Restriction map for
pF1KB6942
(Linear) (Six-base) MAPSORT of: KIBB6942.seq Check: 1066 from: 1 to: 687
KIBB6942.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 147 687
Size: 147 540
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 687
Size: 685 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 512 687
Size: 512 175
AlwNI CAG_nnn'CTG
Cuts at: 0 499 687
Size: 499 188
ApaI G_GGCC'C
Cuts at: 0 175 687
Size: 175 512
BanI G'GyrC_C
Cuts at: 0 40 135 687
Size: 40 95 552
Fragments arranged by size:
552 95 40
BanII G_rGCy'C
Cuts at: 0 175 687
Size: 175 512
BglI GCCn_nnn'nGGC
Cuts at: 0 49 82 687
Size: 49 33 605
Fragments arranged by size:
605 49 33
Bme1580I G_kGCm'C
Cuts at: 0 45 138 175 687
Size: 45 93 37 512
Fragments arranged by size:
512 93 45 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 687
Size: 427 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 550 687
Size: 550 137
BsaAI yAC'GTr
Cuts at: 0 516 687
Size: 516 171
BsaHI Gr'CG_yC
Cuts at: 0 673 687
Size: 673 14
BsaWI w'CCGG_w
Cuts at: 0 461 687
Size: 461 226
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 337 424 541 568 687
Size: 73 264 87 117 27 119
Fragments arranged by size:
264 119 117 87 73 27
BseYI C'CCAG_C
Cuts at: 0 86 587 687
Size: 86 501 100
Fragments arranged by size:
501 100 86
BsiEI CG_ry'CG
Cuts at: 0 74 687
Size: 74 613
BsiHKAI G_wGCw'C
Cuts at: 0 656 687
Size: 656 31
BsmI GAATG_Cn'
Cuts at: 0 478 687
Size: 478 209
Bsp1286I G_dGCh'C
Cuts at: 0 45 138 175 656 687
Size: 45 93 37 481 31
Fragments arranged by size:
481 93 45 37 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 687
Size: 147 540
BsrBI CCG'CTC
Cuts at: 0 38 687
Size: 38 649
BsrDI GCAATG_nn'
Cuts at: 0 266 687
Size: 266 421
BsrFI r'CCGG_y
Cuts at: 0 142 687
Size: 142 545
BssSI C'ACGA_G
Cuts at: 0 177 687
Size: 177 510
BstAPI GCAn_nnn'nTGC
Cuts at: 0 268 623 687
Size: 268 355 64
Fragments arranged by size:
355 268 64
BtsI GCAGTG_nn'
Cuts at: 0 628 687
Size: 628 59
DraIII CAC_nnn'GTG
Cuts at: 0 572 687
Size: 572 115
DrdI GACnn_nn'nnGTC
Cuts at: 0 678 687
Size: 678 9
EaeI y'GGCC_r
Cuts at: 0 71 687
Size: 71 616
EagI C'GGCC_G
Cuts at: 0 71 687
Size: 71 616
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 687
Size: 70 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 685 687
Size: 427 258 2
Fragments arranged by size:
427 258 2
EcoO109I rG'GnC_Cy
Cuts at: 0 63 172 402 687
Size: 63 109 230 285
Fragments arranged by size:
285 230 109 63
EcoRV GAT'ATC
Cuts at: 0 504 687
Size: 504 183
HaeII r_GCGC'y
Cuts at: 0 426 687
Size: 426 261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 687
Size: 343 32 312
Fragments arranged by size:
343 312 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 687
Size: 11 676
NaeI GCC'GGC
Cuts at: 0 144 687
Size: 144 543
NgoMIV G'CCGG_C
Cuts at: 0 142 687
Size: 142 545
NotI GC'GGCC_GC
Cuts at: 0 71 687
Size: 71 616
PpuMI rG'GwC_Cy
Cuts at: 0 63 402 687
Size: 63 339 285
Fragments arranged by size:
339 285 63
PshAI GACnn'nnGTC
Cuts at: 0 211 687
Size: 211 476
PspOMI G'GGCC_C
Cuts at: 0 171 687
Size: 171 516
StyI C'CwwG_G
Cuts at: 0 548 687
Size: 548 139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 402 569 687
Size: 402 167 118
Fragments arranged by size:
402 167 118
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI BanI BanII BglI
Bme1580I BpmI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BtsI DraIII DrdI EaeI EagI EciI
Eco57MI EcoO109I EcoRV HaeII Hin4I MmeI NaeI NgoMIV
NotI PpuMI PshAI PspOMI StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaBI BsaXI BsgI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI EarI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
MspA1I NarI NcoI NdeI NheI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI