Restriction map for
pF1KB6989
(Linear) (Six-base) MAPSORT of: KIBB6989.seq Check: 4544 from: 1 to: 657
KIBB6989.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 159 657
Size: 159 498
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 652 657
Size: 652 5
AlwNI CAG_nnn'CTG
Cuts at: 0 208 657
Size: 208 449
ApaLI G'TGCA_C
Cuts at: 0 188 657
Size: 188 469
ApoI r'AATT_y
Cuts at: 0 643 657
Size: 643 14
AvaI C'yCGr_G
Cuts at: 0 35 113 502 657
Size: 35 78 389 155
Fragments arranged by size:
389 155 78 35
BanII G_rGCy'C
Cuts at: 0 254 657
Size: 254 403
BglI GCCn_nnn'nGGC
Cuts at: 0 232 557 657
Size: 232 325 100
Fragments arranged by size:
325 232 100
Bme1580I G_kGCm'C
Cuts at: 0 192 657
Size: 192 465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 289 657
Size: 268 21 368
Fragments arranged by size:
368 268 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 657
Size: 546 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 374 404 657
Size: 374 30 253
Fragments arranged by size:
374 253 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 657
Size: 520 137
BseYI C'CCAG_C
Cuts at: 0 365 657
Size: 365 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 329 531 657
Size: 154 175 202 126
Fragments arranged by size:
202 175 154 126
BsiHKAI G_wGCw'C
Cuts at: 0 18 192 254 657
Size: 18 174 62 403
Fragments arranged by size:
403 174 62 18
BsmI GAATG_Cn'
Cuts at: 0 416 657
Size: 416 241
Bsp1286I G_dGCh'C
Cuts at: 0 18 192 254 657
Size: 18 174 62 403
Fragments arranged by size:
403 174 62 18
BsrDI GCAATG_nn'
Cuts at: 0 636 657
Size: 636 21
BsrFI r'CCGG_y
Cuts at: 0 601 657
Size: 601 56
BssSI C'ACGA_G
Cuts at: 0 172 657
Size: 172 485
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 657
Size: 69 588
BtgI C'CryG_G
Cuts at: 0 221 255 657
Size: 221 34 402
Fragments arranged by size:
402 221 34
BtsI GCAGTG_nn'
Cuts at: 0 80 657
Size: 80 577
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 269 304 657
Size: 269 35 353
Fragments arranged by size:
353 269 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 616 657
Size: 616 41
EaeI y'GGCC_r
Cuts at: 0 224 233 542 657
Size: 224 9 309 115
Fragments arranged by size:
309 224 115 9
EcoICRI GAG'CTC
Cuts at: 0 252 657
Size: 252 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 289 657
Size: 268 21 368
Fragments arranged by size:
368 268 21
HaeII r_GCGC'y
Cuts at: 0 519 657
Size: 519 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 325 357 657
Size: 145 32 148 32 300
Fragments arranged by size:
300 148 145 32 32
MscI TGG'CCA
Cuts at: 0 544 657
Size: 544 113
MslI CAynn'nnrTG
Cuts at: 0 67 73 657
Size: 67 6 584
Fragments arranged by size:
584 67 6
MspA1I CmG'CkG
Cuts at: 0 168 205 246 598 657
Size: 168 37 41 352 59
Fragments arranged by size:
352 168 59 41 37
NcoI C'CATG_G
Cuts at: 0 255 657
Size: 255 402
NspI r_CATG'y
Cuts at: 0 498 657
Size: 498 159
PasI CC'CwG_GG
Cuts at: 0 402 657
Size: 402 255
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 657
Size: 222 435
PstI C_TGCA'G
Cuts at: 0 126 657
Size: 126 531
PvuII CAG'CTG
Cuts at: 0 205 246 598 657
Size: 205 41 352 59
Fragments arranged by size:
352 205 59 41
SacI G_AGCT'C
Cuts at: 0 254 657
Size: 254 403
SexAI A'CCwGG_T
Cuts at: 0 581 657
Size: 581 76
SfcI C'TryA_G
Cuts at: 0 122 657
Size: 122 535
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 232 657
Size: 232 425
SmaI CCC'GGG
Cuts at: 0 37 657
Size: 37 620
SmlI C'TyrA_G
Cuts at: 0 561 657
Size: 561 96
StyI C'CwwG_G
Cuts at: 0 255 657
Size: 255 402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 72 657
Size: 72 585
XmaI C'CCGG_G
Cuts at: 0 35 657
Size: 35 622
Enzymes that do cut:
AhdI AlfI AlwNI ApaLI ApoI AvaI BanII BglI
Bme1580I BpmI BpuEI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BsrFI BssSI BstXI BtgI BtsI
CspCI DrdI EaeI EcoICRI Eco57MI HaeII Hin4I MscI
MslI MspA1I NcoI NspI PasI PflMI PstI PvuII
SacI SexAI SfcI SfiI SmaI SmlI StyI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
DraI DraIII EagI EarI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmnI ZraI