(Linear) (Six-base) MAPSORT of: KIBB6971.seq  Check: 2665  from: 1  to: 648



KIBB6971.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     601     648 
   Size:        409     192      47

  Fragments arranged by size: 

                 409     192      47
 
AfeI AGC'GCT

Cuts at:      0     364     648 
   Size:        364     284
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     188     220     648 
   Size:        188      32     428

  Fragments arranged by size: 

                 428     188      32
 
ApaI G_GGCC'C

Cuts at:      0     119     648 
   Size:        119     529
 
ApoI r'AATT_y

Cuts at:      0     274     648 
   Size:        274     374
 
BanII G_rGCy'C

Cuts at:      0     119     648 
   Size:        119     529
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      57     648 
   Size:         57     591
 
BclI T'GATC_A

Cuts at:      0     590     648 
   Size:        590      58
 
Bme1580I G_kGCm'C

Cuts at:      0     119     648 
   Size:        119     529
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     444     648 
   Size:        444     204
 
Bpu10I CC'TnA_GC

Cuts at:      0     234     648 
   Size:        234     414
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     193     648 
   Size:        193     455
 
BseYI C'CCAG_C

Cuts at:      0     407     648 
   Size:        407     241
 
BsiEI CG_ry'CG

Cuts at:      0      98     648 
   Size:         98     550
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     553     648 
   Size:         35     518      95

  Fragments arranged by size: 

                 518      95      35
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     119     553     648 
   Size:         35      84     434      95

  Fragments arranged by size: 

                 434      95      84      35
 
BspHI T'CATG_A

Cuts at:      0     299     648 
   Size:        299     349
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      36     579     621     648 
   Size:         36     543      42      27

  Fragments arranged by size: 

                 543      42      36      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     197     232     648 
   Size:        197      35     416

  Fragments arranged by size: 

                 416     197      35
 
EaeI y'GGCC_r

Cuts at:      0      95     419     648 
   Size:         95     324     229

  Fragments arranged by size: 

                 324     229      95
 
EagI C'GGCC_G

Cuts at:      0      95     648 
   Size:         95     553
 
EarI CTCTTCn'nnn_

Cuts at:      0     196     648 
   Size:        196     452
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     326     648 
   Size:        326     322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     601     648 
   Size:        409     192      47

  Fragments arranged by size: 

                 409     192      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0      85     115     648 
   Size:         85      30     533

  Fragments arranged by size: 

                 533      85      30
 
HaeII r_GCGC'y

Cuts at:      0     366     648 
   Size:        366     282
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     171     203     648 
   Size:        171      32     445

  Fragments arranged by size: 

                 445     171      32
 
MscI TGG'CCA

Cuts at:      0     421     648 
   Size:        421     227
 
MslI CAynn'nnrTG

Cuts at:      0      28     529     648 
   Size:         28     501     119

  Fragments arranged by size: 

                 501     119      28
 
NspI r_CATG'y

Cuts at:      0     499     648 
   Size:        499     149
 
PpuMI rG'GwC_Cy

Cuts at:      0      85     648 
   Size:         85     563
 
PspOMI G'GGCC_C

Cuts at:      0     115     648 
   Size:        115     533
 
SfcI C'TryA_G

Cuts at:      0     239     648 
   Size:        239     409
 
StyI C'CwwG_G

Cuts at:      0     641     648 
   Size:        641       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     399     648 
   Size:        399     249
 
XbaI T'CTAG_A

Cuts at:      0     516     648 
   Size:        516     132
 

 Enzymes that do cut:

     AcuI     AfeI     AloI     ApaI     ApoI    BanII     BbsI     BclI
 Bme1580I     BmrI   Bpu10I     BsaI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BtgZI    CspCI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    HaeII    Hin4I     MscI     MslI     NspI    PpuMI   PspOMI
     SfcI     StyI    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI     DraI   DraIII
     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI