Restriction map for
pF1KB6965
(Linear) (Six-base) MAPSORT of: KIBB6965.seq Check: 6792 from: 1 to: 519
KIBB6965.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 0 508 519
Size: 508 11
AflIII A'CryG_T
Cuts at: 0 470 519
Size: 470 49
AlwNI CAG_nnn'CTG
Cuts at: 0 123 519
Size: 123 396
AvaI C'yCGr_G
Cuts at: 0 191 519
Size: 191 328
BanI G'GyrC_C
Cuts at: 0 500 519
Size: 500 19
BbeI G_GCGC'C
Cuts at: 0 504 519
Size: 504 15
BglI GCCn_nnn'nGGC
Cuts at: 0 193 519
Size: 193 326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 519
Size: 380 139
BsaHI Gr'CG_yC
Cuts at: 0 501 519
Size: 501 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 353 383 519
Size: 353 30 136
Fragments arranged by size:
353 136 30
BseYI C'CCAG_C
Cuts at: 0 428 519
Size: 428 91
BsiHKAI G_wGCw'C
Cuts at: 0 443 519
Size: 443 76
BsmI GAATG_Cn'
Cuts at: 0 189 499 519
Size: 189 310 20
Fragments arranged by size:
310 189 20
Bsp1286I G_dGCh'C
Cuts at: 0 443 519
Size: 443 76
BspHI T'CATG_A
Cuts at: 0 200 443 519
Size: 200 243 76
Fragments arranged by size:
243 200 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 519
Size: 164 355
BsrFI r'CCGG_y
Cuts at: 0 92 497 519
Size: 92 405 22
Fragments arranged by size:
405 92 22
BsrGI T'GTAC_A
Cuts at: 0 2 519
Size: 2 517
BstAPI GCAn_nnn'nTGC
Cuts at: 0 149 519
Size: 149 370
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 519
Size: 54 465
BtgI C'CryG_G
Cuts at: 0 65 519
Size: 65 454
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 156 519
Size: 156 363
BtsI GCAGTG_nn'
Cuts at: 0 443 519
Size: 443 76
EaeI y'GGCC_r
Cuts at: 0 62 347 519
Size: 62 285 172
Fragments arranged by size:
285 172 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 519
Size: 380 139
EcoO109I rG'GnC_Cy
Cuts at: 0 104 120 519
Size: 104 16 399
Fragments arranged by size:
399 104 16
HaeII r_GCGC'y
Cuts at: 0 405 504 519
Size: 405 99 15
Fragments arranged by size:
405 99 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 353 385 519
Size: 353 32 134
Fragments arranged by size:
353 134 32
KasI G'GCGC_C
Cuts at: 0 500 519
Size: 500 19
MscI TGG'CCA
Cuts at: 0 64 349 519
Size: 64 285 170
Fragments arranged by size:
285 170 64
MslI CAynn'nnrTG
Cuts at: 0 238 519
Size: 238 281
NaeI GCC'GGC
Cuts at: 0 499 519
Size: 499 20
NarI GG'CG_CC
Cuts at: 0 501 519
Size: 501 18
NcoI C'CATG_G
Cuts at: 0 65 519
Size: 65 454
NgoMIV G'CCGG_C
Cuts at: 0 497 519
Size: 497 22
NspI r_CATG'y
Cuts at: 0 474 519
Size: 474 45
PciI A'CATG_T
Cuts at: 0 470 519
Size: 470 49
PpuMI rG'GwC_Cy
Cuts at: 0 104 519
Size: 104 415
PstI C_TGCA'G
Cuts at: 0 161 519
Size: 161 358
SfcI C'TryA_G
Cuts at: 0 157 519
Size: 157 362
SfoI GGC'GCC
Cuts at: 0 502 519
Size: 502 17
SmaI CCC'GGG
Cuts at: 0 193 519
Size: 193 326
StyI C'CwwG_G
Cuts at: 0 65 344 519
Size: 65 279 175
Fragments arranged by size:
279 175 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4 27 420 519
Size: 4 23 393 99
Fragments arranged by size:
393 99 23 4
TatI w'GTAC_w
Cuts at: 0 2 519
Size: 2 517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 519
Size: 54 465
XmaI C'CCGG_G
Cuts at: 0 191 519
Size: 191 328
Enzymes that do cut:
AccI AflIII AlwNI AvaI BanI BbeI BglI BpmI
BsaHI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrFI BsrGI BstAPI BstXI BtgI BtgZI BtsI EaeI
Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI NaeI
NarI NcoI NgoMIV NspI PciI PpuMI PstI SfcI
SfoI SmaI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MspA1I
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI