(Linear) (Six-base) MAPSORT of: KIBB6961.seq  Check: 3500  from: 1  to: 525



KIBB6961.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

ApoI r'AATT_y

Cuts at:      0     283     525 
   Size:        283     242
 
BanI G'GyrC_C

Cuts at:      0     106     525 
   Size:        106     419
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     217     525 
   Size:        217     308
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     208     525 
   Size:        208     317
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     356     525 
   Size:        105     251     169

  Fragments arranged by size: 

                 251     169     105
 
BsmI GAATG_Cn'

Cuts at:      0      40     525 
   Size:         40     485
 
BstEII G'GTnAC_C

Cuts at:      0      42     525 
   Size:         42     483
 
BtgI C'CryG_G

Cuts at:      0      98     525 
   Size:         98     427
 
ClaI AT'CG_AT

Cuts at:      0     113     525 
   Size:        113     412
 
DraI TTT'AAA

Cuts at:      0      60     525 
   Size:         60     465
 
EarI CTCTTCn'nnn_

Cuts at:      0     326     459     525 
   Size:        326     133      66

  Fragments arranged by size: 

                 326     133      66
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     132     164     204     236     525 
   Size:        132      32      40      32     289

  Fragments arranged by size: 

                 289     132      40      32      32
 
HincII GTy'rAC

Cuts at:      0       8     509     525 
   Size:          8     501      16

  Fragments arranged by size: 

                 501      16       8
 
HpaI GTT'AAC

Cuts at:      0       8     525 
   Size:          8     517
 
MspA1I CmG'CkG

Cuts at:      0      18     525 
   Size:         18     507
 
NcoI C'CATG_G

Cuts at:      0      98     525 
   Size:         98     427
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     210     242     525 
   Size:        210      32     283

  Fragments arranged by size: 

                 283     210      32
 
PvuII CAG'CTG

Cuts at:      0      18     525 
   Size:         18     507
 
SfcI C'TryA_G

Cuts at:      0     134     525 
   Size:        134     391
 
SmlI C'TyrA_G

Cuts at:      0      84     371     525 
   Size:         84     287     154

  Fragments arranged by size: 

                 287     154      84
 
SspI AAT'ATT

Cuts at:      0     264     423     525 
   Size:        264     159     102

  Fragments arranged by size: 

                 264     159     102
 
StyI C'CwwG_G

Cuts at:      0      98     525 
   Size:         98     427
 

 Enzymes that do cut:

     ApoI     BanI     BbsI     BmrI    BpuEI     BsmI   BstEII     BtgI
     ClaI     DraI     EarI    Hin4I   HincII     HpaI   MspA1I     NcoI
     PsrI    PvuII     SfcI     SmlI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI