Restriction map for
pF1KB6983
(Linear) (Six-base) MAPSORT of: KIBB6983.seq Check: 5708 from: 1 to: 654
KIBB6983.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 607 654
Size: 575 32 47
Fragments arranged by size:
575 47 32
AlwNI CAG_nnn'CTG
Cuts at: 0 378 654
Size: 378 276
ApaI G_GGCC'C
Cuts at: 0 62 654
Size: 62 592
AseI AT'TA_AT
Cuts at: 0 329 654
Size: 329 325
AvaI C'yCGr_G
Cuts at: 0 41 61 654
Size: 41 20 593
Fragments arranged by size:
593 41 20
BanI G'GyrC_C
Cuts at: 0 481 654
Size: 481 173
BanII G_rGCy'C
Cuts at: 0 62 593 604 654
Size: 62 531 11 50
Fragments arranged by size:
531 62 50 11
BbeI G_GCGC'C
Cuts at: 0 485 654
Size: 485 169
BglII A'GATC_T
Cuts at: 0 203 432 654
Size: 203 229 222
Fragments arranged by size:
229 222 203
Bme1580I G_kGCm'C
Cuts at: 0 62 615 654
Size: 62 553 39
Fragments arranged by size:
553 62 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 602 654
Size: 602 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 654
Size: 457 197
BsaAI yAC'GTr
Cuts at: 0 495 654
Size: 495 159
BsaHI Gr'CG_yC
Cuts at: 0 16 482 654
Size: 16 466 172
Fragments arranged by size:
466 172 16
BsaWI w'CCGG_w
Cuts at: 0 569 654
Size: 569 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 575 605 654
Size: 575 30 49
Fragments arranged by size:
575 49 30
BseYI C'CCAG_C
Cuts at: 0 76 225 654
Size: 76 149 429
Fragments arranged by size:
429 149 76
BsiHKAI G_wGCw'C
Cuts at: 0 593 654
Size: 593 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 532 654
Size: 532 122
Bsp1286I G_dGCh'C
Cuts at: 0 62 593 604 615 654
Size: 62 531 11 11 39
Fragments arranged by size:
531 62 39 11 11
BspEI T'CCGG_A
Cuts at: 0 569 654
Size: 569 85
BsrFI r'CCGG_y
Cuts at: 0 335 654
Size: 335 319
BssHII G'CGCG_C
Cuts at: 0 128 654
Size: 128 526
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 378 558 654
Size: 291 87 180 96
Fragments arranged by size:
291 180 96 87
BstYI r'GATC_y
Cuts at: 0 203 432 654
Size: 203 229 222
Fragments arranged by size:
229 222 203
BtgI C'CryG_G
Cuts at: 0 251 303 654
Size: 251 52 351
Fragments arranged by size:
351 251 52
BtsI GCAGTG_nn'
Cuts at: 0 216 654
Size: 216 438
DraIII CAC_nnn'GTG
Cuts at: 0 495 654
Size: 495 159
EaeI y'GGCC_r
Cuts at: 0 300 515 654
Size: 300 215 139
Fragments arranged by size:
300 215 139
EarI CTCTTCn'nnn_
Cuts at: 0 257 370 654
Size: 257 113 284
Fragments arranged by size:
284 257 113
EcoICRI GAG'CTC
Cuts at: 0 591 654
Size: 591 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 654
Size: 457 197
EcoO109I rG'GnC_Cy
Cuts at: 0 52 66 453 654
Size: 52 14 387 201
Fragments arranged by size:
387 201 52 14
HaeII r_GCGC'y
Cuts at: 0 450 485 654
Size: 450 35 169
Fragments arranged by size:
450 169 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 654
Size: 424 32 198
Fragments arranged by size:
424 198 32
KasI G'GCGC_C
Cuts at: 0 481 654
Size: 481 173
MscI TGG'CCA
Cuts at: 0 302 654
Size: 302 352
MspA1I CmG'CkG
Cuts at: 0 8 253 555 654
Size: 8 245 302 99
Fragments arranged by size:
302 245 99 8
NarI GG'CG_CC
Cuts at: 0 482 654
Size: 482 172
NspI r_CATG'y
Cuts at: 0 294 654
Size: 294 360
PflMI CCAn_nnn'nTGG
Cuts at: 0 495 654
Size: 495 159
PfoI T'CCnGG_A
Cuts at: 0 19 242 654
Size: 19 223 412
Fragments arranged by size:
412 223 19
PmlI CAC'GTG
Cuts at: 0 495 654
Size: 495 159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 575 607 654
Size: 575 32 47
Fragments arranged by size:
575 47 32
PpuMI rG'GwC_Cy
Cuts at: 0 52 66 453 654
Size: 52 14 387 201
Fragments arranged by size:
387 201 52 14
PspOMI G'GGCC_C
Cuts at: 0 58 654
Size: 58 596
PstI C_TGCA'G
Cuts at: 0 211 654
Size: 211 443
PvuII CAG'CTG
Cuts at: 0 555 654
Size: 555 99
SacI G_AGCT'C
Cuts at: 0 593 654
Size: 593 61
SacII CC_GC'GG
Cuts at: 0 254 654
Size: 254 400
SanDI GG'GwC_CC
Cuts at: 0 66 654
Size: 66 588
SapI GCTCTTCn'nnn_
Cuts at: 0 370 654
Size: 370 284
SfcI C'TryA_G
Cuts at: 0 207 654
Size: 207 447
SfoI GGC'GCC
Cuts at: 0 483 654
Size: 483 171
SmaI CCC'GGG
Cuts at: 0 43 63 654
Size: 43 20 591
Fragments arranged by size:
591 43 20
StuI AGG'CCT
Cuts at: 0 511 654
Size: 511 143
TatI w'GTAC_w
Cuts at: 0 287 654
Size: 287 367
XmaI C'CCGG_G
Cuts at: 0 41 61 654
Size: 41 20 593
Fragments arranged by size:
593 41 20
Enzymes that do cut:
AloI AlwNI ApaI AseI AvaI BanI BanII BbeI
BglII Bme1580I BmrI BpmI BsaAI BsaHI BsaWI BsaXI
BseYI BsiHKAI BsmBI Bsp1286I BspEI BsrFI BssHII BstAPI
BstYI BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I KasI MscI MspA1I NarI NspI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SapI SfcI SfoI SmaI StuI
TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BseRI BsgI BsiEI BsiWI BsmI
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmnI ZraI