Restriction map for
pF1KB6984
(Linear) (Six-base) MAPSORT of: KIBB6984.seq Check: 3730 from: 1 to: 741
KIBB6984.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 0 683 695 741
Size: 683 12 46
Fragments arranged by size:
683 46 12
AflIII A'CryG_T
Cuts at: 0 545 741
Size: 545 196
AleI CACnn'nnGTG
Cuts at: 0 140 741
Size: 140 601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 204 741
Size: 204 537
AlwNI CAG_nnn'CTG
Cuts at: 0 403 480 741
Size: 403 77 261
Fragments arranged by size:
403 261 77
ApoI r'AATT_y
Cuts at: 0 96 123 741
Size: 96 27 618
Fragments arranged by size:
618 96 27
BanI G'GyrC_C
Cuts at: 0 451 741
Size: 451 290
BanII G_rGCy'C
Cuts at: 0 448 741
Size: 448 293
BbeI G_GCGC'C
Cuts at: 0 455 741
Size: 455 286
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 741
Size: 310 431
BlpI GC'TnA_GC
Cuts at: 0 496 741
Size: 496 245
Bme1580I G_kGCm'C
Cuts at: 0 663 741
Size: 663 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 226 741
Size: 226 515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 380 698 741
Size: 262 118 318 43
Fragments arranged by size:
318 262 118 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 741
Size: 586 155
BsaAI yAC'GTr
Cuts at: 0 346 741
Size: 346 395
BsaHI Gr'CG_yC
Cuts at: 0 452 741
Size: 452 289
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 378 408 741
Size: 378 30 333
Fragments arranged by size:
378 333 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 330 741
Size: 106 224 411
Fragments arranged by size:
411 224 106
BseYI C'CCAG_C
Cuts at: 0 428 741
Size: 428 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 741
Size: 368 373
BsiHKAI G_wGCw'C
Cuts at: 0 227 448 741
Size: 227 221 293
Fragments arranged by size:
293 227 221
Bsp1286I G_dGCh'C
Cuts at: 0 227 448 663 741
Size: 227 221 215 78
Fragments arranged by size:
227 221 215 78
BsrDI GCAATG_nn'
Cuts at: 0 624 741
Size: 624 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 413 741
Size: 413 328
BstXI CCAn_nnnn'nTGG
Cuts at: 0 142 741
Size: 142 599
BtsI GCAGTG_nn'
Cuts at: 0 186 437 481 741
Size: 186 251 44 260
Fragments arranged by size:
260 251 186 44
DraIII CAC_nnn'GTG
Cuts at: 0 413 741
Size: 413 328
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 271 741
Size: 271 470
EcoICRI GAG'CTC
Cuts at: 0 446 741
Size: 446 295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 380 698 741
Size: 262 118 318 43
Fragments arranged by size:
318 262 118 43
EcoRI G'AATT_C
Cuts at: 0 96 123 741
Size: 96 27 618
Fragments arranged by size:
618 96 27
HaeII r_GCGC'y
Cuts at: 0 455 741
Size: 455 286
KasI G'GCGC_C
Cuts at: 0 451 741
Size: 451 290
MslI CAynn'nnrTG
Cuts at: 0 140 208 741
Size: 140 68 533
Fragments arranged by size:
533 140 68
MspA1I CmG'CkG
Cuts at: 0 34 741
Size: 34 707
NarI GG'CG_CC
Cuts at: 0 452 741
Size: 452 289
NspI r_CATG'y
Cuts at: 0 549 741
Size: 549 192
PciI A'CATG_T
Cuts at: 0 545 741
Size: 545 196
PflMI CCAn_nnn'nTGG
Cuts at: 0 403 741
Size: 403 338
PfoI T'CCnGG_A
Cuts at: 0 239 741
Size: 239 502
PmlI CAC'GTG
Cuts at: 0 346 741
Size: 346 395
PstI C_TGCA'G
Cuts at: 0 476 741
Size: 476 265
PvuII CAG'CTG
Cuts at: 0 34 741
Size: 34 707
SacI G_AGCT'C
Cuts at: 0 448 741
Size: 448 293
SexAI A'CCwGG_T
Cuts at: 0 110 741
Size: 110 631
SfcI C'TryA_G
Cuts at: 0 472 741
Size: 472 269
SfoI GGC'GCC
Cuts at: 0 453 741
Size: 453 288
SmlI C'TyrA_G
Cuts at: 0 565 741
Size: 565 176
StyI C'CwwG_G
Cuts at: 0 630 689 741
Size: 630 59 52
Fragments arranged by size:
630 59 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 159 741
Size: 159 582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 378 410 741
Size: 31 347 32 331
Fragments arranged by size:
347 331 32 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 142 741
Size: 142 599
Enzymes that do cut:
AccI AflIII AleI AlfI AlwNI ApoI BanI BanII
BbeI BbsI BlpI Bme1580I BmrI BpmI BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI
BstAPI BstXI BtsI DraIII EciI EcoICRI Eco57MI EcoRI
HaeII KasI MslI MspA1I NarI NspI PciI PflMI
PfoI PmlI PstI PvuII SacI SexAI SfcI SfoI
SmlI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI Bpu10I BsaI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI
EaeI EagI EarI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI