(Linear) (Six-base) MAPSORT of: KIBB6956.seq  Check: 2834  from: 1  to: 714



KIBB6956.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     291     714 
   Size:        291     423
 
AatII G_ACGT'C

Cuts at:      0     294     714 
   Size:        294     420
 
ApoI r'AATT_y

Cuts at:      0     157     714 
   Size:        157     557
 
AvaI C'yCGr_G

Cuts at:      0     224     714 
   Size:        224     490
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     234     268     714 
   Size:        234      34     446

  Fragments arranged by size: 

                 446     234      34
 
BglII A'GATC_T

Cuts at:      0     490     532     640     714 
   Size:        490      42     108      74

  Fragments arranged by size: 

                 490     108      74      42
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     152     714 
   Size:        152     562
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     714 
   Size:        122     592
 
BsaBI GATnn'nnATC

Cuts at:      0     356     489     513     594     645     714 
   Size:        356     133      24      81      51      69

  Fragments arranged by size: 

                 356     133      81      69      51      24
 
BsaHI Gr'CG_yC

Cuts at:      0     291     714 
   Size:        291     423
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     252     282     714 
   Size:        252      30     432

  Fragments arranged by size: 

                 432     252      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      38     467     714 
   Size:         38     429     247

  Fragments arranged by size: 

                 429     247      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     291     374     714 
   Size:        291      83     340

  Fragments arranged by size: 

                 340     291      83
 
BsrFI r'CCGG_y

Cuts at:      0     368     714 
   Size:        368     346
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     184     714 
   Size:        184     530
 
BstBI TT'CG_AA

Cuts at:      0     161     714 
   Size:        161     553
 
BstEII G'GTnAC_C

Cuts at:      0      46     714 
   Size:         46     668
 
BstYI r'GATC_y

Cuts at:      0     165     490     532     595     640     714 
   Size:        165     325      42      63      45      74

  Fragments arranged by size: 

                 325     165      74      63      45      42
 
EarI CTCTTCn'nnn_

Cuts at:      0     506     714 
   Size:        506     208
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     714 
   Size:        122     592
 
EcoRI G'AATT_C

Cuts at:      0     157     714 
   Size:        157     557
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     536     714 
   Size:        536     178
 
HaeII r_GCGC'y

Cuts at:      0      65     714 
   Size:         65     649
 
NaeI GCC'GGC

Cuts at:      0     370     714 
   Size:        370     344
 
NgoMIV G'CCGG_C

Cuts at:      0     368     714 
   Size:        368     346
 
NspI r_CATG'y

Cuts at:      0     255     714 
   Size:        255     459
 
PfoI T'CCnGG_A

Cuts at:      0     138     714 
   Size:        138     576
 
PshAI GACnn'nnGTC

Cuts at:      0     459     714 
   Size:        459     255
 
SphI G_CATG'C

Cuts at:      0     255     714 
   Size:        255     459
 
StyI C'CwwG_G

Cuts at:      0      32     714 
   Size:         32     682
 
XbaI T'CTAG_A

Cuts at:      0     394     714 
   Size:        394     320
 
XmnI GAAnn'nnTTC

Cuts at:      0     663     714 
   Size:        663      51
 
ZraI GAC'GTC

Cuts at:      0     292     714 
   Size:        292     422
 

 Enzymes that do cut:

     AarI    AatII     ApoI     AvaI     BcgI    BglII     BplI     BpmI
    BsaBI    BsaHI    BsaXI    BseRI    BspMI    BsrFI   BstAPI    BstBI
   BstEII    BstYI     EarI  Eco57MI    EcoRI     FalI    HaeII     NaeI
   NgoMIV     NspI     PfoI    PshAI     SphI     StyI     XbaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI