(Linear) (Six-base) MAPSORT of: KIBB6938.seq  Check: 4493  from: 1  to: 687



KIBB6938.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     433     687 
   Size:        333     100     254

  Fragments arranged by size: 

                 333     254     100
 
AfeI AGC'GCT

Cuts at:      0     235     687 
   Size:        235     452
 
AlwNI CAG_nnn'CTG

Cuts at:      0     302     687 
   Size:        302     385
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      10     687 
   Size:         10     677
 
Bme1580I G_kGCm'C

Cuts at:      0     277     687 
   Size:        277     410
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     540     687 
   Size:        540     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336     687 
   Size:        336     351
 
BsaWI w'CCGG_w

Cuts at:      0     425     687 
   Size:        425     262
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      69     687 
   Size:         69     618
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     687 
   Size:        372     315
 
BsmI GAATG_Cn'

Cuts at:      0     405     687 
   Size:        405     282
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      63     687 
   Size:         63     624
 
Bsp1286I G_dGCh'C

Cuts at:      0     277     687 
   Size:        277     410
 
EarI CTCTTCn'nnn_

Cuts at:      0     365     687 
   Size:        365     322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     336     433     687 
   Size:        333       3      97     254

  Fragments arranged by size: 

                 333     254      97       3
 
HaeII r_GCGC'y

Cuts at:      0     237     687 
   Size:        237     450
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     687 
   Size:        456     231
 
MslI CAynn'nnrTG

Cuts at:      0     394     687 
   Size:        394     293
 
ScaI AGT'ACT

Cuts at:      0     385     687 
   Size:        385     302
 
SspI AAT'ATT

Cuts at:      0      33     687 
   Size:         33     654
 
StyI C'CwwG_G

Cuts at:      0     668     687 
   Size:        668      19
 
TatI w'GTAC_w

Cuts at:      0     383     440     687 
   Size:        383      57     247

  Fragments arranged by size: 

                 383     247      57
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI    BciVI Bme1580I     BplI     BpmI    BsaWI
    BseRI     BsgI     BsmI    BsmBI Bsp1286I     EarI  Eco57MI    HaeII
     MmeI     MslI     ScaI     SspI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI