Restriction map for pF1KB7001
 (Linear) (Six-base) MAPSORT of: KIBB7001.seq  Check: 1030  from: 1  to: 3921



KIBB7001.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     828    1753    2658    3921 
   Size:        828     925     905    1263

  Fragments arranged by size: 

                1263     925     905     828
 
AatII G_ACGT'C

Cuts at:      0    2704    3921 
   Size:       2704    1217
 
AccI GT'mk_AC

Cuts at:      0     512    3921 
   Size:        512    3409
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640    2522    3476    3550    3846    3921 
   Size:        640    1882     954      74     296      75

  Fragments arranged by size: 

                1882     954     640     296      75      74
 
AflIII A'CryG_T

Cuts at:      0    1305    3032    3921 
   Size:       1305    1727     889

  Fragments arranged by size: 

                1727    1305     889
 
AleI CACnn'nnGTG

Cuts at:      0    1438    3921 
   Size:       1438    2483
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     778    1150    1456    3921 
   Size:        778     372     306    2465

  Fragments arranged by size: 

                2465     778     372     306
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3379    3411    3921 
   Size:       3379      32     510

  Fragments arranged by size: 

                3379     510      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     377    2900    3921 
   Size:        377    2523    1021

  Fragments arranged by size: 

                2523    1021     377
 
ApaI G_GGCC'C

Cuts at:      0    2978    3921 
   Size:       2978     943
 
ApaLI G'TGCA_C

Cuts at:      0     415    3921 
   Size:        415    3506
 
ApoI r'AATT_y

Cuts at:      0     932    1605    2431    3846    3921 
   Size:        932     673     826    1415      75

  Fragments arranged by size: 

                1415     932     826     673      75
 
AvaI C'yCGr_G

Cuts at:      0    1239    1922    3921 
   Size:       1239     683    1999

  Fragments arranged by size: 

                1999    1239     683
 
AvrII C'CTAG_G

Cuts at:      0    2781    3921 
   Size:       2781    1140
 
BanI G'GyrC_C

Cuts at:      0    1147    1478    1738    1787    2120    3147    3921 
   Size:       1147     331     260      49     333    1027     774

  Fragments arranged by size: 

                1147    1027     774     333     331     260      49
 
BanII G_rGCy'C

Cuts at:      0     447    1169    1240    2978    3921 
   Size:        447     722      71    1738     943

  Fragments arranged by size: 

                1738     943     722     447      71
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     374    3491    3673    3921 
   Size:        374    3117     182     248

  Fragments arranged by size: 

                3117     374     248     182
 
BbvCI CC'TCA_GC

Cuts at:      0    3081    3921 
   Size:       3081     840
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     486     520     877     911    3790    3824    3921 
   Size:        486      34     357      34    2879      34      97

  Fragments arranged by size: 

                2879     486     357      97      34      34      34
 
BfrBI ATG'CAT

Cuts at:      0     986    2789    3494    3921 
   Size:        986    1803     705     427

  Fragments arranged by size: 

                1803     986     705     427
 
BglII A'GATC_T

Cuts at:      0    1049    1883    3921 
   Size:       1049     834    2038

  Fragments arranged by size: 

                2038    1049     834
 
BlpI GC'TnA_GC

Cuts at:      0     751    1255    3921 
   Size:        751     504    2666

  Fragments arranged by size: 

                2666     751     504
 
Bme1580I G_kGCm'C

Cuts at:      0     419     467    1103    1483    2978    3921 
   Size:        419      48     636     380    1495     943

  Fragments arranged by size: 

                1495     943     636     419     380      48
 
BmgBI CAC'GTC

Cuts at:      0    3020    3921 
   Size:       3020     901
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     312     476    1683    1852    2139    3146    3921 
   Size:        312     164    1207     169     287    1007     775

  Fragments arranged by size: 

                1207    1007     775     312     287     169     164
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     217     682    1104    1282    1409    1837    2014 
   Size:        198      19     465     422     178     127     428     177

Cuts at:   2014    2873    3921 
   Size:        859    1048

  Fragments arranged by size: 

                1048     859     465     428     422     198     178     177
                 127      19
 
Bpu10I CC'TnA_GC

Cuts at:      0    3081    3921 
   Size:       3081     840
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2635    3921 
   Size:       2635    1286
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     671    2650    3921 
   Size:        671    1979    1271

  Fragments arranged by size: 

                1979    1271     671
 
BsaBI GATnn'nnATC

Cuts at:      0    2290    3748    3921 
   Size:       2290    1458     173

  Fragments arranged by size: 

                2290    1458     173
 
BsaHI Gr'CG_yC

Cuts at:      0    2701    3921 
   Size:       2701    1220
 
BsaWI w'CCGG_w

Cuts at:      0    2476    3384    3921 
   Size:       2476     908     537

  Fragments arranged by size: 

                2476     908     537
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     215     245     632     662    2322    2352    3921 
   Size:        215      30     387      30    1660      30    1569

  Fragments arranged by size: 

                1660    1569     387     215      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2722    3921 
   Size:       2722    1199
 
BseYI C'CCAG_C

Cuts at:      0     162     204    3921 
   Size:        162      42    3717

  Fragments arranged by size: 

                3717     162      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1462    1918    3921 
   Size:       1462     456    2003

  Fragments arranged by size: 

                2003    1462     456
 
BsiHKAI G_wGCw'C

Cuts at:      0     419    2021    2692    3921 
   Size:        419    1602     671    1229

  Fragments arranged by size: 

                1602    1229     671     419
 
BsiWI C'GTAC_G

Cuts at:      0    3811    3921 
   Size:       3811     110
 
BsmI GAATG_Cn'

Cuts at:      0     405    2789    3630    3642    3921 
   Size:        405    2384     841      12     279

  Fragments arranged by size: 

                2384     841     405     279      12
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     883    1029    2385    2692    3921 
   Size:        883     146    1356     307    1229

  Fragments arranged by size: 

                1356    1229     883     307     146
 
Bsp1286I G_dGCh'C

Cuts at:      0     419     447     467    1103    1169    1240    1483    2021 
   Size:        419      28      20     636      66      71     243     538

Cuts at:   2021    2692    2978    3921 
   Size:        671     286     943

  Fragments arranged by size: 

                 943     671     636     538     419     286     243      71
                  66      28      20
 
BspEI T'CCGG_A

Cuts at:      0    2476    3384    3921 
   Size:       2476     908     537

  Fragments arranged by size: 

                2476     908     537
 
BspHI T'CATG_A

Cuts at:      0    3773    3921 
   Size:       3773     148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     828    1753    2658    3921 
   Size:        828     925     905    1263

  Fragments arranged by size: 

                1263     925     905     828
 
BsrBI CCG'CTC

Cuts at:      0     825    3678    3921 
   Size:        825    2853     243

  Fragments arranged by size: 

                2853     825     243
 
BsrDI GCAATG_nn'

Cuts at:      0    1071    3119    3921 
   Size:       1071    2048     802

  Fragments arranged by size: 

                2048    1071     802
 
BsrFI r'CCGG_y

Cuts at:      0    1804    3921 
   Size:       1804    2117
 
BsrGI T'GTAC_A

Cuts at:      0     569    1781    3921 
   Size:        569    1212    2140

  Fragments arranged by size: 

                2140    1212     569
 
BssSI C'ACGA_G

Cuts at:      0    1282    3921 
   Size:       1282    2639
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2900    3921 
   Size:       2900    1021
 
BstBI TT'CG_AA

Cuts at:      0    1188    3702    3921 
   Size:       1188    2514     219

  Fragments arranged by size: 

                2514    1188     219
 
BstEII G'GTnAC_C

Cuts at:      0    2356    3921 
   Size:       2356    1565
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1162    1965    2093    3348    3921 
   Size:       1162     803     128    1255     573

  Fragments arranged by size: 

                1255    1162     803     573     128
 
BstYI r'GATC_y

Cuts at:      0    1049    1883    3654    3921 
   Size:       1049     834    1771     267

  Fragments arranged by size: 

                1771    1049     834     267
 
BtgI C'CryG_G

Cuts at:      0      13     657     978    1958    3476    3573    3635    3921 
   Size:         13     644     321     980    1518      97      62     286

  Fragments arranged by size: 

                1518     980     644     321     286      97      62      13
            
 
BtsI GCAGTG_nn'

Cuts at:      0     275    1725    2043    2164    2877    3635    3743    3921 
   Size:        275    1450     318     121     713     758     108     178

  Fragments arranged by size: 

                1450     758     713     318     275     178     121     108
            
 
ClaI AT'CG_AT

Cuts at:      0    2558    3921 
   Size:       2558    1363
 
DraI TTT'AAA

Cuts at:      0     965    1511    2460    3921 
   Size:        965     546     949    1461

  Fragments arranged by size: 

                1461     965     949     546
 
DraIII CAC_nnn'GTG

Cuts at:      0     438    3921 
   Size:        438    3483
 
EaeI y'GGCC_r

Cuts at:      0     272     552    2874    3921 
   Size:        272     280    2322    1047

  Fragments arranged by size: 

                2322    1047     280     272
 
EarI CTCTTCn'nnn_

Cuts at:      0    2609    2825    3324    3921 
   Size:       2609     216     499     597

  Fragments arranged by size: 

                2609     597     499     216
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     217     640     682    1104    1282    1409    1837 
   Size:        198      19     423      42     422     178     127     428

Cuts at:   1837    2014    2522    2873    3476    3550    3846    3921 
   Size:        177     508     351     603      74     296      75

  Fragments arranged by size: 

                 603     508     428     423     422     351     296     198
                 178     177     127      75      74      42      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2969    3244    3921 
   Size:       2969     275     677

  Fragments arranged by size: 

                2969     677     275
 
EcoO109I rG'GnC_Cy

Cuts at:      0     648     956    1572    1846    2054    2974    2975    3578 
   Size:        648     308     616     274     208     920       1     603

Cuts at:   3578    3921 
   Size:        343

  Fragments arranged by size: 

                 920     648     616     603     343     308     274     208
                   1
 
EcoRI G'AATT_C

Cuts at:      0     932    2431    3921 
   Size:        932    1499    1490

  Fragments arranged by size: 

                1499    1490     932
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     990    2141    2552    3921 
   Size:        990    1151     411    1369

  Fragments arranged by size: 

                1369    1151     990     411
 
HaeII r_GCGC'y

Cuts at:      0    1937    3921 
   Size:       1937    1984
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     494     526     582     598     614     630     632     664 
   Size:        494      32      56      16      16      16       2      32

Cuts at:    664     668     700    1153    1185    2322    2354    3921 
   Size:          4      32     453      32    1137      32    1567

  Fragments arranged by size: 

                1567    1137     494     453      56      32      32      32
                  32      32      16      16      16       4       2
 
HincII GTy'rAC

Cuts at:      0    1116    3921 
   Size:       1116    2805
 
HindIII A'AGCT_T

Cuts at:      0    3219    3921 
   Size:       3219     702
 
MfeI C'AATT_G

Cuts at:      0    2208    3921 
   Size:       2208    1713
 
MluI A'CGCG_T

Cuts at:      0    1305    3921 
   Size:       1305    2616
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     165    2684    3921 
   Size:        165    2519    1237

  Fragments arranged by size: 

                2519    1237     165
 
MscI TGG'CCA

Cuts at:      0     274    3921 
   Size:        274    3647
 
MslI CAynn'nnrTG

Cuts at:      0    1438    2807    3346    3921 
   Size:       1438    1369     539     575

  Fragments arranged by size: 

                1438    1369     575     539
 
MspA1I CmG'CkG

Cuts at:      0    1124    1835    1992    2730    3596    3921 
   Size:       1124     711     157     738     866     325

  Fragments arranged by size: 

                1124     866     738     711     325     157
 
NaeI GCC'GGC

Cuts at:      0    1806    3921 
   Size:       1806    2115
 
NcoI C'CATG_G

Cuts at:      0     657     978    1958    3476    3573    3921 
   Size:        657     321     980    1518      97     348

  Fragments arranged by size: 

                1518     980     657     348     321      97
 
NgoMIV G'CCGG_C

Cuts at:      0    1804    3921 
   Size:       1804    2117
 
NsiI A_TGCA'T

Cuts at:      0     988    2791    3496    3921 
   Size:        988    1803     705     425

  Fragments arranged by size: 

                1803     988     705     425
 
NspI r_CATG'y

Cuts at:      0     636    3036    3498    3921 
   Size:        636    2400     462     423

  Fragments arranged by size: 

                2400     636     462     423
 
PasI CC'CwG_GG

Cuts at:      0     777    1599    3921 
   Size:        777     822    2322

  Fragments arranged by size: 

                2322     822     777
 
PciI A'CATG_T

Cuts at:      0    3032    3921 
   Size:       3032     889
 
PfoI T'CCnGG_A

Cuts at:      0     788    1693    3921 
   Size:        788     905    2228

  Fragments arranged by size: 

                2228     905     788
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2365    2397    3921 
   Size:       2365      32    1524

  Fragments arranged by size: 

                2365    1524      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     648     956    1572    1846    2054    3578    3921 
   Size:        648     308     616     274     208    1524     343

  Fragments arranged by size: 

                1524     648     616     343     308     274     208
 
PsiI TTA'TAA

Cuts at:      0    3374    3406    3921 
   Size:       3374      32     515

  Fragments arranged by size: 

                3374     515      32
 
PspOMI G'GGCC_C

Cuts at:      0    2974    3921 
   Size:       2974     947
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2073    2105    3921 
   Size:       2073      32    1816

  Fragments arranged by size: 

                2073    1816      32
 
PstI C_TGCA'G

Cuts at:      0    1720    2050    2669    2729    2884    3921 
   Size:       1720     330     619      60     155    1037

  Fragments arranged by size: 

                1720    1037     619     330     155      60
 
PvuII CAG'CTG

Cuts at:      0    1992    2730    3596    3921 
   Size:       1992     738     866     325

  Fragments arranged by size: 

                1992     866     738     325
 
SbfI CC_TGCA'GG

Cuts at:      0    2669    3921 
   Size:       2669    1252
 
ScaI AGT'ACT

Cuts at:      0     597    3921 
   Size:        597    3324
 
SexAI A'CCwGG_T

Cuts at:      0    2092    3921 
   Size:       2092    1829
 
SfcI C'TryA_G

Cuts at:      0    1716    2046    2665    2725    2880    3921 
   Size:       1716     330     619      60     155    1041

  Fragments arranged by size: 

                1716    1041     619     330     155      60
 
SmlI C'TyrA_G

Cuts at:      0    2650    3921 
   Size:       2650    1271
 
SphI G_CATG'C

Cuts at:      0     636    3921 
   Size:        636    3285
 
SspI AAT'ATT

Cuts at:      0    3913    3921 
   Size:       3913       8
 
StyI C'CwwG_G

Cuts at:      0     657     689     881     978    1530    1568    1958    1964 
   Size:        657      32     192      97     552      38     390       6

Cuts at:   1964    2683    2781    3110    3419    3476    3573    3921 
   Size:        719      98     329     309      57      97     348

  Fragments arranged by size: 

                 719     657     552     390     348     329     309     192
                  98      97      97      57      38      32       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     294     721     801    1428    2375    3165    3921 
   Size:        294     427      80     627     947     790     756

  Fragments arranged by size: 

                 947     790     756     627     427     294      80
 
TatI w'GTAC_w

Cuts at:      0     356     569     595    1781    1859    3509    3921 
   Size:        356     213      26    1186      78    1650     412

  Fragments arranged by size: 

                1650    1186     412     356     213      78      26
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     468     500     857     889    1596    1628    3921 
   Size:        468      32     357      32     707      32    2293

  Fragments arranged by size: 

                2293     707     468     357      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1433    2093    3921 
   Size:       1433     660    1828

  Fragments arranged by size: 

                1828    1433     660
 
XmnI GAAnn'nnTTC

Cuts at:      0     973    2776    3708    3921 
   Size:        973    1803     932     213

  Fragments arranged by size: 

                1803     973     932     213
 
ZraI GAC'GTC

Cuts at:      0    2702    3921 
   Size:       2702    1219
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII     BanI    BanII
     BbsI    BbvCI     BcgI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI     BtsI
     ClaI     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI
     NspI     PasI     PciI     PfoI     PpiI    PpuMI     PsiI   PspOMI
     PsrI     PstI    PvuII     SbfI     ScaI    SexAI     SfcI     SmlI
     SphI     SspI     StyI    TaqII     TatI     TstI     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AscI     AseI
    AsiSI     BaeI    BamHI     BbeI    BciVI     BclI     BglI     BmtI
     BplI    BsaAI    BsiEI   BssHII  BstZ17I   Bsu36I    BtgZI    CspCI
     DrdI     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI
     HpaI     KasI     KpnI     NarI     NdeI     NheI     NotI     NruI
     PacI    PflMI     PmeI     PmlI    PshAI    PspXI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmaI
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