Restriction map for
pF1KB6962
(Linear) (Six-base) MAPSORT of: KIBB6962.seq Check: 2368 from: 1 to: 720
KIBB6962.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 0 218 492 533 720
Size: 218 274 41 187
Fragments arranged by size:
274 218 187 41
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 679 720
Size: 679 41
AlwNI CAG_nnn'CTG
Cuts at: 0 376 720
Size: 376 344
ApaI G_GGCC'C
Cuts at: 0 484 720
Size: 484 236
BanI G'GyrC_C
Cuts at: 0 70 83 273 424 720
Size: 70 13 190 151 296
Fragments arranged by size:
296 190 151 70 13
BanII G_rGCy'C
Cuts at: 0 484 546 720
Size: 484 62 174
Fragments arranged by size:
484 174 62
BbeI G_GCGC'C
Cuts at: 0 74 277 720
Size: 74 203 443
Fragments arranged by size:
443 203 74
BbvCI CC'TCA_GC
Cuts at: 0 179 720
Size: 179 541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 290 324 614 648 720
Size: 290 34 290 34 72
Fragments arranged by size:
290 290 72 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 68 720
Size: 68 652
Bme1580I G_kGCm'C
Cuts at: 0 88 484 563 720
Size: 88 396 79 157
Fragments arranged by size:
396 157 88 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 716 720
Size: 716 4
BmtI G_CTAG'C
Cuts at: 0 43 720
Size: 43 677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 659 720
Size: 505 154 61
Fragments arranged by size:
505 154 61
Bpu10I CC'TnA_GC
Cuts at: 0 179 720
Size: 179 541
BsaI GGTCTCn'nnnn_
Cuts at: 0 398 677 720
Size: 398 279 43
Fragments arranged by size:
398 279 43
BsaAI yAC'GTr
Cuts at: 0 558 720
Size: 558 162
BsaHI Gr'CG_yC
Cuts at: 0 71 274 720
Size: 71 203 446
Fragments arranged by size:
446 203 71
BsaWI w'CCGG_w
Cuts at: 0 587 720
Size: 587 133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 154 355 597 684 720
Size: 151 3 201 242 87 36
Fragments arranged by size:
242 201 151 87 36 3
BseYI C'CCAG_C
Cuts at: 0 443 720
Size: 443 277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 720
Size: 127 593
BsiEI CG_ry'CG
Cuts at: 0 300 720
Size: 300 420
Bsp1286I G_dGCh'C
Cuts at: 0 88 484 546 563 720
Size: 88 396 62 17 157
Fragments arranged by size:
396 157 88 62 17
BsrBI CCG'CTC
Cuts at: 0 605 720
Size: 605 115
BsrDI GCAATG_nn'
Cuts at: 0 690 720
Size: 690 30
BssHII G'CGCG_C
Cuts at: 0 323 720
Size: 323 397
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 376 720
Size: 105 271 344
Fragments arranged by size:
344 271 105
Bsu36I CC'TnA_GG
Cuts at: 0 350 720
Size: 350 370
BtsI GCAGTG_nn'
Cuts at: 0 253 374 604 720
Size: 253 121 230 116
Fragments arranged by size:
253 230 121 116
EaeI y'GGCC_r
Cuts at: 0 297 720
Size: 297 423
EagI C'GGCC_G
Cuts at: 0 297 720
Size: 297 423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 245 720
Size: 245 475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 659 720
Size: 505 154 61
Fragments arranged by size:
505 154 61
EcoO109I rG'GnC_Cy
Cuts at: 0 628 720
Size: 628 92
HaeII r_GCGC'y
Cuts at: 0 74 277 289 624 720
Size: 74 203 12 335 96
Fragments arranged by size:
335 203 96 74 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 720
Size: 136 32 552
Fragments arranged by size:
552 136 32
KasI G'GCGC_C
Cuts at: 0 70 273 720
Size: 70 203 447
Fragments arranged by size:
447 203 70
MfeI C'AATT_G
Cuts at: 0 564 720
Size: 564 156
MslI CAynn'nnrTG
Cuts at: 0 475 720
Size: 475 245
MspA1I CmG'CkG
Cuts at: 0 27 308 720
Size: 27 281 412
Fragments arranged by size:
412 281 27
NarI GG'CG_CC
Cuts at: 0 71 274 720
Size: 71 203 446
Fragments arranged by size:
446 203 71
NheI G'CTAG_C
Cuts at: 0 39 720
Size: 39 681
PasI CC'CwG_GG
Cuts at: 0 612 720
Size: 612 108
PpuMI rG'GwC_Cy
Cuts at: 0 628 720
Size: 628 92
PshAI GACnn'nnGTC
Cuts at: 0 529 720
Size: 529 191
PspOMI G'GGCC_C
Cuts at: 0 480 720
Size: 480 240
PstI C_TGCA'G
Cuts at: 0 248 515 720
Size: 248 267 205
Fragments arranged by size:
267 248 205
SfcI C'TryA_G
Cuts at: 0 244 511 720
Size: 244 267 209
Fragments arranged by size:
267 244 209
SfoI GGC'GCC
Cuts at: 0 72 275 720
Size: 72 203 445
Fragments arranged by size:
445 203 72
StyI C'CwwG_G
Cuts at: 0 74 260 720
Size: 74 186 460
Fragments arranged by size:
460 186 74
Enzymes that do cut:
AccI AlfI AlwNI ApaI BanI BanII BbeI BbvCI
BcgI BglI Bme1580I BmrI BmtI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI Bsp1286I
BsrBI BsrDI BssHII BstAPI Bsu36I BtsI EaeI EagI
EciI Eco57MI EcoO109I HaeII Hin4I KasI MfeI MslI
MspA1I NarI NheI PasI PpuMI PshAI PspOMI PstI
SfcI SfoI StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbsI BciVI BclI
BfrBI BglII BlpI BmgBI BplI BpuEI BsaBI BsaXI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI
BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MluI MmeI MscI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI