Restriction map for
pF1KB6970
(Linear) (Six-base) MAPSORT of: KIBB6970.seq Check: 6085 from: 1 to: 723
KIBB6970.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 723
Size: 503 220
AflIII A'CryG_T
Cuts at: 0 680 723
Size: 680 43
ApoI r'AATT_y
Cuts at: 0 672 723
Size: 672 51
AvaI C'yCGr_G
Cuts at: 0 500 723
Size: 500 223
BamHI G'GATC_C
Cuts at: 0 116 723
Size: 116 607
BanI G'GyrC_C
Cuts at: 0 391 526 723
Size: 391 135 197
Fragments arranged by size:
391 197 135
BanII G_rGCy'C
Cuts at: 0 182 538 723
Size: 182 356 185
Fragments arranged by size:
356 185 182
BbsI GAAGACnn'nnnn_
Cuts at: 0 581 723
Size: 581 142
BglI GCCn_nnn'nGGC
Cuts at: 0 85 723
Size: 85 638
Bme1580I G_kGCm'C
Cuts at: 0 531 723
Size: 531 192
BmtI G_CTAG'C
Cuts at: 0 652 723
Size: 652 71
BsaBI GATnn'nnATC
Cuts at: 0 383 723
Size: 383 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 30 60 723
Size: 30 30 663
Fragments arranged by size:
663 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 446 634 723
Size: 446 188 89
Fragments arranged by size:
446 188 89
BseYI C'CCAG_C
Cuts at: 0 181 452 723
Size: 181 271 271
Fragments arranged by size:
271 271 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 723
Size: 679 44
BsiHKAI G_wGCw'C
Cuts at: 0 538 723
Size: 538 185
BsmI GAATG_Cn'
Cuts at: 0 636 723
Size: 636 87
Bsp1286I G_dGCh'C
Cuts at: 0 182 531 538 723
Size: 182 349 7 185
Fragments arranged by size:
349 185 182 7
BsrGI T'GTAC_A
Cuts at: 0 486 723
Size: 486 237
BstYI r'GATC_y
Cuts at: 0 116 384 723
Size: 116 268 339
Fragments arranged by size:
339 268 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 305 340 723
Size: 305 35 383
Fragments arranged by size:
383 305 35
EaeI y'GGCC_r
Cuts at: 0 666 723
Size: 666 57
EarI CTCTTCn'nnn_
Cuts at: 0 148 698 723
Size: 148 550 25
Fragments arranged by size:
550 148 25
EcoICRI GAG'CTC
Cuts at: 0 536 723
Size: 536 187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 723
Size: 503 220
EcoO109I rG'GnC_Cy
Cuts at: 0 448 479 723
Size: 448 31 244
Fragments arranged by size:
448 244 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 198 230 723
Size: 198 32 493
Fragments arranged by size:
493 198 32
HindIII A'AGCT_T
Cuts at: 0 94 723
Size: 94 629
MfeI C'AATT_G
Cuts at: 0 506 723
Size: 506 217
MscI TGG'CCA
Cuts at: 0 668 723
Size: 668 55
MspA1I CmG'CkG
Cuts at: 0 456 723
Size: 456 267
NdeI CA'TA_TG
Cuts at: 0 464 723
Size: 464 259
NheI G'CTAG_C
Cuts at: 0 648 723
Size: 648 75
NspI r_CATG'y
Cuts at: 0 684 723
Size: 684 39
PciI A'CATG_T
Cuts at: 0 680 723
Size: 680 43
PpuMI rG'GwC_Cy
Cuts at: 0 448 479 723
Size: 448 31 244
Fragments arranged by size:
448 244 31
PspXI vC'TCGA_Gb
Cuts at: 0 500 723
Size: 500 223
PvuII CAG'CTG
Cuts at: 0 456 723
Size: 456 267
SacI G_AGCT'C
Cuts at: 0 538 723
Size: 538 185
SfcI C'TryA_G
Cuts at: 0 104 723
Size: 104 619
SmlI C'TyrA_G
Cuts at: 0 500 723
Size: 500 223
TatI w'GTAC_w
Cuts at: 0 486 723
Size: 486 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 584 616 723
Size: 584 32 107
Fragments arranged by size:
584 107 32
XhoI C'TCGA_G
Cuts at: 0 500 723
Size: 500 223
XmnI GAAnn'nnTTC
Cuts at: 0 162 723
Size: 162 561
Enzymes that do cut:
AcuI AflIII ApoI AvaI BamHI BanI BanII BbsI
BglI Bme1580I BmtI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BsrGI BstYI CspCI EaeI EarI
EcoICRI Eco57MI EcoO109I Hin4I HindIII MfeI MscI MspA1I
NdeI NheI NspI PciI PpuMI PspXI PvuII SacI
SfcI SmlI TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI MmeI MslI NaeI
NarI NcoI NgoMIV NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII Tth111I XbaI XcmI XmaI ZraI