Restriction map for
pF1KB6992
(Linear) (Six-base) MAPSORT of: KIBB6992.seq Check: 4066 from: 1 to: 756
KIBB6992.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AfeI AGC'GCT
Cuts at: 0 177 482 756
Size: 177 305 274
Fragments arranged by size:
305 274 177
ApaI G_GGCC'C
Cuts at: 0 312 756
Size: 312 444
AvaI C'yCGr_G
Cuts at: 0 380 756
Size: 380 376
BanI G'GyrC_C
Cuts at: 0 267 756
Size: 267 489
BanII G_rGCy'C
Cuts at: 0 312 388 756
Size: 312 76 368
Fragments arranged by size:
368 312 76
BbeI G_GCGC'C
Cuts at: 0 271 756
Size: 271 485
BlpI GC'TnA_GC
Cuts at: 0 60 756
Size: 60 696
Bme1580I G_kGCm'C
Cuts at: 0 312 756
Size: 312 444
BmrI ACTGGGnnnn_n'
Cuts at: 0 293 756
Size: 293 463
Bpu10I CC'TnA_GC
Cuts at: 0 187 756
Size: 187 569
BsaHI Gr'CG_yC
Cuts at: 0 268 756
Size: 268 488
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 545 575 756
Size: 545 30 181
Fragments arranged by size:
545 181 30
BsiEI CG_ry'CG
Cuts at: 0 138 166 756
Size: 138 28 590
Fragments arranged by size:
590 138 28
BsiHKAI G_wGCw'C
Cuts at: 0 365 756
Size: 365 391
BsmBI CGTCTCn'nnnn_
Cuts at: 0 53 756
Size: 53 703
Bsp1286I G_dGCh'C
Cuts at: 0 312 365 388 756
Size: 312 53 23 368
Fragments arranged by size:
368 312 53 23
BsrGI T'GTAC_A
Cuts at: 0 413 756
Size: 413 343
BssHII G'CGCG_C
Cuts at: 0 391 393 756
Size: 391 2 363
Fragments arranged by size:
391 363 2
Bsu36I CC'TnA_GG
Cuts at: 0 248 756
Size: 248 508
BtgI C'CryG_G
Cuts at: 0 104 201 368 398 514 756
Size: 104 97 167 30 116 242
Fragments arranged by size:
242 167 116 104 97 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 79 756
Size: 79 677
EaeI y'GGCC_r
Cuts at: 0 135 163 180 756
Size: 135 28 17 576
Fragments arranged by size:
576 135 28 17
EagI C'GGCC_G
Cuts at: 0 135 163 756
Size: 135 28 593
Fragments arranged by size:
593 135 28
EarI CTCTTCn'nnn_
Cuts at: 0 498 756
Size: 498 258
HaeII r_GCGC'y
Cuts at: 0 179 271 484 756
Size: 179 92 213 272
Fragments arranged by size:
272 213 179 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 545 577 663 695 756
Size: 545 32 86 32 61
Fragments arranged by size:
545 86 61 32 32
KasI G'GCGC_C
Cuts at: 0 267 756
Size: 267 489
MspA1I CmG'CkG
Cuts at: 0 106 168 203 226 400 756
Size: 106 62 35 23 174 356
Fragments arranged by size:
356 174 106 62 35 23
NarI GG'CG_CC
Cuts at: 0 268 756
Size: 268 488
NspI r_CATG'y
Cuts at: 0 336 756
Size: 336 420
PspOMI G'GGCC_C
Cuts at: 0 308 756
Size: 308 448
SacII CC_GC'GG
Cuts at: 0 107 204 401 756
Size: 107 97 197 355
Fragments arranged by size:
355 197 107 97
SfoI GGC'GCC
Cuts at: 0 269 756
Size: 269 487
SmaI CCC'GGG
Cuts at: 0 382 756
Size: 382 374
SphI G_CATG'C
Cuts at: 0 336 756
Size: 336 420
StyI C'CwwG_G
Cuts at: 0 192 704 756
Size: 192 512 52
Fragments arranged by size:
512 192 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 482 756
Size: 312 170 274
Fragments arranged by size:
312 274 170
TatI w'GTAC_w
Cuts at: 0 211 413 597 756
Size: 211 202 184 159
Fragments arranged by size:
211 202 184 159
XmaI C'CCGG_G
Cuts at: 0 380 756
Size: 380 376
XmnI GAAnn'nnTTC
Cuts at: 0 695 756
Size: 695 61
Enzymes that do cut:
AfeI ApaI AvaI BanI BanII BbeI BlpI Bme1580I
BmrI Bpu10I BsaHI BsaXI BsiEI BsiHKAI BsmBI Bsp1286I
BsrGI BssHII Bsu36I BtgI BtgZI EaeI EagI EarI
HaeII Hin4I KasI MspA1I NarI NspI PspOMI SacII
SfoI SmaI SphI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BseRI
BseYI BsgI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI ZraI