Restriction map for
pF1KB6950
(Linear) (Six-base) MAPSORT of: KIBB6950.seq Check: 6117 from: 1 to: 702
KIBB6950.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 702
Size: 692 10
ApaI G_GGCC'C
Cuts at: 0 241 702
Size: 241 461
ApaLI G'TGCA_C
Cuts at: 0 259 702
Size: 259 443
BanII G_rGCy'C
Cuts at: 0 99 241 270 702
Size: 99 142 29 432
Fragments arranged by size:
432 142 99 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 517 702
Size: 517 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 250 284 702
Size: 250 34 418
Fragments arranged by size:
418 250 34
BglII A'GATC_T
Cuts at: 0 540 702
Size: 540 162
BlpI GC'TnA_GC
Cuts at: 0 323 702
Size: 323 379
Bme1580I G_kGCm'C
Cuts at: 0 241 263 702
Size: 241 22 439
Fragments arranged by size:
439 241 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 680 702
Size: 680 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 476 702
Size: 110 366 226
Fragments arranged by size:
366 226 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 702
Size: 541 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 702
Size: 28 674
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 107 702
Size: 107 595
BsiHKAI G_wGCw'C
Cuts at: 0 263 270 702
Size: 263 7 432
Fragments arranged by size:
432 263 7
Bsp1286I G_dGCh'C
Cuts at: 0 99 241 263 270 702
Size: 99 142 22 7 432
Fragments arranged by size:
432 142 99 22 7
BsrDI GCAATG_nn'
Cuts at: 0 491 645 702
Size: 491 154 57
Fragments arranged by size:
491 154 57
BsrGI T'GTAC_A
Cuts at: 0 183 224 702
Size: 183 41 478
Fragments arranged by size:
478 183 41
BstYI r'GATC_y
Cuts at: 0 447 540 702
Size: 447 93 162
Fragments arranged by size:
447 162 93
BtgI C'CryG_G
Cuts at: 0 104 702
Size: 104 598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 110 145 702
Size: 110 35 557
Fragments arranged by size:
557 110 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 702
Size: 19 683
EcoICRI GAG'CTC
Cuts at: 0 268 702
Size: 268 434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 476 692 702
Size: 110 366 216 10
Fragments arranged by size:
366 216 110 10
EcoO109I rG'GnC_Cy
Cuts at: 0 238 702
Size: 238 464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 702
Size: 609 93
HindIII A'AGCT_T
Cuts at: 0 591 702
Size: 591 111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 609 691 702
Size: 394 215 82 11
Fragments arranged by size:
394 215 82 11
MspA1I CmG'CkG
Cuts at: 0 327 702
Size: 327 375
PspOMI G'GGCC_C
Cuts at: 0 237 702
Size: 237 465
PvuII CAG'CTG
Cuts at: 0 327 702
Size: 327 375
SacI G_AGCT'C
Cuts at: 0 270 702
Size: 270 432
SfcI C'TryA_G
Cuts at: 0 83 317 479 702
Size: 83 234 162 223
Fragments arranged by size:
234 223 162 83
SmlI C'TyrA_G
Cuts at: 0 520 702
Size: 520 182
TatI w'GTAC_w
Cuts at: 0 183 224 702
Size: 183 41 478
Fragments arranged by size:
478 183 41
XmnI GAAnn'nnTTC
Cuts at: 0 311 514 702
Size: 311 203 188
Fragments arranged by size:
311 203 188
Enzymes that do cut:
AcuI ApaI ApaLI BanII BbsI BcgI BglII BlpI
Bme1580I BplI BpmI BpuEI BsaXI BseRI BsiHKAI Bsp1286I
BsrDI BsrGI BstYI BtgI CspCI EciI EcoICRI Eco57MI
EcoO109I FalI HindIII MmeI MspA1I PspOMI PvuII SacI
SfcI SmlI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BciVI BclI BfrBI BglI BmgBI BmrI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI