(Linear) (Six-base) MAPSORT of: KIBB6933.seq  Check: 905  from: 1  to: 621



KIBB6933.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     116     621 
   Size:        116     505
 
AlwNI CAG_nnn'CTG

Cuts at:      0      94     621 
   Size:         94     527
 
ApoI r'AATT_y

Cuts at:      0     194     621 
   Size:        194     427
 
BamHI G'GATC_C

Cuts at:      0     528     621 
   Size:        528      93
 
BanII G_rGCy'C

Cuts at:      0      37     621 
   Size:         37     584
 
BmtI G_CTAG'C

Cuts at:      0     211     621 
   Size:        211     410
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     477     621 
   Size:         71     406     144

  Fragments arranged by size: 

                 406     144      71
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      68     621 
   Size:         68     553
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     221     621 
   Size:        221     400
 
BseYI C'CCAG_C

Cuts at:      0      37      78     128     247     621 
   Size:         37      41      50     119     374

  Fragments arranged by size: 

                 374     119      50      41      37
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     621 
   Size:         37     584
 
BsrBI CCG'CTC

Cuts at:      0      85     621 
   Size:         85     536
 
BstYI r'GATC_y

Cuts at:      0     528     621 
   Size:        528      93
 
BtsI GCAGTG_nn'

Cuts at:      0      54     164     249     402     621 
   Size:         54     110      85     153     219

  Fragments arranged by size: 

                 219     153     110      85      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     116     477     621 
   Size:         71      45     361     144

  Fragments arranged by size: 

                 361     144      71      45
 
EcoRI G'AATT_C

Cuts at:      0     194     621 
   Size:        194     427
 
EcoRV GAT'ATC

Cuts at:      0     339     621 
   Size:        339     282
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     134     621 
   Size:        134     487
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     519     621 
   Size:        519     102
 
NheI G'CTAG_C

Cuts at:      0     207     621 
   Size:        207     414
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     599     621 
   Size:        599      22
 
SpeI A'CTAG_T

Cuts at:      0     181     621 
   Size:        181     440
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     142     621 
   Size:        142     479
 
XmnI GAAnn'nnTTC

Cuts at:      0     117     621 
   Size:        117     504
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BamHI    BanII     BmtI     BpmI     BsaI
    BseRI    BseYI Bsp1286I    BsrBI    BstYI     BtsI  Eco57MI    EcoRI
    EcoRV     FalI     MmeI     NheI    PflMI     SpeI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI