Restriction map for
pF1KB6952
(Linear) (Six-base) MAPSORT of: KIBB6952.seq Check: 9728 from: 1 to: 705
KIBB6952.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 705
Size: 251 454
AflIII A'CryG_T
Cuts at: 0 568 705
Size: 568 137
ApoI r'AATT_y
Cuts at: 0 319 340 389 421 667 705
Size: 319 21 49 32 246 38
Fragments arranged by size:
319 246 49 38 32 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 182 618 705
Size: 139 43 436 87
Fragments arranged by size:
436 139 87 43
BglII A'GATC_T
Cuts at: 0 193 705
Size: 193 512
Bme1580I G_kGCm'C
Cuts at: 0 203 705
Size: 203 502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 705
Size: 171 534
Bpu10I CC'TnA_GC
Cuts at: 0 122 705
Size: 122 583
BsaI GGTCTCn'nnnn_
Cuts at: 0 615 705
Size: 615 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 491 521 705
Size: 169 30 292 30 184
Fragments arranged by size:
292 184 169 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 509 705
Size: 175 334 196
Fragments arranged by size:
334 196 175
BsmBI CGTCTCn'nnnn_
Cuts at: 0 633 705
Size: 633 72
Bsp1286I G_dGCh'C
Cuts at: 0 203 705
Size: 203 502
BspHI T'CATG_A
Cuts at: 0 171 705
Size: 171 534
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 667 705
Size: 667 38
BsrBI CCG'CTC
Cuts at: 0 185 705
Size: 185 520
BsrDI GCAATG_nn'
Cuts at: 0 10 669 705
Size: 10 659 36
Fragments arranged by size:
659 36 10
BsrGI T'GTAC_A
Cuts at: 0 678 705
Size: 678 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 283 705
Size: 283 422
BstYI r'GATC_y
Cuts at: 0 193 705
Size: 193 512
BtsI GCAGTG_nn'
Cuts at: 0 245 705
Size: 245 460
EaeI y'GGCC_r
Cuts at: 0 206 705
Size: 206 499
EarI CTCTTCn'nnn_
Cuts at: 0 227 705
Size: 227 478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 251 705
Size: 171 80 454
Fragments arranged by size:
454 171 80
EcoO109I rG'GnC_Cy
Cuts at: 0 379 705
Size: 379 326
EcoRI G'AATT_C
Cuts at: 0 319 667 705
Size: 319 348 38
Fragments arranged by size:
348 319 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 667 699 705
Size: 169 32 466 32 6
Fragments arranged by size:
466 169 32 32 6
HincII GTy'rAC
Cuts at: 0 453 705
Size: 453 252
HpaI GTT'AAC
Cuts at: 0 453 705
Size: 453 252
MscI TGG'CCA
Cuts at: 0 208 705
Size: 208 497
MslI CAynn'nnrTG
Cuts at: 0 281 705
Size: 281 424
PpuMI rG'GwC_Cy
Cuts at: 0 379 705
Size: 379 326
SspI AAT'ATT
Cuts at: 0 336 705
Size: 336 369
TatI w'GTAC_w
Cuts at: 0 678 705
Size: 678 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 339 371 705
Size: 339 32 334
Fragments arranged by size:
339 334 32
Tth111I GACn'n_nGTC
Cuts at: 0 143 705
Size: 143 562
Enzymes that do cut:
AcuI AflIII ApoI BbsI BglII Bme1580I BpmI Bpu10I
BsaI BsaXI BsgI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrGI BstXI BstYI BtsI EaeI EarI Eco57MI
EcoO109I EcoRI Hin4I HincII HpaI MscI MslI PpuMI
SspI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BspEI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII XbaI XcmI XhoI XmaI XmnI ZraI