(Linear) (Six-base) MAPSORT of: KIBB6934.seq  Check: 8420  from: 1  to: 657



KIBB6934.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AccI GT'mk_AC

Cuts at:      0     438     657 
   Size:        438     219
 
AclI AA'CG_TT

Cuts at:      0     486     657 
   Size:        486     171
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     577     657 
   Size:        456     121      80

  Fragments arranged by size: 

                 456     121      80
 
AflIII A'CryG_T

Cuts at:      0     174     657 
   Size:        174     483
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     104     657 
   Size:         75      29     553

  Fragments arranged by size: 

                 553      75      29
 
ApoI r'AATT_y

Cuts at:      0     550     657 
   Size:        550     107
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     426     657 
   Size:        426     231
 
BsaAI yAC'GTr

Cuts at:      0     177     657 
   Size:        177     480
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     657 
   Size:         19     638
 
BsiEI CG_ry'CG

Cuts at:      0      89     657 
   Size:         89     568
 
BsiHKAI G_wGCw'C

Cuts at:      0     637     657 
   Size:        637      20
 
Bsp1286I G_dGCh'C

Cuts at:      0     637     657 
   Size:        637      20
 
BsrDI GCAATG_nn'

Cuts at:      0     138     144     156     657 
   Size:        138       6      12     501

  Fragments arranged by size: 

                 501     138      12       6
 
BsrFI r'CCGG_y

Cuts at:      0      84      88     657 
   Size:         84       4     569

  Fragments arranged by size: 

                 569      84       4
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      75     657 
   Size:         75     582
 
EaeI y'GGCC_r

Cuts at:      0      86     657 
   Size:         86     571
 
EagI C'GGCC_G

Cuts at:      0      86     657 
   Size:         86     571
 
EarI CTCTTCn'nnn_

Cuts at:      0     583     657 
   Size:        583      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     577     657 
   Size:        456     121      80

  Fragments arranged by size: 

                 456     121      80
 
FseI GG_CCGG'CC

Cuts at:      0      92     657 
   Size:         92     565
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     334     366     406     438     657 
   Size:        334      32      40      32     219

  Fragments arranged by size: 

                 334     219      40      32      32
 
NaeI GCC'GGC

Cuts at:      0      86      90     657 
   Size:         86       4     567

  Fragments arranged by size: 

                 567      86       4
 
NgoMIV G'CCGG_C

Cuts at:      0      84      88     657 
   Size:         84       4     569

  Fragments arranged by size: 

                 569      84       4
 
StyI C'CwwG_G

Cuts at:      0     458     657 
   Size:        458     199
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      96     471     657 
   Size:         96     375     186

  Fragments arranged by size: 

                 375     186      96
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     166     198     657 
   Size:        166      32     459

  Fragments arranged by size: 

                 459     166      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     449     562     657 
   Size:        449     113      95

  Fragments arranged by size: 

                 449     113      95
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII    AlwNI     ApoI     BmrI    BsaAI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrDI    BsrFI   BstAPI     EaeI
     EagI     EarI  Eco57MI     FseI    Hin4I     NaeI   NgoMIV     StyI
    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI