(Linear) (Six-base) MAPSORT of: KIBB6985.seq  Check: 4456  from: 1  to: 657



KIBB6985.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     576     657 
   Size:        576      81
 
AlwNI CAG_nnn'CTG

Cuts at:      0     562     657 
   Size:        562      95
 
ApoI r'AATT_y

Cuts at:      0     628     657 
   Size:        628      29
 
BanI G'GyrC_C

Cuts at:      0     460     657 
   Size:        460     197
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     539     657 
   Size:        539     118
 
BlpI GC'TnA_GC

Cuts at:      0     119     657 
   Size:        119     538
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     340     657 
   Size:        340     317
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     605     635     657 
   Size:        605      30      22

  Fragments arranged by size: 

                 605      30      22
 
BseYI C'CCAG_C

Cuts at:      0      43     657 
   Size:         43     614
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548     657 
   Size:        548     109
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     576     657 
   Size:        576      81
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     562     657 
   Size:        562      95
 
BstYI r'GATC_y

Cuts at:      0     644     657 
   Size:        644      13
 
BtgI C'CryG_G

Cuts at:      0     311     657 
   Size:        311     346
 
DraI TTT'AAA

Cuts at:      0     206     657 
   Size:        206     451
 
DraIII CAC_nnn'GTG

Cuts at:      0     511     657 
   Size:        511     146
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     657 
   Size:         16     641
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      26     657 
   Size:         26     631
 
EcoO109I rG'GnC_Cy

Cuts at:      0     163     657 
   Size:        163     494
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     147     179     544     576     657 
   Size:        147      32     365      32      81

  Fragments arranged by size: 

                 365     147      81      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     657 
   Size:        479     178
 
MspA1I CmG'CkG

Cuts at:      0     234     657 
   Size:        234     423
 
PpuMI rG'GwC_Cy

Cuts at:      0     163     657 
   Size:        163     494
 
StyI C'CwwG_G

Cuts at:      0      29     657 
   Size:         29     628
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     605     637     657 
   Size:        605      32      20

  Fragments arranged by size: 

                 605      32      20
 
XmnI GAAnn'nnTTC

Cuts at:      0     584     657 
   Size:        584      73
 

 Enzymes that do cut:

     AarI    AlwNI     ApoI     BanI     BbsI     BlpI     BmrI    BsaXI
    BseYI     BsgI    BspMI   BstAPI    BstYI     BtgI     DraI   DraIII
     EarI     EciI EcoO109I    Hin4I     MmeI   MspA1I    PpuMI     StyI
     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DrdI     EaeI     EagI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI