(Linear) (Six-base) MAPSORT of: KIBB6997.seq  Check: 2972  from: 1  to: 633



KIBB6997.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     207     305     633 
   Size:        207      98     328

  Fragments arranged by size: 

                 328     207      98
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     384     633 
   Size:        167     217     249

  Fragments arranged by size: 

                 249     217     167
 
AlwNI CAG_nnn'CTG

Cuts at:      0     297     633 
   Size:        297     336
 
BglI GCCn_nnn'nGGC

Cuts at:      0     443     633 
   Size:        443     190
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330     633 
   Size:        330     303
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     109     633 
   Size:         85      24     524

  Fragments arranged by size: 

                 524      85      24
 
BseYI C'CCAG_C

Cuts at:      0     527     633 
   Size:        527     106
 
BsmI GAATG_Cn'

Cuts at:      0     321     633 
   Size:        321     312
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      66     207     305     633 
   Size:         66     141      98     328

  Fragments arranged by size: 

                 328     141      98      66
 
BsrDI GCAATG_nn'

Cuts at:      0     497     558     633 
   Size:        497      61      75

  Fragments arranged by size: 

                 497      75      61
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     297     316     633 
   Size:        297      19     317

  Fragments arranged by size: 

                 317     297      19
 
BstEII G'GTnAC_C

Cuts at:      0     180     459     633 
   Size:        180     279     174

  Fragments arranged by size: 

                 279     180     174
 
BtgI C'CryG_G

Cuts at:      0     209     305     633 
   Size:        209      96     328

  Fragments arranged by size: 

                 328     209      96
 
BtsI GCAGTG_nn'

Cuts at:      0     492     633 
   Size:        492     141
 
EarI CTCTTCn'nnn_

Cuts at:      0     395     633 
   Size:        395     238
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     330     384     633 
   Size:        167     163      54     249

  Fragments arranged by size: 

                 249     167     163      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     454     633 
   Size:        454     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0      22     633 
   Size:         22     611
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     215     247     633 
   Size:        215      32     386

  Fragments arranged by size: 

                 386     215      32
 
MfeI C'AATT_G

Cuts at:      0     369     633 
   Size:        369     264
 
NcoI C'CATG_G

Cuts at:      0     209     633 
   Size:        209     424
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     609     633 
   Size:        609      24
 
PpuMI rG'GwC_Cy

Cuts at:      0      22     633 
   Size:         22     611
 
PstI C_TGCA'G

Cuts at:      0     302     633 
   Size:        302     331
 
SapI GCTCTTCn'nnn_

Cuts at:      0     395     633 
   Size:        395     238
 
ScaI AGT'ACT

Cuts at:      0     328     633 
   Size:        328     305
 
SfcI C'TryA_G

Cuts at:      0     298     633 
   Size:        298     335
 
StyI C'CwwG_G

Cuts at:      0     209     633 
   Size:        209     424
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     519     574     633 
   Size:        519      55      59

  Fragments arranged by size: 

                 519      59      55
 
TatI w'GTAC_w

Cuts at:      0     326     633 
   Size:        326     307
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     216     633 
   Size:        216     417
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     BglI     BpmI    BseRI    BseYI     BsmI
    BspMI    BsrDI   BstAPI   BstEII     BtgI     BtsI     EarI  Eco57MI
    EcoNI EcoO109I    Hin4I     MfeI     NcoI    PflMI    PpuMI     PstI
     SapI     ScaI     SfcI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI