(Linear) (Six-base) MAPSORT of: KIBB6964.seq  Check: 4812  from: 1  to: 723



KIBB6964.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:      0     361     446     723 
   Size:        361      85     277

  Fragments arranged by size: 

                 361     277      85
 
AflII C'TTAA_G

Cuts at:      0     711     723 
   Size:        711      12
 
AlwNI CAG_nnn'CTG

Cuts at:      0     525     723 
   Size:        525     198
 
ApaI G_GGCC'C

Cuts at:      0      73     723 
   Size:         73     650
 
BanII G_rGCy'C

Cuts at:      0      73     723 
   Size:         73     650
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     609     723 
   Size:        609     114
 
BlpI GC'TnA_GC

Cuts at:      0     501     723 
   Size:        501     222
 
Bme1580I G_kGCm'C

Cuts at:      0      73     723 
   Size:         73     650
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     659     723 
   Size:        659      64
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     515     723 
   Size:        152     363     208

  Fragments arranged by size: 

                 363     208     152
 
BsaWI w'CCGG_w

Cuts at:      0     662     723 
   Size:        662      61
 
BsmI GAATG_Cn'

Cuts at:      0     312     723 
   Size:        312     411
 
Bsp1286I G_dGCh'C

Cuts at:      0      73     723 
   Size:         73     650
 
BspEI T'CCGG_A

Cuts at:      0     662     723 
   Size:        662      61
 
BsrDI GCAATG_nn'

Cuts at:      0     359     723 
   Size:        359     364
 
BsrGI T'GTAC_A

Cuts at:      0     644     723 
   Size:        644      79
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     151     349     723 
   Size:        151     198     374

  Fragments arranged by size: 

                 374     198     151
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     603     723 
   Size:        603     120
 
BstYI r'GATC_y

Cuts at:      0     665     723 
   Size:        665      58
 
BstZ17I GTA'TAC

Cuts at:      0     447     723 
   Size:        447     276
 
BtgI C'CryG_G

Cuts at:      0      65     723 
   Size:         65     658
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     232     723 
   Size:        232     491
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     515     723 
   Size:        152     363     208

  Fragments arranged by size: 

                 363     208     152
 
EcoO109I rG'GnC_Cy

Cuts at:      0      70     723 
   Size:         70     653
 
HaeII r_GCGC'y

Cuts at:      0     384     723 
   Size:        384     339
 
MfeI C'AATT_G

Cuts at:      0     125     146     344     723 
   Size:        125      21     198     379

  Fragments arranged by size: 

                 379     198     125      21
 
MspA1I CmG'CkG

Cuts at:      0      67      81     248     579     723 
   Size:         67      14     167     331     144

  Fragments arranged by size: 

                 331     167     144      67      14
 
NspI r_CATG'y

Cuts at:      0     558     574     723 
   Size:        558      16     149

  Fragments arranged by size: 

                 558     149      16
 
PspOMI G'GGCC_C

Cuts at:      0      69     723 
   Size:         69     654
 
PvuII CAG'CTG

Cuts at:      0      81     723 
   Size:         81     642
 
SacII CC_GC'GG

Cuts at:      0      68     723 
   Size:         68     655
 
SexAI A'CCwGG_T

Cuts at:      0     536     723 
   Size:        536     187
 
SfcI C'TryA_G

Cuts at:      0      11     517     723 
   Size:         11     506     206

  Fragments arranged by size: 

                 506     206      11
 
SmlI C'TyrA_G

Cuts at:      0     711     723 
   Size:        711      12
 
StyI C'CwwG_G

Cuts at:      0     584     723 
   Size:        584     139
 
TatI w'GTAC_w

Cuts at:      0     644     723 
   Size:        644      79
 

 Enzymes that do cut:

     AccI    AflII    AlwNI     ApaI    BanII     BbsI     BlpI Bme1580I
     BmrI     BpmI    BsaWI     BsmI Bsp1286I    BspEI    BsrDI    BsrGI
   BstAPI    BstXI    BstYI  BstZ17I     BtgI     DrdI  Eco57MI EcoO109I
    HaeII     MfeI   MspA1I     NspI   PspOMI    PvuII    SacII    SexAI
     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI