489 684
Size: 489 195
BsaHI Gr'CG_yC
Cuts at: 0 4 684
Size: 4 680
BsaWI w'CCGG_w
Cuts at: 0 71 684
Size: 71 613
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 618 684
Size: 618 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 578 684
Size: 85 493 106
Fragments arranged by size:
493 106 85
BspEI T'CCGG_A
Cuts at: 0 71 684
Size: 71 613
BstBI TT'CG_AA
Cuts at: 0 171 684
Size: 171 513
BstEII G'GTnAC_C
Cuts at: 0 148 684
Size: 148 536
BstXI CCAn_nnnn'nTGG
Cuts at: 0 233 684
Size: 233 451
BstYI r'GATC_y
Cuts at: 0 619 684
Size: 619 65
BtgI C'CryG_G
Cuts at: 0 388 684
Size: 388 296
BtsI GCAGTG_nn'
Cuts at: 0 597 684
Size: 597 87
DraIII CAC_nnn'GTG
Cuts at: 0 156 684
Size: 156 528
EaeI y'GGCC_r
Cuts at: 0 47 684
Size: 47 637
EcoO109I rG'GnC_Cy
Cuts at: 0 189 491 535 684
Size: 189 302 44 149
Fragments arranged by size:
302 189 149 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 611 636 643 684
Size: 604 7 25 7 41
Fragments arranged by size:
604 41 25 7 7
MscI TGG'CCA
Cuts at: 0 49 684
Size: 49 635
MslI CAynn'nnrTG
Cuts at: 0 208 214 554 580 684
Size: 208 6 340 26 104
Fragments arranged by size:
340 208 104 26 6
NcoI C'CATG_G
Cuts at: 0 388 684
Size: 388 296
NspI r_CATG'y
Cuts at: 0 207 684
Size: 207 477
PasI CC'CwG_GG
Cuts at: 0 301 684
Size: 301 383
PpuMI rG'GwC_Cy
Cuts at: 0 189 491 535 684
Size: 189 302 44 149
Fragments arranged by size:
302 189 149 44
SfcI C'TryA_G
Cuts at: 0 251 684
Size: 251 433
SmlI C'TyrA_G
Cuts at: 0 283 684
Size: 283 401
StyI C'CwwG_G
Cuts at: 0 388 413 684
Size: 388 25 271
Fragments arranged by size:
388 271 25
TatI w'GTAC_w
Cuts at: 0 269 684
Size: 269 415
Tth111I GACn'n_nGTC
Cuts at: 0 544 684
Size: 544 140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 479 684
Size: 479 205
XhoI C'TCGA_G
Cuts at: 0 283 684
Size: 283 401
XmnI GAAnn'nnTTC
Cuts at: 0 167 684
Size: 167 517
Enzymes that do cut:
AclI AlwNI ApoI AvaI BanI BbsI BclI BglII
BmrI BsaHI BsaWI BseRI BsgI BspEI BstBI BstEII
BstXI BstYI BtgI BtsI DraIII EaeI EcoO109I Hin4I
MscI MslI NcoI NspI PasI PpuMI SfcI SmlI
StyI TatI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI
BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI XbaI XmaI ZraI