(Linear) (Six-base) MAPSORT of: KIBB6953.seq  Check: 3239  from: 1  to: 636



KIBB6953.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:      0     191     636 
   Size:        191     445
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     636 
   Size:        413     223
 
AfeI AGC'GCT

Cuts at:      0     154     636 
   Size:        154     482
 
ApoI r'AATT_y

Cuts at:      0     285     360     636 
   Size:        285      75     276

  Fragments arranged by size: 

                 285     276      75
 
AvaI C'yCGr_G

Cuts at:      0     229     636 
   Size:        229     407
 
AvrII C'CTAG_G

Cuts at:      0     262     636 
   Size:        262     374
 
BanII G_rGCy'C

Cuts at:      0      54      83     636 
   Size:         54      29     553

  Fragments arranged by size: 

                 553      54      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     401     636 
   Size:        401     235
 
BlpI GC'TnA_GC

Cuts at:      0      77      82     636 
   Size:         77       5     554

  Fragments arranged by size: 

                 554      77       5
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     116     636 
   Size:        116     520
 
BmtI G_CTAG'C

Cuts at:      0     338     636 
   Size:        338     298
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     582     636 
   Size:         38     544      54

  Fragments arranged by size: 

                 544      54      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     532     636 
   Size:        532     104
 
BsiHKAI G_wGCw'C

Cuts at:      0      54      83     236     630     636 
   Size:         54      29     153     394       6

  Fragments arranged by size: 

                 394     153      54      29       6
 
Bsp1286I G_dGCh'C

Cuts at:      0      54      83     236     630     636 
   Size:         54      29     153     394       6

  Fragments arranged by size: 

                 394     153      54      29       6
 
BspHI T'CATG_A

Cuts at:      0      14     570     636 
   Size:         14     556      66

  Fragments arranged by size: 

                 556      66      14
 
BsrFI r'CCGG_y

Cuts at:      0     143     636 
   Size:        143     493
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     130     636 
   Size:        130     506
 
EcoICRI GAG'CTC

Cuts at:      0      52      81     636 
   Size:         52      29     555

  Fragments arranged by size: 

                 555      52      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     413     582     636 
   Size:         38     375     169      54

  Fragments arranged by size: 

                 375     169      54      38
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     294     636 
   Size:        294     342
 
HaeII r_GCGC'y

Cuts at:      0      77     156     636 
   Size:         77      79     480

  Fragments arranged by size: 

                 480      79      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       9      41     636 
   Size:          9      32     595

  Fragments arranged by size: 

                 595      32       9
 
HincII GTy'rAC

Cuts at:      0     222     636 
   Size:        222     414
 
HindIII A'AGCT_T

Cuts at:      0     448     636 
   Size:        448     188
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     515     636 
   Size:        515     121
 
NheI G'CTAG_C

Cuts at:      0     334     636 
   Size:        334     302
 
SacI G_AGCT'C

Cuts at:      0      54      83     636 
   Size:         54      29     553

  Fragments arranged by size: 

                 553      54      29
 
SexAI A'CCwGG_T

Cuts at:      0     529     636 
   Size:        529     107
 
StyI C'CwwG_G

Cuts at:      0      45     262     278     636 
   Size:         45     217      16     358

  Fragments arranged by size: 

                 358     217      45      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      82     636 
   Size:         82     554
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     108     140     169     201     636 
   Size:        108      32      29      32     435

  Fragments arranged by size: 

                 435     108      32      32      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     276     636 
   Size:        276     360
 
XmnI GAAnn'nnTTC

Cuts at:      0     504     636 
   Size:        504     132
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     ApoI     AvaI    AvrII    BanII     BbsI
     BlpI     BmrI     BmtI     BpmI     BsaI  BsiHKAI Bsp1286I    BspHI
    BsrFI     EciI  EcoICRI  Eco57MI     FalI    HaeII    Hin4I   HincII
  HindIII     MmeI     NheI     SacI    SexAI     StyI    TaqII     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     ZraI