(Linear) (Six-base) MAPSORT of: KIBB6978.seq  Check: 4348  from: 1  to: 735



KIBB6978.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     658     735 
   Size:        658      77
 
AflIII A'CryG_T

Cuts at:      0     562     735 
   Size:        562     173
 
AlwNI CAG_nnn'CTG

Cuts at:      0     263     735 
   Size:        263     472
 
ApoI r'AATT_y

Cuts at:      0     280     495     615     661     735 
   Size:        280     215     120      46      74

  Fragments arranged by size: 

                 280     215     120      74      46
 
BglII A'GATC_T

Cuts at:      0     365     735 
   Size:        365     370
 
BmgBI CAC'GTC

Cuts at:      0      26     735 
   Size:         26     709
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      30     735 
   Size:         30     705
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     735 
   Size:        356     379
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     650     680     735 
   Size:        650      30      55

  Fragments arranged by size: 

                 650      55      30
 
BsiHKAI G_wGCw'C

Cuts at:      0      74     156     735 
   Size:         74      82     579

  Fragments arranged by size: 

                 579      82      74
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     156     735 
   Size:         74      82     579

  Fragments arranged by size: 

                 579      82      74
 
BsrDI GCAATG_nn'

Cuts at:      0     143     735 
   Size:        143     592
 
BstYI r'GATC_y

Cuts at:      0     365     594     735 
   Size:        365     229     141

  Fragments arranged by size: 

                 365     229     141
 
BtgI C'CryG_G

Cuts at:      0      92     735 
   Size:         92     643
 
EaeI y'GGCC_r

Cuts at:      0       2      20     735 
   Size:          2      18     715

  Fragments arranged by size: 

                 715      18       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     183     587     735 
   Size:        183     404     148

  Fragments arranged by size: 

                 404     183     148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     658     735 
   Size:        658      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0       9     735 
   Size:          9     726
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     186     218     735 
   Size:        186      32     517

  Fragments arranged by size: 

                 517     186      32
 
NspI r_CATG'y

Cuts at:      0     203     566     735 
   Size:        203     363     169

  Fragments arranged by size: 

                 363     203     169
 
PciI A'CATG_T

Cuts at:      0     562     735 
   Size:        562     173
 
PpuMI rG'GwC_Cy

Cuts at:      0       9     735 
   Size:          9     726
 
PstI C_TGCA'G

Cuts at:      0     259     735 
   Size:        259     476
 
SfcI C'TryA_G

Cuts at:      0     114     255     735 
   Size:        114     141     480

  Fragments arranged by size: 

                 480     141     114
 
SmlI C'TyrA_G

Cuts at:      0     371     735 
   Size:        371     364
 
StyI C'CwwG_G

Cuts at:      0     586     735 
   Size:        586     149
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     650     682     735 
   Size:        650      32      53

  Fragments arranged by size: 

                 650      53      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     719     735 
   Size:        719      16
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI    BglII    BmgBI     BplI    BpuEI
    BsaXI  BsiHKAI Bsp1286I    BsrDI    BstYI     BtgI     EaeI     EarI
  Eco57MI EcoO109I    Hin4I     NspI     PciI    PpuMI     PstI     SfcI
     SmlI     StyI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI