Restriction map for
pF1KB6969
(Linear) (Six-base) MAPSORT of: KIBB6969.seq Check: 2766 from: 1 to: 648
KIBB6969.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 43 648
Size: 43 605
ApaI G_GGCC'C
Cuts at: 0 282 648
Size: 282 366
ApoI r'AATT_y
Cuts at: 0 433 648
Size: 433 215
BanII G_rGCy'C
Cuts at: 0 282 560 648
Size: 282 278 88
Fragments arranged by size:
282 278 88
BglII A'GATC_T
Cuts at: 0 53 218 648
Size: 53 165 430
Fragments arranged by size:
430 165 53
Bme1580I G_kGCm'C
Cuts at: 0 282 648
Size: 282 366
BmrI ACTGGGnnnn_n'
Cuts at: 0 351 648
Size: 351 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 589 648
Size: 563 26 59
Fragments arranged by size:
563 59 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 561 591 648
Size: 561 30 57
Fragments arranged by size:
561 57 30
BseYI C'CCAG_C
Cuts at: 0 43 78 618 648
Size: 43 35 540 30
Fragments arranged by size:
540 43 35 30
BsiHKAI G_wGCw'C
Cuts at: 0 157 579 648
Size: 157 422 69
Fragments arranged by size:
422 157 69
BsmI GAATG_Cn'
Cuts at: 0 570 648
Size: 570 78
Bsp1286I G_dGCh'C
Cuts at: 0 157 282 560 579 648
Size: 157 125 278 19 69
Fragments arranged by size:
278 157 125 69 19
BsrBI CCG'CTC
Cuts at: 0 166 243 648
Size: 166 77 405
Fragments arranged by size:
405 166 77
BsrDI GCAATG_nn'
Cuts at: 0 383 509 648
Size: 383 126 139
Fragments arranged by size:
383 139 126
BssSI C'ACGA_G
Cuts at: 0 139 648
Size: 139 509
BstAPI GCAn_nnn'nTGC
Cuts at: 0 499 574 648
Size: 499 75 74
Fragments arranged by size:
499 75 74
BstYI r'GATC_y
Cuts at: 0 53 218 648
Size: 53 165 430
Fragments arranged by size:
430 165 53
BtgI C'CryG_G
Cuts at: 0 428 648
Size: 428 220
DrdI GACnn_nn'nnGTC
Cuts at: 0 205 648
Size: 205 443
EaeI y'GGCC_r
Cuts at: 0 413 648
Size: 413 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 589 648
Size: 563 26 59
Fragments arranged by size:
563 59 26
EcoO109I rG'GnC_Cy
Cuts at: 0 278 648
Size: 278 370
EcoRI G'AATT_C
Cuts at: 0 433 648
Size: 433 215
HindIII A'AGCT_T
Cuts at: 0 541 648
Size: 541 107
MfeI C'AATT_G
Cuts at: 0 158 648
Size: 158 490
MscI TGG'CCA
Cuts at: 0 415 648
Size: 415 233
MspA1I CmG'CkG
Cuts at: 0 40 232 648
Size: 40 192 416
Fragments arranged by size:
416 192 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 43 341 648
Size: 43 298 307
Fragments arranged by size:
307 298 43
PfoI T'CCnGG_A
Cuts at: 0 437 605 648
Size: 437 168 43
Fragments arranged by size:
437 168 43
PspOMI G'GGCC_C
Cuts at: 0 278 648
Size: 278 370
PstI C_TGCA'G
Cuts at: 0 643 648
Size: 643 5
PvuII CAG'CTG
Cuts at: 0 40 232 648
Size: 40 192 416
Fragments arranged by size:
416 192 40
SfcI C'TryA_G
Cuts at: 0 355 639 648
Size: 355 284 9
Fragments arranged by size:
355 284 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 648
Size: 333 315
TatI w'GTAC_w
Cuts at: 0 266 648
Size: 266 382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 568 648
Size: 568 80
Enzymes that do cut:
AlwNI ApaI ApoI BanII BglII Bme1580I BmrI BpmI
BsaXI BseYI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BssSI
BstAPI BstYI BtgI DrdI EaeI Eco57MI EcoO109I EcoRI
HindIII MfeI MscI MspA1I PflMI PfoI PspOMI PstI
PvuII SfcI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrFI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI