(Linear) (Six-base) MAPSORT of: KIBB7001.seq Check: 1030 from: 1 to: 3921 KIBB7001.orf With 182 enzymes: SgfI * February 28, 2007 11:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 828 1753 2658 3921 Size: 828 925 905 1263 Fragments arranged by size: 1263 925 905 828 AatII G_ACGT'C Cuts at: 0 2704 3921 Size: 2704 1217 AccI GT'mk_AC Cuts at: 0 512 3921 Size: 512 3409 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 2522 3476 3550 3846 3921 Size: 640 1882 954 74 296 75 Fragments arranged by size: 1882 954 640 296 75 74 AflIII A'CryG_T Cuts at: 0 1305 3032 3921 Size: 1305 1727 889 Fragments arranged by size: 1727 1305 889 AleI CACnn'nnGTG Cuts at: 0 1438 3921 Size: 1438 2483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 778 1150 1456 3921 Size: 778 372 306 2465 Fragments arranged by size: 2465 778 372 306 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3379 3411 3921 Size: 3379 32 510 Fragments arranged by size: 3379 510 32 AlwNI CAG_nnn'CTG Cuts at: 0 377 2900 3921 Size: 377 2523 1021 Fragments arranged by size: 2523 1021 377 ApaI G_GGCC'C Cuts at: 0 2978 3921 Size: 2978 943 ApaLI G'TGCA_C Cuts at: 0 415 3921 Size: 415 3506 ApoI r'AATT_y Cuts at: 0 932 1605 2431 3846 3921 Size: 932 673 826 1415 75 Fragments arranged by size: 1415 932 826 673 75 AvaI C'yCGr_G Cuts at: 0 1239 1922 3921 Size: 1239 683 1999 Fragments arranged by size: 1999 1239 683 AvrII C'CTAG_G Cuts at: 0 2781 3921 Size: 2781 1140 BanI G'GyrC_C Cuts at: 0 1147 1478 1738 1787 2120 3147 3921 Size: 1147 331 260 49 333 1027 774 Fragments arranged by size: 1147 1027 774 333 331 260 49 BanII G_rGCy'C Cuts at: 0 447 1169 1240 2978 3921 Size: 447 722 71 1738 943 Fragments arranged by size: 1738 943 722 447 71 BbsI GAAGACnn'nnnn_ Cuts at: 0 374 3491 3673 3921 Size: 374 3117 182 248 Fragments arranged by size: 3117 374 248 182 BbvCI CC'TCA_GC Cuts at: 0 3081 3921 Size: 3081 840 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 486 520 877 911 3790 3824 3921 Size: 486 34 357 34 2879 34 97 Fragments arranged by size: 2879 486 357 97 34 34 34 BfrBI ATG'CAT Cuts at: 0 986 2789 3494 3921 Size: 986 1803 705 427 Fragments arranged by size: 1803 986 705 427 BglII A'GATC_T Cuts at: 0 1049 1883 3921 Size: 1049 834 2038 Fragments arranged by size: 2038 1049 834 BlpI GC'TnA_GC Cuts at: 0 751 1255 3921 Size: 751 504 2666 Fragments arranged by size: 2666 751 504 Bme1580I G_kGCm'C Cuts at: 0 419 467 1103 1483 2978 3921 Size: 419 48 636 380 1495 943 Fragments arranged by size: 1495 943 636 419 380 48 BmgBI CAC'GTC Cuts at: 0 3020 3921 Size: 3020 901 BmrI ACTGGGnnnn_n' Cuts at: 0 312 476 1683 1852 2139 3146 3921 Size: 312 164 1207 169 287 1007 775 Fragments arranged by size: 1207 1007 775 312 287 169 164 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 198 217 682 1104 1282 1409 1837 2014 Size: 198 19 465 422 178 127 428 177 Cuts at: 2014 2873 3921 Size: 859 1048 Fragments arranged by size: 1048 859 465 428 422 198 178 177 127 19 Bpu10I CC'TnA_GC Cuts at: 0 3081 3921 Size: 3081 840 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2635 3921 Size: 2635 1286 BsaI GGTCTCn'nnnn_ Cuts at: 0 671 2650 3921 Size: 671 1979 1271 Fragments arranged by size: 1979 1271 671 BsaBI GATnn'nnATC Cuts at: 0 2290 3748 3921 Size: 2290 1458 173 Fragments arranged by size: 2290 1458 173 BsaHI Gr'CG_yC Cuts at: 0 2701 3921 Size: 2701 1220 BsaWI w'CCGG_w Cuts at: 0 2476 3384 3921 Size: 2476 908 537 Fragments arranged by size: 2476 908 537 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 215 245 632 662 2322 2352 3921 Size: 215 30 387 30 1660 30 1569 Fragments arranged by size: 1660 1569 387 215 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2722 3921 Size: 2722 1199 BseYI C'CCAG_C Cuts at: 0 162 204 3921 Size: 162 42 3717 Fragments arranged by size: 3717 162 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1462 1918 3921 Size: 1462 456 2003 Fragments arranged by size: 2003 1462 456 BsiHKAI G_wGCw'C Cuts at: 0 419 2021 2692 3921 Size: 419 1602 671 1229 Fragments arranged by size: 1602 1229 671 419 BsiWI C'GTAC_G Cuts at: 0 3811 3921 Size: 3811 110 BsmI GAATG_Cn' Cuts at: 0 405 2789 3630 3642 3921 Size: 405 2384 841 12 279 Fragments arranged by size: 2384 841 405 279 12 BsmBI CGTCTCn'nnnn_ Cuts at: 0 883 1029 2385 2692 3921 Size: 883 146 1356 307 1229 Fragments arranged by size: 1356 1229 883 307 146 Bsp1286I G_dGCh'C Cuts at: 0 419 447 467 1103 1169 1240 1483 2021 Size: 419 28 20 636 66 71 243 538 Cuts at: 2021 2692 2978 3921 Size: 671 286 943 Fragments arranged by size: 943 671 636 538 419 286 243 71 66 28 20 BspEI T'CCGG_A Cuts at: 0 2476 3384 3921 Size: 2476 908 537 Fragments arranged by size: 2476 908 537 BspHI T'CATG_A Cuts at: 0 3773 3921 Size: 3773 148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 828 1753 2658 3921 Size: 828 925 905 1263 Fragments arranged by size: 1263 925 905 828 BsrBI CCG'CTC Cuts at: 0 825 3678 3921 Size: 825 2853 243 Fragments arranged by size: 2853 825 243 BsrDI GCAATG_nn' Cuts at: 0 1071 3119 3921 Size: 1071 2048 802 Fragments arranged by size: 2048 1071 802 BsrFI r'CCGG_y Cuts at: 0 1804 3921 Size: 1804 2117 BsrGI T'GTAC_A Cuts at: 0 569 1781 3921 Size: 569 1212 2140 Fragments arranged by size: 2140 1212 569 BssSI C'ACGA_G Cuts at: 0 1282 3921 Size: 1282 2639 BstAPI GCAn_nnn'nTGC Cuts at: 0 2900 3921 Size: 2900 1021 BstBI TT'CG_AA Cuts at: 0 1188 3702 3921 Size: 1188 2514 219 Fragments arranged by size: 2514 1188 219 BstEII G'GTnAC_C Cuts at: 0 2356 3921 Size: 2356 1565 BstXI CCAn_nnnn'nTGG Cuts at: 0 1162 1965 2093 3348 3921 Size: 1162 803 128 1255 573 Fragments arranged by size: 1255 1162 803 573 128 BstYI r'GATC_y Cuts at: 0 1049 1883 3654 3921 Size: 1049 834 1771 267 Fragments arranged by size: 1771 1049 834 267 BtgI C'CryG_G Cuts at: 0 13 657 978 1958 3476 3573 3635 3921 Size: 13 644 321 980 1518 97 62 286 Fragments arranged by size: 1518 980 644 321 286 97 62 13 BtsI GCAGTG_nn' Cuts at: 0 275 1725 2043 2164 2877 3635 3743 3921 Size: 275 1450 318 121 713 758 108 178 Fragments arranged by size: 1450 758 713 318 275 178 121 108 ClaI AT'CG_AT Cuts at: 0 2558 3921 Size: 2558 1363 DraI TTT'AAA Cuts at: 0 965 1511 2460 3921 Size: 965 546 949 1461 Fragments arranged by size: 1461 965 949 546 DraIII CAC_nnn'GTG Cuts at: 0 438 3921 Size: 438 3483 EaeI y'GGCC_r Cuts at: 0 272 552 2874 3921 Size: 272 280 2322 1047 Fragments arranged by size: 2322 1047 280 272 EarI CTCTTCn'nnn_ Cuts at: 0 2609 2825 3324 3921 Size: 2609 216 499 597 Fragments arranged by size: 2609 597 499 216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 198 217 640 682 1104 1282 1409 1837 Size: 198 19 423 42 422 178 127 428 Cuts at: 1837 2014 2522 2873 3476 3550 3846 3921 Size: 177 508 351 603 74 296 75 Fragments arranged by size: 603 508 428 423 422 351 296 198 178 177 127 75 74 42 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 2969 3244 3921 Size: 2969 275 677 Fragments arranged by size: 2969 677 275 EcoO109I rG'GnC_Cy Cuts at: 0 648 956 1572 1846 2054 2974 2975 3578 Size: 648 308 616 274 208 920 1 603 Cuts at: 3578 3921 Size: 343 Fragments arranged by size: 920 648 616 603 343 308 274 208 1 EcoRI G'AATT_C Cuts at: 0 932 2431 3921 Size: 932 1499 1490 Fragments arranged by size: 1499 1490 932 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 990 2141 2552 3921 Size: 990 1151 411 1369 Fragments arranged by size: 1369 1151 990 411 HaeII r_GCGC'y Cuts at: 0 1937 3921 Size: 1937 1984 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 494 526 582 598 614 630 632 664 Size: 494 32 56 16 16 16 2 32 Cuts at: 664 668 700 1153 1185 2322 2354 3921 Size: 4 32 453 32 1137 32 1567 Fragments arranged by size: 1567 1137 494 453 56 32 32 32 32 32 16 16 16 4 2 HincII GTy'rAC Cuts at: 0 1116 3921 Size: 1116 2805 HindIII A'AGCT_T Cuts at: 0 3219 3921 Size: 3219 702 MfeI C'AATT_G Cuts at: 0 2208 3921 Size: 2208 1713 MluI A'CGCG_T Cuts at: 0 1305 3921 Size: 1305 2616 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 165 2684 3921 Size: 165 2519 1237 Fragments arranged by size: 2519 1237 165 MscI TGG'CCA Cuts at: 0 274 3921 Size: 274 3647 MslI CAynn'nnrTG Cuts at: 0 1438 2807 3346 3921 Size: 1438 1369 539 575 Fragments arranged by size: 1438 1369 575 539 MspA1I CmG'CkG Cuts at: 0 1124 1835 1992 2730 3596 3921 Size: 1124 711 157 738 866 325 Fragments arranged by size: 1124 866 738 711 325 157 NaeI GCC'GGC Cuts at: 0 1806 3921 Size: 1806 2115 NcoI C'CATG_G Cuts at: 0 657 978 1958 3476 3573 3921 Size: 657 321 980 1518 97 348 Fragments arranged by size: 1518 980 657 348 321 97 NgoMIV G'CCGG_C Cuts at: 0 1804 3921 Size: 1804 2117 NsiI A_TGCA'T Cuts at: 0 988 2791 3496 3921 Size: 988 1803 705 425 Fragments arranged by size: 1803 988 705 425 NspI r_CATG'y Cuts at: 0 636 3036 3498 3921 Size: 636 2400 462 423 Fragments arranged by size: 2400 636 462 423 PasI CC'CwG_GG Cuts at: 0 777 1599 3921 Size: 777 822 2322 Fragments arranged by size: 2322 822 777 PciI A'CATG_T Cuts at: 0 3032 3921 Size: 3032 889 PfoI