Restriction map for
pF1KB6935
(Linear) (Six-base) MAPSORT of: KIBB6935.seq Check: 7652 from: 1 to: 621
KIBB6935.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AatII G_ACGT'C
Cuts at: 0 115 621
Size: 115 506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 621
Size: 328 293
AgeI A'CCGG_T
Cuts at: 0 521 621
Size: 521 100
AlwNI CAG_nnn'CTG
Cuts at: 0 81 621
Size: 81 540
ApaLI G'TGCA_C
Cuts at: 0 183 621
Size: 183 438
ApoI r'AATT_y
Cuts at: 0 319 621
Size: 319 302
AvaI C'yCGr_G
Cuts at: 0 217 594 621
Size: 217 377 27
Fragments arranged by size:
377 217 27
BanII G_rGCy'C
Cuts at: 0 173 553 601 621
Size: 173 380 48 20
Fragments arranged by size:
380 173 48 20
Bme1580I G_kGCm'C
Cuts at: 0 187 621
Size: 187 434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 621
Size: 76 545
Bpu10I CC'TnA_GC
Cuts at: 0 552 621
Size: 552 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 621
Size: 559 62
BsaHI Gr'CG_yC
Cuts at: 0 112 621
Size: 112 509
BsaWI w'CCGG_w
Cuts at: 0 165 475 521 621
Size: 165 310 46 100
Fragments arranged by size:
310 165 100 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 540 570 621
Size: 540 30 51
Fragments arranged by size:
540 51 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 407 621
Size: 407 214
BseYI C'CCAG_C
Cuts at: 0 138 621
Size: 138 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 621
Size: 67 554
BsiHKAI G_wGCw'C
Cuts at: 0 187 553 621
Size: 187 366 68
Fragments arranged by size:
366 187 68
BsmI GAATG_Cn'
Cuts at: 0 204 621
Size: 204 417
Bsp1286I G_dGCh'C
Cuts at: 0 173 187 553 601 621
Size: 173 14 366 48 20
Fragments arranged by size:
366 173 48 20 14
BspEI T'CCGG_A
Cuts at: 0 165 475 621
Size: 165 310 146
Fragments arranged by size:
310 165 146
BsrBI CCG'CTC
Cuts at: 0 583 621
Size: 583 38
BsrDI GCAATG_nn'
Cuts at: 0 404 621
Size: 404 217
BsrFI r'CCGG_y
Cuts at: 0 243 521 621
Size: 243 278 100
Fragments arranged by size:
278 243 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 124 621
Size: 81 43 497
Fragments arranged by size:
497 81 43
BtgI C'CryG_G
Cuts at: 0 128 461 621
Size: 128 333 160
Fragments arranged by size:
333 160 128
BtsI GCAGTG_nn'
Cuts at: 0 187 392 621
Size: 187 205 229
Fragments arranged by size:
229 205 187
DraIII CAC_nnn'GTG
Cuts at: 0 441 621
Size: 441 180
EaeI y'GGCC_r
Cuts at: 0 485 612 621
Size: 485 127 9
Fragments arranged by size:
485 127 9
EarI CTCTTCn'nnn_
Cuts at: 0 224 254 621
Size: 224 30 367
Fragments arranged by size:
367 224 30
EcoICRI GAG'CTC
Cuts at: 0 551 621
Size: 551 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 328 621
Size: 76 252 293
Fragments arranged by size:
293 252 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 621
Size: 103 518
EcoO109I rG'GnC_Cy
Cuts at: 0 135 566 621
Size: 135 431 55
Fragments arranged by size:
431 135 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 555 621
Size: 555 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 540 572 621
Size: 393 32 115 32 49
Fragments arranged by size:
393 115 49 32 32
MspA1I CmG'CkG
Cuts at: 0 121 148 455 621
Size: 121 27 307 166
Fragments arranged by size:
307 166 121 27
NaeI GCC'GGC
Cuts at: 0 245 621
Size: 245 376
NgoMIV G'CCGG_C
Cuts at: 0 243 621
Size: 243 378
PflMI CCAn_nnn'nTGG
Cuts at: 0 441 525 621
Size: 441 84 96
Fragments arranged by size:
441 96 84
PpuMI rG'GwC_Cy
Cuts at: 0 135 621
Size: 135 486
PstI C_TGCA'G
Cuts at: 0 120 451 621
Size: 120 331 170
Fragments arranged by size:
331 170 120
PvuII CAG'CTG
Cuts at: 0 121 621
Size: 121 500
SacI G_AGCT'C
Cuts at: 0 553 621
Size: 553 68
SapI GCTCTTCn'nnn_
Cuts at: 0 254 621
Size: 254 367
SfcI C'TryA_G
Cuts at: 0 116 447 621
Size: 116 331 174
Fragments arranged by size:
331 174 116
SmlI C'TyrA_G
Cuts at: 0 574 621
Size: 574 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 473 505 621
Size: 473 32 116
Fragments arranged by size:
473 116 32
XmnI GAAnn'nnTTC
Cuts at: 0 500 621
Size: 500 121
ZraI GAC'GTC
Cuts at: 0 113 621
Size: 113 508
Enzymes that do cut:
AatII AcuI AgeI AlwNI ApaLI ApoI AvaI BanII
Bme1580I BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI
BsrFI BstAPI BtgI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI Hin4I MspA1I NaeI NgoMIV
PflMI PpuMI PstI PvuII SacI SapI SfcI SmlI
TstI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI
BspMI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI