Restriction map for
pF1KB7001
(Linear) (Six-base) MAPSORT of: KIBB7001.seq Check: 1030 from: 1 to: 3921
KIBB7001.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 828 1753 2658 3921
Size: 828 925 905 1263
Fragments arranged by size:
1263 925 905 828
AatII G_ACGT'C
Cuts at: 0 2704 3921
Size: 2704 1217
AccI GT'mk_AC
Cuts at: 0 512 3921
Size: 512 3409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 2522 3476 3550 3846 3921
Size: 640 1882 954 74 296 75
Fragments arranged by size:
1882 954 640 296 75 74
AflIII A'CryG_T
Cuts at: 0 1305 3032 3921
Size: 1305 1727 889
Fragments arranged by size:
1727 1305 889
AleI CACnn'nnGTG
Cuts at: 0 1438 3921
Size: 1438 2483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 778 1150 1456 3921
Size: 778 372 306 2465
Fragments arranged by size:
2465 778 372 306
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3379 3411 3921
Size: 3379 32 510
Fragments arranged by size:
3379 510 32
AlwNI CAG_nnn'CTG
Cuts at: 0 377 2900 3921
Size: 377 2523 1021
Fragments arranged by size:
2523 1021 377
ApaI G_GGCC'C
Cuts at: 0 2978 3921
Size: 2978 943
ApaLI G'TGCA_C
Cuts at: 0 415 3921
Size: 415 3506
ApoI r'AATT_y
Cuts at: 0 932 1605 2431 3846 3921
Size: 932 673 826 1415 75
Fragments arranged by size:
1415 932 826 673 75
AvaI C'yCGr_G
Cuts at: 0 1239 1922 3921
Size: 1239 683 1999
Fragments arranged by size:
1999 1239 683
AvrII C'CTAG_G
Cuts at: 0 2781 3921
Size: 2781 1140
BanI G'GyrC_C
Cuts at: 0 1147 1478 1738 1787 2120 3147 3921
Size: 1147 331 260 49 333 1027 774
Fragments arranged by size:
1147 1027 774 333 331 260 49
BanII G_rGCy'C
Cuts at: 0 447 1169 1240 2978 3921
Size: 447 722 71 1738 943
Fragments arranged by size:
1738 943 722 447 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 374 3491 3673 3921
Size: 374 3117 182 248
Fragments arranged by size:
3117 374 248 182
BbvCI CC'TCA_GC
Cuts at: 0 3081 3921
Size: 3081 840
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 486 520 877 911 3790 3824 3921
Size: 486 34 357 34 2879 34 97
Fragments arranged by size:
2879 486 357 97 34 34 34
BfrBI ATG'CAT
Cuts at: 0 986 2789 3494 3921
Size: 986 1803 705 427
Fragments arranged by size:
1803 986 705 427
BglII A'GATC_T
Cuts at: 0 1049 1883 3921
Size: 1049 834 2038
Fragments arranged by size:
2038 1049 834
BlpI GC'TnA_GC
Cuts at: 0 751 1255 3921
Size: 751 504 2666
Fragments arranged by size:
2666 751 504
Bme1580I G_kGCm'C
Cuts at: 0 419 467 1103 1483 2978 3921
Size: 419 48 636 380 1495 943
Fragments arranged by size:
1495 943 636 419 380 48
BmgBI CAC'GTC
Cuts at: 0 3020 3921
Size: 3020 901
BmrI ACTGGGnnnn_n'
Cuts at: 0 312 476 1683 1852 2139 3146 3921
Size: 312 164 1207 169 287 1007 775
Fragments arranged by size:
1207 1007 775 312 287 169 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 217 682 1104 1282 1409 1837 2014
Size: 198 19 465 422 178 127 428 177
Cuts at: 2014 2873 3921
Size: 859 1048
Fragments arranged by size:
1048 859 465 428 422 198 178 177
127 19
Bpu10I CC'TnA_GC
Cuts at: 0 3081 3921
Size: 3081 840
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2635 3921
Size: 2635 1286
BsaI GGTCTCn'nnnn_
Cuts at: 0 671 2650 3921
Size: 671 1979 1271
Fragments arranged by size:
1979 1271 671
BsaBI GATnn'nnATC
Cuts at: 0 2290 3748 3921
Size: 2290 1458 173
Fragments arranged by size:
2290 1458 173
BsaHI Gr'CG_yC
Cuts at: 0 2701 3921
Size: 2701 1220
BsaWI w'CCGG_w
Cuts at: 0 2476 3384 3921
Size: 2476 908 537
Fragments arranged by size:
2476 908 537
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 215 245 632 662 2322 2352 3921
Size: 215 30 387 30 1660 30 1569
Fragments arranged by size:
1660 1569 387 215 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2722 3921
Size: 2722 1199
BseYI C'CCAG_C
Cuts at: 0 162 204 3921
Size: 162 42 3717
Fragments arranged by size:
3717 162 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1462 1918 3921
Size: 1462 456 2003
Fragments arranged by size:
2003 1462 456
BsiHKAI G_wGCw'C
Cuts at: 0 419 2021 2692 3921
Size: 419 1602 671 1229
Fragments arranged by size:
1602 1229 671 419
BsiWI C'GTAC_G
Cuts at: 0 3811 3921
Size: 3811 110
BsmI GAATG_Cn'
Cuts at: 0 405 2789 3630 3642 3921
Size: 405 2384 841 12 279
Fragments arranged by size:
2384 841 405 279 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 883 1029 2385 2692 3921
Size: 883 146 1356 307 1229
Fragments arranged by size:
1356 1229 883 307 146
Bsp1286I G_dGCh'C
Cuts at: 0 419 447 467 1103 1169 1240 1483 2021
Size: 419 28 20 636 66 71 243 538
Cuts at: 2021 2692 2978 3921
Size: 671 286 943
Fragments arranged by size:
943 671 636 538 419 286 243 71
66 28 20
BspEI T'CCGG_A
Cuts at: 0 2476 3384 3921
Size: 2476 908 537
Fragments arranged by size:
2476 908 537
BspHI T'CATG_A
Cuts at: 0 3773 3921
Size: 3773 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 828 1753 2658 3921
Size: 828 925 905 1263
Fragments arranged by size:
1263 925 905 828
BsrBI CCG'CTC
Cuts at: 0 825 3678 3921
Size: 825 2853 243
Fragments arranged by size:
2853 825 243
BsrDI GCAATG_nn'
Cuts at: 0 1071 3119 3921
Size: 1071 2048 802
Fragments arranged by size:
2048 1071 802
BsrFI r'CCGG_y
Cuts at: 0 1804 3921
Size: 1804 2117
BsrGI T'GTAC_A
Cuts at: 0 569 1781 3921
Size: 569 1212 2140
Fragments arranged by size:
2140 1212 569
BssSI C'ACGA_G
Cuts at: 0 1282 3921
Size: 1282 2639
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2900 3921
Size: 2900 1021
BstBI TT'CG_AA
Cuts at: 0 1188 3702 3921
Size: 1188 2514 219
Fragments arranged by size:
2514 1188 219
BstEII G'GTnAC_C
Cuts at: 0 2356 3921
Size: 2356 1565
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1162 1965 2093 3348 3921
Size: 1162 803 128 1255 573
Fragments arranged by size:
1255 1162 803 573 128
BstYI r'GATC_y
Cuts at: 0 1049 1883 3654 3921
Size: 1049 834 1771 267
Fragments arranged by size:
1771 1049 834 267
BtgI C'CryG_G
Cuts at: 0 13 657 978 1958 3476 3573 3635 3921
Size: 13 644 321 980 1518 97 62 286
Fragments arranged by size:
1518 980 644 321 286 97 62 13
BtsI GCAGTG_nn'
Cuts at: 0 275 1725 2043 2164 2877 3635 3743 3921
Size: 275 1450 318 121 713 758 108 178
Fragments arranged by size:
1450 758 713 318 275 178 121 108
ClaI AT'CG_AT
Cuts at: 0 2558 3921
Size: 2558 1363
DraI TTT'AAA
Cuts at: 0 965 1511 2460 3921
Size: 965 546 949 1461
Fragments arranged by size:
1461 965 949 546
DraIII CAC_nnn'GTG
Cuts at: 0 438 3921
Size: 438 3483
EaeI y'GGCC_r
Cuts at: 0 272 552 2874 3921
Size: 272 280 2322 1047
Fragments arranged by size:
2322 1047 280 272
EarI CTCTTCn'nnn_
Cuts at: 0 2609 2825 3324 3921
Size: 2609 216 499 597
Fragments arranged by size:
2609 597 499 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 217 640 682 1104 1282 1409 1837
Size: 198 19 423 42 422 178 127 428
Cuts at: 1837 2014 2522 2873 3476 3550 3846 3921
Size: 177 508 351 603 74 296 75
Fragments arranged by size:
603 508 428 423 422 351 296 198
178 177 127 75 74 42 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2969 3244 3921
Size: 2969 275 677
Fragments arranged by size:
2969 677 275
EcoO109I rG'GnC_Cy
Cuts at: 0 648 956 1572 1846 2054 2974 2975 3578
Size: 648 308 616 274 208 920 1 603
Cuts at: 3578 3921
Size: 343
Fragments arranged by size:
920 648 616 603 343 308 274 208
1
EcoRI G'AATT_C
Cuts at: 0 932 2431 3921
Size: 932 1499 1490
Fragments arranged by size:
1499 1490 932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 990 2141 2552 3921
Size: 990 1151 411 1369
Fragments arranged by size:
1369 1151 990 411
HaeII r_GCGC'y
Cuts at: 0 1937 3921
Size: 1937 1984
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 494 526 582 598 614 630 632 664
Size: 494 32 56 16 16 16 2 32
Cuts at: 664 668 700 1153 1185 2322 2354 3921
Size: 4 32 453 32 1137 32 1567
Fragments arranged by size:
1567 1137 494 453 56 32 32 32
32 32 16 16 16 4 2
HincII GTy'rAC
Cuts at: 0 1116 3921
Size: 1116 2805
HindIII A'AGCT_T
Cuts at: 0 3219 3921
Size: 3219 702
MfeI C'AATT_G
Cuts at: 0 2208 3921
Size: 2208 1713
MluI A'CGCG_T
Cuts at: 0 1305 3921
Size: 1305 2616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 2684 3921
Size: 165 2519 1237
Fragments arranged by size:
2519 1237 165
MscI TGG'CCA
Cuts at: 0 274 3921
Size: 274 3647
MslI CAynn'nnrTG
Cuts at: 0 1438 2807 3346 3921
Size: 1438 1369 539 575
Fragments arranged by size:
1438 1369 575 539
MspA1I CmG'CkG
Cuts at: 0 1124 1835 1992 2730 3596 3921
Size: 1124 711 157 738 866 325
Fragments arranged by size:
1124 866 738 711 325 157
NaeI GCC'GGC
Cuts at: 0 1806 3921
Size: 1806 2115
NcoI C'CATG_G
Cuts at: 0 657 978 1958 3476 3573 3921
Size: 657 321 980 1518 97 348
Fragments arranged by size:
1518 980 657 348 321 97
NgoMIV G'CCGG_C
Cuts at: 0 1804 3921
Size: 1804 2117
NsiI A_TGCA'T
Cuts at: 0 988 2791 3496 3921
Size: 988 1803 705 425
Fragments arranged by size:
1803 988 705 425
NspI r_CATG'y
Cuts at: 0 636 3036 3498 3921
Size: 636 2400 462 423
Fragments arranged by size:
2400 636 462 423
PasI CC'CwG_GG
Cuts at: 0 777 1599 3921
Size: 777 822 2322
Fragments arranged by size:
2322 822 777
PciI A'CATG_T
Cuts at: 0 3032 3921
Size: 3032 889
PfoI T'CCnGG_A
Cuts at: 0 788 1693 3921
Size: 788 905 2228
Fragments arranged by size:
2228 905 788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2365 2397 3921
Size: 2365 32 1524
Fragments arranged by size:
2365 1524 32
PpuMI rG'GwC_Cy
Cuts at: 0 648 956 1572 1846 2054 3578 3921
Size: 648 308 616 274 208 1524 343
Fragments arranged by size:
1524 648 616 343 308 274 208
PsiI TTA'TAA
Cuts at: 0 3374 3406 3921
Size: 3374 32 515
Fragments arranged by size:
3374 515 32
PspOMI G'GGCC_C
Cuts at: 0 2974 3921
Size: 2974 947
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2073 2105 3921
Size: 2073 32 1816
Fragments arranged by size:
2073 1816 32
PstI C_TGCA'G
Cuts at: 0 1720 2050 2669 2729 2884 3921
Size: 1720 330 619 60 155 1037
Fragments arranged by size:
1720 1037 619 330 155 60
PvuII CAG'CTG
Cuts at: 0 1992 2730 3596 3921
Size: 1992 738 866 325
Fragments arranged by size:
1992 866 738 325
SbfI CC_TGCA'GG
Cuts at: 0 2669 3921
Size: 2669 1252
ScaI AGT'ACT
Cuts at: 0 597 3921
Size: 597 3324
SexAI A'CCwGG_T
Cuts at: 0 2092 3921
Size: 2092 1829
SfcI C'TryA_G
Cuts at: 0 1716 2046 2665 2725 2880 3921
Size: 1716 330 619 60 155 1041
Fragments arranged by size:
1716 1041 619 330 155 60
SmlI C'TyrA_G
Cuts at: 0 2650 3921
Size: 2650 1271
SphI G_CATG'C
Cuts at: 0 636 3921
Size: 636 3285
SspI AAT'ATT
Cuts at: 0 3913 3921
Size: 3913 8
StyI C'CwwG_G
Cuts at: 0 657 689 881 978 1530 1568 1958 1964
Size: 657 32 192 97 552 38 390 6
Cuts at: 1964 2683 2781 3110 3419 3476 3573 3921
Size: 719 98 329 309 57 97 348
Fragments arranged by size:
719 657 552 390 348 329 309 192
98 97 97 57 38 32 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 294 721 801 1428 2375 3165 3921
Size: 294 427 80 627 947 790 756
Fragments arranged by size:
947 790 756 627 427 294 80
TatI w'GTAC_w
Cuts at: 0 356 569 595 1781 1859 3509 3921
Size: 356 213 26 1186 78 1650 412
Fragments arranged by size:
1650 1186 412 356 213 78 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 468 500 857 889 1596 1628 3921
Size: 468 32 357 32 707 32 2293
Fragments arranged by size:
2293 707 468 357 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1433 2093 3921
Size: 1433 660 1828
Fragments arranged by size:
1828 1433 660
XmnI GAAnn'nnTTC
Cuts at: 0 973 2776 3708 3921
Size: 973 1803 932 213
Fragments arranged by size:
1803 973 932 213
ZraI GAC'GTC
Cuts at: 0 2702 3921
Size: 2702 1219
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII
BbsI BbvCI BcgI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtsI
ClaI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PasI PciI PfoI PpiI PpuMI PsiI PspOMI
PsrI PstI PvuII SbfI ScaI SexAI SfcI SmlI
SphI SspI StyI TaqII TatI TstI XcmI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AscI AseI
AsiSI BaeI BamHI BbeI BciVI BclI BglI BmtI
BplI BsaAI BsiEI BssHII BstZ17I Bsu36I BtgZI CspCI
DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI
HpaI KasI KpnI NarI NdeI NheI NotI NruI
PacI PflMI PmeI PmlI PshAI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I
XbaI XhoI XmaI