Restriction map for
pF1KB6949
(Linear) (Six-base) MAPSORT of: KIBB6949.seq Check: 1106 from: 1 to: 633
KIBB6949.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
Acc65I G'GTAC_C
Cuts at: 0 581 633
Size: 581 52
AlwNI CAG_nnn'CTG
Cuts at: 0 24 514 633
Size: 24 490 119
Fragments arranged by size:
490 119 24
ApaLI G'TGCA_C
Cuts at: 0 309 536 587 633
Size: 309 227 51 46
Fragments arranged by size:
309 227 51 46
AvaI C'yCGr_G
Cuts at: 0 239 633
Size: 239 394
BanI G'GyrC_C
Cuts at: 0 581 633
Size: 581 52
BanII G_rGCy'C
Cuts at: 0 100 246 633
Size: 100 146 387
Fragments arranged by size:
387 146 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 254 633
Size: 220 34 379
Fragments arranged by size:
379 220 34
BglI GCCn_nnn'nGGC
Cuts at: 0 232 633
Size: 232 401
Bme1580I G_kGCm'C
Cuts at: 0 313 540 591 633
Size: 313 227 51 42
Fragments arranged by size:
313 227 51 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 261 633
Size: 261 372
BseYI C'CCAG_C
Cuts at: 0 24 45 50 267 503 577 633
Size: 24 21 5 217 236 74 56
Fragments arranged by size:
236 217 74 56 24 21 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 633
Size: 54 579
BsiHKAI G_wGCw'C
Cuts at: 0 100 175 212 313 540 591 633
Size: 100 75 37 101 227 51 42
Fragments arranged by size:
227 101 100 75 51 42 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 320 633
Size: 320 313
Bsp1286I G_dGCh'C
Cuts at: 0 100 175 212 246 313 540 591 633
Size: 100 75 37 34 67 227 51 42
Fragments arranged by size:
227 100 75 67 51 42 37 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 376 633
Size: 376 257
BsrBI CCG'CTC
Cuts at: 0 596 633
Size: 596 37
BsrGI T'GTAC_A
Cuts at: 0 137 633
Size: 137 496
BstAPI GCAn_nnn'nTGC
Cuts at: 0 308 391 514 633
Size: 308 83 123 119
Fragments arranged by size:
308 123 119 83
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 633
Size: 288 345
BtgI C'CryG_G
Cuts at: 0 35 221 356 633
Size: 35 186 135 277
Fragments arranged by size:
277 186 135 35
BtsI GCAGTG_nn'
Cuts at: 0 91 538 633
Size: 91 447 95
Fragments arranged by size:
447 95 91
EaeI y'GGCC_r
Cuts at: 0 2 278 290 420 633
Size: 2 276 12 130 213
Fragments arranged by size:
276 213 130 12 2
EarI CTCTTCn'nnn_
Cuts at: 0 621 633
Size: 621 12
EcoICRI GAG'CTC
Cuts at: 0 98 633
Size: 98 535
KpnI G_GTAC'C
Cuts at: 0 585 633
Size: 585 48
MscI TGG'CCA
Cuts at: 0 4 280 292 422 633
Size: 4 276 12 130 211
Fragments arranged by size:
276 211 130 12 4
MslI CAynn'nnrTG
Cuts at: 0 178 286 633
Size: 178 108 347
Fragments arranged by size:
347 178 108
MspA1I CmG'CkG
Cuts at: 0 223 633
Size: 223 410
NcoI C'CATG_G
Cuts at: 0 35 356 633
Size: 35 321 277
Fragments arranged by size:
321 277 35
PsiI TTA'TAA
Cuts at: 0 446 633
Size: 446 187
PspXI vC'TCGA_Gb
Cuts at: 0 239 633
Size: 239 394
PstI C_TGCA'G
Cuts at: 0 20 633
Size: 20 613
SacI G_AGCT'C
Cuts at: 0 100 633
Size: 100 533
SacII CC_GC'GG
Cuts at: 0 224 633
Size: 224 409
SfcI C'TryA_G
Cuts at: 0 16 633
Size: 16 617
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 232 633
Size: 232 401
SmlI C'TyrA_G
Cuts at: 0 239 633
Size: 239 394
StyI C'CwwG_G
Cuts at: 0 35 356 398 620 633
Size: 35 321 42 222 13
Fragments arranged by size:
321 222 42 35 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 42 349 633
Size: 42 307 284
Fragments arranged by size:
307 284 42
TatI w'GTAC_w
Cuts at: 0 137 633
Size: 137 496
Tth111I GACn'n_nGTC
Cuts at: 0 160 633
Size: 160 473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 633
Size: 42 591
XhoI C'TCGA_G
Cuts at: 0 239 633
Size: 239 394
Enzymes that do cut:
Acc65I AlwNI ApaLI AvaI BanI BanII BcgI BglI
Bme1580I BsaI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrGI BstAPI BstXI BtgI BtsI EaeI EarI
EcoICRI KpnI MscI MslI MspA1I NcoI PsiI PspXI
PstI SacI SacII SfcI SfiI SmlI StyI TaqII
TatI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XmaI XmnI ZraI