T'CCnGG_A Cuts at: 0 788 1693 3921 Size: 788 905 2228 Fragments arranged by size: 2228 905 788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2365 2397 3921 Size: 2365 32 1524 Fragments arranged by size: 2365 1524 32 PpuMI rG'GwC_Cy Cuts at: 0 648 956 1572 1846 2054 3578 3921 Size: 648 308 616 274 208 1524 343 Fragments arranged by size: 1524 648 616 343 308 274 208 PsiI TTA'TAA Cuts at: 0 3374 3406 3921 Size: 3374 32 515 Fragments arranged by size: 3374 515 32 PspOMI G'GGCC_C Cuts at: 0 2974 3921 Size: 2974 947 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2073 2105 3921 Size: 2073 32 1816 Fragments arranged by size: 2073 1816 32 PstI C_TGCA'G Cuts at: 0 1720 2050 2669 2729 2884 3921 Size: 1720 330 619 60 155 1037 Fragments arranged by size: 1720 1037 619 330 155 60 PvuII CAG'CTG Cuts at: 0 1992 2730 3596 3921 Size: 1992 738 866 325 Fragments arranged by size: 1992 866 738 325 SbfI CC_TGCA'GG Cuts at: 0 2669 3921 Size: 2669 1252 ScaI AGT'ACT Cuts at: 0 597 3921 Size: 597 3324 SexAI A'CCwGG_T Cuts at: 0 2092 3921 Size: 2092 1829 SfcI C'TryA_G Cuts at: 0 1716 2046 2665 2725 2880 3921 Size: 1716 330 619 60 155 1041 Fragments arranged by size: 1716 1041 619 330 155 60 SmlI C'TyrA_G Cuts at: 0 2650 3921 Size: 2650 1271 SphI G_CATG'C Cuts at: 0 636 3921 Size: 636 3285 SspI AAT'ATT Cuts at: 0 3913 3921 Size: 3913 8 StyI C'CwwG_G Cuts at: 0 657 689 881 978 1530 1568 1958 1964 Size: 657 32 192 97 552 38 390 6 Cuts at: 1964 2683 2781 3110 3419 3476 3573 3921 Size: 719 98 329 309 57 97 348 Fragments arranged by size: 719 657 552 390 348 329 309 192 98 97 97 57 38 32 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 294 721 801 1428 2375 3165 3921 Size: 294 427 80 627 947 790 756 Fragments arranged by size: 947 790 756 627 427 294 80 TatI w'GTAC_w Cuts at: 0 356 569 595 1781 1859 3509 3921 Size: 356 213 26 1186 78 1650 412 Fragments arranged by size: 1650 1186 412 356 213 78 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 468 500 857 889 1596 1628 3921 Size: 468 32 357 32 707 32 2293 Fragments arranged by size: 2293 707 468 357 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1433 2093 3921 Size: 1433 660 1828 Fragments arranged by size: 1828 1433 660 XmnI GAAnn'nnTTC Cuts at: 0 973 2776 3708 3921 Size: 973 1803 932 213 Fragments arranged by size: 1803 973 932 213 ZraI GAC'GTC Cuts at: 0 2702 3921 Size: 2702 1219 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbsI BbvCI BcgI BfrBI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtsI ClaI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PciI PfoI PpiI PpuMI PsiI PspOMI PsrI PstI PvuII SbfI ScaI SexAI SfcI SmlI SphI SspI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AscI AseI AsiSI BaeI BamHI BbeI BciVI BclI BglI BmtI BplI BsaAI BsiEI BssHII BstZ17I Bsu36I BtgZI CspCI DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI HpaI KasI KpnI NarI NdeI NheI NotI NruI PacI PflMI PmeI PmlI PshAI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XhoI XmaI