(Linear) (Six-base) MAPSORT of: KIBB6941.seq  Check: 4091  from: 1  to: 627



KIBB6941.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     548     605     627 
   Size:         97     451      57      22

  Fragments arranged by size: 

                 451      97      57      22
 
AflII C'TTAA_G

Cuts at:      0     340     627 
   Size:        340     287
 
AflIII A'CryG_T

Cuts at:      0     438     577     627 
   Size:        438     139      50

  Fragments arranged by size: 

                 438     139      50
 
ApoI r'AATT_y

Cuts at:      0     517     627 
   Size:        517     110
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     566     627 
   Size:        566      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       2     627 
   Size:          2     625
 
BsaAI yAC'GTr

Cuts at:      0     578     627 
   Size:        578      49
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     251     627 
   Size:        251     376
 
BsrGI T'GTAC_A

Cuts at:      0     441     627 
   Size:        441     186
 
BstYI r'GATC_y

Cuts at:      0     103     627 
   Size:        103     524
 
EarI CTCTTCn'nnn_

Cuts at:      0     111     627 
   Size:        111     516
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     548     605     627 
   Size:         97     451      57      22

  Fragments arranged by size: 

                 451      97      57      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     403     627 
   Size:        403     224
 
HaeII r_GCGC'y

Cuts at:      0     328     627 
   Size:        328     299
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     627 
   Size:         93     534
 
MslI CAynn'nnrTG

Cuts at:      0     161     627 
   Size:        161     466
 
NspI r_CATG'y

Cuts at:      0     442     627 
   Size:        442     185
 
PasI CC'CwG_GG

Cuts at:      0     193     627 
   Size:        193     434
 
PciI A'CATG_T

Cuts at:      0     438     627 
   Size:        438     189
 
PmlI CAC'GTG

Cuts at:      0     578     627 
   Size:        578      49
 
SmlI C'TyrA_G

Cuts at:      0     340     627 
   Size:        340     287
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     259     627 
   Size:        259     368
 
TatI w'GTAC_w

Cuts at:      0     441     627 
   Size:        441     186
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     ApoI     BbsI     BmrI    BsaAI    BseRI
    BsrGI    BstYI     EarI  Eco57MI    EcoNI    HaeII     MmeI     MslI
     NspI     PasI     PciI     PmlI     SmlI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI