Restriction map for
pF1KB6980
(Linear) (Six-base) MAPSORT of: KIBB6980.seq Check: 1563 from: 1 to: 735
KIBB6980.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 366 735
Size: 366 369
AccI GT'mk_AC
Cuts at: 0 155 735
Size: 155 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 735
Size: 225 510
AhdI GACnn_n'nnGTC
Cuts at: 0 282 735
Size: 282 453
ApaI G_GGCC'C
Cuts at: 0 210 735
Size: 210 525
ApaLI G'TGCA_C
Cuts at: 0 670 735
Size: 670 65
AscI GG'CGCG_CC
Cuts at: 0 51 735
Size: 51 684
AvaI C'yCGr_G
Cuts at: 0 182 188 662 735
Size: 182 6 474 73
Fragments arranged by size:
474 182 73 6
BanII G_rGCy'C
Cuts at: 0 17 181 189 195 210 735
Size: 17 164 8 6 15 525
Fragments arranged by size:
525 164 17 15 8 6
BbvCI CC'TCA_GC
Cuts at: 0 517 735
Size: 517 218
BciVI GTATCCnnnnn_n'
Cuts at: 0 443 735
Size: 443 292
BglI GCCn_nnn'nGGC
Cuts at: 0 430 735
Size: 430 305
BlpI GC'TnA_GC
Cuts at: 0 480 735
Size: 480 255
Bme1580I G_kGCm'C
Cuts at: 0 210 674 735
Size: 210 464 61
Fragments arranged by size:
464 210 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 649 735
Size: 31 618 86
Fragments arranged by size:
618 86 31
Bpu10I CC'TnA_GC
Cuts at: 0 517 735
Size: 517 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 735
Size: 616 119
BsaBI GATnn'nnATC
Cuts at: 0 364 735
Size: 364 371
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 337 367 622 652 735
Size: 4 30 303 30 255 30 83
Fragments arranged by size:
303 255 83 30 30 30 4
BseYI C'CCAG_C
Cuts at: 0 548 726 735
Size: 548 178 9
Fragments arranged by size:
548 178 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 394 598 735
Size: 328 66 204 137
Fragments arranged by size:
328 204 137 66
BsiHKAI G_wGCw'C
Cuts at: 0 17 481 674 735
Size: 17 464 193 61
Fragments arranged by size:
464 193 61 17
BsmI GAATG_Cn'
Cuts at: 0 423 735
Size: 423 312
Bsp1286I G_dGCh'C
Cuts at: 0 17 181 189 195 210 481 674 735
Size: 17 164 8 6 15 271 193 61
Fragments arranged by size:
271 193 164 61 17 15 8 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 366 735
Size: 366 369
BsrBI CCG'CTC
Cuts at: 0 633 735
Size: 633 102
BssHII G'CGCG_C
Cuts at: 0 51 735
Size: 51 684
BssSI C'ACGA_G
Cuts at: 0 166 616 735
Size: 166 450 119
Fragments arranged by size:
450 166 119
BstAPI GCAn_nnn'nTGC
Cuts at: 0 705 735
Size: 705 30
BstBI TT'CG_AA
Cuts at: 0 320 735
Size: 320 415
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 735
Size: 144 591
BstYI r'GATC_y
Cuts at: 0 71 170 735
Size: 71 99 565
Fragments arranged by size:
565 99 71
Bsu36I CC'TnA_GG
Cuts at: 0 576 735
Size: 576 159
BtsI GCAGTG_nn'
Cuts at: 0 440 735
Size: 440 295
EaeI y'GGCC_r
Cuts at: 0 265 735
Size: 265 470
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 298 526 735
Size: 298 228 209
Fragments arranged by size:
298 228 209
EcoICRI GAG'CTC
Cuts at: 0 15 735
Size: 15 720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 225 649 735
Size: 31 194 424 86
Fragments arranged by size:
424 194 86 31
EcoO109I rG'GnC_Cy
Cuts at: 0 206 207 398 735
Size: 206 1 191 337
Fragments arranged by size:
337 206 191 1
EcoRV GAT'ATC
Cuts at: 0 366 735
Size: 366 369
FspI TGC'GCA
Cuts at: 0 67 735
Size: 67 668
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 268 300 735
Size: 4 32 232 32 435
Fragments arranged by size:
435 232 32 32 4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 620 735
Size: 2 618 115
Fragments arranged by size:
618 115 2
MslI CAynn'nnrTG
Cuts at: 0 707 735
Size: 707 28
MspA1I CmG'CkG
Cuts at: 0 342 484 538 552 735
Size: 342 142 54 14 183
Fragments arranged by size:
342 183 142 54 14
NspI r_CATG'y
Cuts at: 0 706 735
Size: 706 29
PpuMI rG'GwC_Cy
Cuts at: 0 398 735
Size: 398 337
PspOMI G'GGCC_C
Cuts at: 0 206 735
Size: 206 529
PspXI vC'TCGA_Gb
Cuts at: 0 188 735
Size: 188 547
PstI C_TGCA'G
Cuts at: 0 305 735
Size: 305 430
PvuII CAG'CTG
Cuts at: 0 342 484 538 552 735
Size: 342 142 54 14 183
Fragments arranged by size:
342 183 142 54 14
SacI G_AGCT'C
Cuts at: 0 17 735
Size: 17 718
SfcI C'TryA_G
Cuts at: 0 301 382 735
Size: 301 81 353
Fragments arranged by size:
353 301 81
SmaI CCC'GGG
Cuts at: 0 184 735
Size: 184 551
SmlI C'TyrA_G
Cuts at: 0 188 595 735
Size: 188 407 140
Fragments arranged by size:
407 188 140
StuI AGG'CCT
Cuts at: 0 715 735
Size: 715 20
StyI C'CwwG_G
Cuts at: 0 137 735
Size: 137 598
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 362 735
Size: 362 373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 735
Size: 337 32 366
Fragments arranged by size:
366 337 32
XhoI C'TCGA_G
Cuts at: 0 188 735
Size: 188 547
XmaI C'CCGG_G
Cuts at: 0 182 735
Size: 182 553
Enzymes that do cut:
AarI AccI AcuI AhdI ApaI ApaLI AscI AvaI
BanII BbvCI BciVI BglI BlpI Bme1580I BpmI Bpu10I
BpuEI BsaBI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtsI EaeI EciI EcoICRI Eco57MI EcoO109I EcoRV
FspI Hin4I MmeI MslI MspA1I NspI PpuMI PspOMI
PspXI PstI PvuII SacI SfcI SmaI SmlI StuI
StyI TaqII TstI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApoI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BclI BfrBI BglII
BmgBI BmrI BmtI BplI BsaI BsaAI BsaHI BsaWI
BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoNI EcoRI FalI FseI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XmnI ZraI