(Linear) (Six-base) MAPSORT of: KIBB6982.seq  Check: 6789  from: 1  to: 738



KIBB6982.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AccI GT'mk_AC

Cuts at:      0     371     607     738 
   Size:        371     236     131

  Fragments arranged by size: 

                 371     236     131
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     738 
   Size:        620     118
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     615     738 
   Size:        615     123
 
AlwNI CAG_nnn'CTG

Cuts at:      0     121     738 
   Size:        121     617
 
ApoI r'AATT_y

Cuts at:      0     556     676     738 
   Size:        556     120      62

  Fragments arranged by size: 

                 556     120      62
 
AvaI C'yCGr_G

Cuts at:      0      52     738 
   Size:         52     686
 
Bme1580I G_kGCm'C

Cuts at:      0     282     738 
   Size:        282     456
 
BsaWI w'CCGG_w

Cuts at:      0     241     738 
   Size:        241     497
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     738 
   Size:        161     577
 
BsmI GAATG_Cn'

Cuts at:      0     387     738 
   Size:        387     351
 
Bsp1286I G_dGCh'C

Cuts at:      0     282     738 
   Size:        282     456
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     460     738 
   Size:        460     278
 
BsrFI r'CCGG_y

Cuts at:      0      19     738 
   Size:         19     719
 
BstYI r'GATC_y

Cuts at:      0     262     738 
   Size:        262     476
 
Bsu36I CC'TnA_GG

Cuts at:      0     446     738 
   Size:        446     292
 
BtgI C'CryG_G

Cuts at:      0       6     738 
   Size:          6     732
 
BtsI GCAGTG_nn'

Cuts at:      0     494     738 
   Size:        494     244
 
DraI TTT'AAA

Cuts at:      0     489     738 
   Size:        489     249
 
EarI CTCTTCn'nnn_

Cuts at:      0     428     738 
   Size:        428     310
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620     738 
   Size:        620     118
 
EcoO109I rG'GnC_Cy

Cuts at:      0     442     738 
   Size:        442     296
 
HaeII r_GCGC'y

Cuts at:      0      20     738 
   Size:         20     718
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279     693     725     738 
   Size:        247      32     414      32      13

  Fragments arranged by size: 

                 414     247      32      32      13
 
HincII GTy'rAC

Cuts at:      0     524     738 
   Size:        524     214
 
MfeI C'AATT_G

Cuts at:      0     620     738 
   Size:        620     118
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     630     738 
   Size:        630     108
 
MspA1I CmG'CkG

Cuts at:      0     253     502     738 
   Size:        253     249     236

  Fragments arranged by size: 

                 253     249     236
 
NspI r_CATG'y

Cuts at:      0     339     602     738 
   Size:        339     263     136

  Fragments arranged by size: 

                 339     263     136
 
PstI C_TGCA'G

Cuts at:      0     471     501     738 
   Size:        471      30     237

  Fragments arranged by size: 

                 471     237      30
 
PvuII CAG'CTG

Cuts at:      0     253     738 
   Size:        253     485
 
SapI GCTCTTCn'nnn_

Cuts at:      0     428     738 
   Size:        428     310
 
SbfI CC_TGCA'GG

Cuts at:      0     471     738 
   Size:        471     267
 
SfcI C'TryA_G

Cuts at:      0     467     497     738 
   Size:        467      30     241

  Fragments arranged by size: 

                 467     241      30
 
StuI AGG'CCT

Cuts at:      0     396     738 
   Size:        396     342
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     123     155     325     357     738 
   Size:        123      32     170      32     381

  Fragments arranged by size: 

                 381     170     123      32      32
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI     AvaI Bme1580I    BsaWI
     BsgI     BsmI Bsp1286I    BspMI    BsrFI    BstYI   Bsu36I     BtgI
     BtsI     DraI     EarI  Eco57MI EcoO109I    HaeII    Hin4I   HincII
     MfeI     MmeI   MspA1I     NspI     PstI    PvuII     SapI     SbfI
     SfcI     StuI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI