(Linear) (Six-base) MAPSORT of: KIBB7002.seq Check: 2178 from: 1 to: 9081 KIBB7002.orf With 182 enzymes: SgfI * December 28, 2009 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 8628 9081 Size: 8628 453 AatII G_ACGT'C Cuts at: 0 5478 9081 Size: 5478 3603 AccI GT'mk_AC Cuts at: 0 188 4708 8030 9081 Size: 188 4520 3322 1051 Fragments arranged by size: 4520 3322 1051 188 Acc65I G'GTAC_C Cuts at: 0 1383 9081 Size: 1383 7698 AclI AA'CG_TT Cuts at: 0 7173 9081 Size: 7173 1908 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 3085 3890 5789 6193 6260 6320 8837 Size: 452 2633 805 1899 404 67 60 2517 Cuts at: 8837 9081 Size: 244 Fragments arranged by size: 2633 2517 1899 805 452 404 244 67 60 AfeI AGC'GCT Cuts at: 0 79 9081 Size: 79 9002 AflIII A'CryG_T Cuts at: 0 2648 3368 4763 7092 9081 Size: 2648 720 1395 2329 1989 Fragments arranged by size: 2648 2329 1989 1395 720 AleI CACnn'nnGTG Cuts at: 0 6973 9081 Size: 6973 2108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 751 1070 8664 9081 Size: 751 319 7594 417 Fragments arranged by size: 7594 751 417 319 AlwNI CAG_nnn'CTG Cuts at: 0 18 1833 5781 6238 7292 7693 8470 8839 Size: 18 1815 3948 457 1054 401 777 369 Cuts at: 8839 8968 9081 Size: 129 113 Fragments arranged by size: 3948 1815 1054 777 457 401 369 129 113 18 ApaLI G'TGCA_C Cuts at: 0 3152 7088 9081 Size: 3152 3936 1993 Fragments arranged by size: 3936 3152 1993 ApoI r'AATT_y Cuts at: 0 279 441 523 541 1586 2369 2655 3586 Size: 279 162 82 18 1045 783 286 931 Cuts at: 3586 4624 5200 6085 6277 6342 6912 7446 7808 Size: 1038 576 885 192 65 570 534 362 Cuts at: 7808 8012 9070 9081 Size: 204 1058 11 Fragments arranged by size: 1058 1045 1038 931 885 783 576 570 534 362 286 279 204 192 162 82 65 18 11 AseI AT'TA_AT Cuts at: 0 869 2183 5368 5549 8231 9081 Size: 869 1314 3185 181 2682 850 Fragments arranged by size: 3185 2682 1314 869 850 181 AvaI C'yCGr_G Cuts at: 0 692 9081 Size: 692 8389 AvrII C'CTAG_G Cuts at: 0 739 9081 Size: 739 8342 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2770 2803 3955 3988 8597 8630 9081 Size: 2770 33 1152 33 4609 33 451 Fragments arranged by size: 4609 2770 1152 451 33 33 33 BamHI G'GATC_C Cuts at: 0 354 1014 2088 6421 9081 Size: 354 660 1074 4333 2660 Fragments arranged by size: 4333 2660 1074 660 354 BanI G'GyrC_C Cuts at: 0 1383 7230 9081 Size: 1383 5847 1851 Fragments arranged by size: 5847 1851 1383 BanII G_rGCy'C Cuts at: 0 4686 7286 8289 9081 Size: 4686 2600 1003 792 Fragments arranged by size: 4686 2600 1003 792 BbsI GAAGACnn'nnnn_ Cuts at: 0 1162 2510 3449 5747 7513 8526 8875 9081 Size: 1162 1348 939 2298 1766 1013 349 206 Fragments arranged by size: 2298 1766 1348 1162 1013 939 349 206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1837 1871 7953 7987 9081 Size: 1837 34 6082 34 1094 Fragments arranged by size: 6082 1837 1094 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 5688 8598 9081 Size: 5688 2910 483 Fragments arranged by size: 5688 2910 483 BclI T'GATC_A Cuts at: 0 1425 5397 9081 Size: 1425 3972 3684 Fragments arranged by size: 3972 3684 1425 BfrBI ATG'CAT Cuts at: 0 94 482 1978 2435 3228 3705 4862 5298 Size: 94 388 1496 457 793 477 1157 436 Cuts at: 5298 6693 8641 9081 Size: 1395 1948 440 Fragments arranged by size: 1948 1496 1395 1157 793 477 457 440 436 388 94 BglII A'GATC_T Cuts at: 0 456 3205 9081 Size: 456 2749 5876 Fragments arranged by size: 5876 2749 456 BlpI GC'TnA_GC Cuts at: 0 7310 9081 Size: 7310 1771 Bme1580I G_kGCm'C Cuts at: 0 3156 3578 7092 9081 Size: 3156 422 3514 1989 Fragments arranged by size: 3514 3156 1989 422 BmgBI CAC'GTC Cuts at: 0 2076 3032 9081 Size: 2076 956 6049 Fragments arranged by size: 6049 2076 956 BmrI ACTGGGnnnn_n' Cuts at: 0 127 2087 2128 2234 6927 9081 Size: 127 1960 41 106 4693 2154 Fragments arranged by size: 4693 2154 1960 127 106 41 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2824 4705 5832 6549 9081 Size: 2824 1881 1127 717 2532 Fragments arranged by size: 2824 2532 1881 1127 717 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 608 2177 2738 2794 3018 3795 5874 7411 Size: 608 1569 561 56 224 777 2079 1537 Cuts at: 7411 7907 8963 9081 Size: 496 1056 118 Fragments arranged by size: 2079 1569 1537 1056 777 608 561 496 224 118 56 Bpu10I CC'TnA_GC Cuts at: 0 4448 4457 4533 7280 8483 9081 Size: 4448 9 76 2747 1203 598 Fragments arranged by size: 4448 2747 1203 598 76 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1377 2545 2609 3298 5164 8273 9081 Size: 1377 1168 64 689 1866 3109 808 Fragments arranged by size: 3109 1866 1377 1168 808 689 64 BsaI GGTCTCn'nnnn_ Cuts at: 0 4121 4145 9081 Size: 4121 24 4936 Fragments arranged by size: 4936 4121 24 BsaAI yAC'GTr Cuts at: 0 5236 9081 Size: 5236 3845 BsaBI GATnn'nnATC Cuts at: 0 1019 1286 7421 9081 Size: 1019 267 6135 1660 Fragments arranged by size: 6135 1660 1019 267 BsaHI Gr'CG_yC Cuts at: 0 5475 9081 Size: 5475 3606 BsaWI w'CCGG_w Cuts at: 0 1159 4835 9081 Size: 1159 3676 4246 Fragments arranged by size: 4246 3676 1159 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 37 67 4504 4534 7719 7749 8195 8225 Size: 37 30 4437 30 3185 30 446 30 Cuts at: 8225 8654 8684 8968 8998 9081 Size: 429 30 284 30 83 Fragments arranged by size: 4437 3185 446 429 284 83 37 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 978 2174 6535 7372 8216 8538 9081 Size: 978 1196 4361 837 844 322 543 Fragments arranged by size: 4361 1196 978 844 837 543 322 BseYI C'CCAG_C Cuts at: 0 556 1092 4060 9081 Size: 556 536 2968 5021 Fragments arranged by size: 5021 2968 556 536 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2117 9081 Size: 2117 6964 BsiHKAI G_wGCw'C Cuts at: 0 2952 3156 3775 4686 5358 5592 7092 7286 Size: 2952 204 619 911 672 234 1500 194 Cuts at: 7286 7496 8289 9081 Size: 210 793 792 Fragments arranged by size: 2952 1500 911 793 792 672 619 234 210 204 194 BsmI GAATG_Cn' Cuts at: 0 2044 2623 3895 4162 5055 9081 Size: 2044 579 1272 267 893 4026 Fragments arranged by size: 4026 2044 1272 893 579 267 BsmBI CGTCTCn'nnnn_ Cuts at: 0 5466 9081 Size: 5466 3615 Bsp1286I G_dGCh'C Cuts at: 0 2952 3156 3578 3775 4686 5358 5592 7092 Size: 2952 204 422 197 911 672 234 1500 Cuts at: 7092 7286 7496 8289 9081 Size: 194 210 793 792 Fragments arranged by size: 2952 1500 911 793 792 672 422 234 210 204 197 194 BspEI T'CCGG_A Cuts at: 0 4835 9081 Size: 4835 4246 BspHI T'CATG_A Cuts at: 0 4098 5301 7530 7983 9081 Size: 4098 1203 2229 453 1098 Fragments arranged by size: 4098 2229 1203 1098 453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 14 2813 7516 7678 8628 8656 9081 Size: 14 2799 4703 162 950 28 425 Fragments arranged by size: 4703 2799 950 425 162 28 14 BsrDI GCAATG_nn' Cuts at: 0 489 4055 4940 9081 Size: 489 3566 885 4141 Fragments arranged by size: 4141 3566 885 489 BsrGI T'GTAC_A Cuts at: 0 1173 6556 9081 Size: 1173 5383 2525 Fragments arranged by size: 5383 2525 1173 BssSI C'ACGA_G Cuts at: 0 8099 9081 Size: 8099 982 BstAPI GCAn_nnn'nTGC Cuts at: 0 15 2947 7491 7693 9081 Size: 15 2932 4544 202 1388 Fragments arranged by size: 4544 2932 1388 202 15 BstXI CCAn_nnnn'nTGG Cuts at: 0 6878 8659 9081 Size: 6878 1781 422 Fragments arranged by size: 6878 1781 422 BstYI r'GATC_y Cuts at: 0 354 456 1014 2088 2448 2762 3205 4002 Size: 354 102 558 1074 360 314 443 797 Cuts at: 4002 5797 6421 8836 9081 Size: 1795 624 2415 245 Fragments arranged by size: 2415 1795 1074 797 624 558 443 360 354 314 245 102 Bsu36I CC'TnA_GG Cuts at: 0 4111 9081 Size: 4111 4970 BtgI C'CryG_G Cuts at: 0 56 1214 1493 6426 6996 7629 8173 9081 Size: 56 1158 279 4933 570 633 544 908 Fragments arranged by size: 4933 1158 908 633 570 544 279 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 7492 9081 Size: 7492 1589 BtsI GCAGTG_nn' Cuts at: 0 45 551 1042 2952 5579 6980 8509 9081 Size: 45 506 491 1910 2627 1401 1529 572 Fragments arranged by size: 2627 1910 1529 1401 572 506 491 45 ClaI AT'CG_AT Cuts at: 0 5255 9081 Size: 5255 3826 DraI TTT'AAA Cuts at: 0 446 1763 5045 5597 6050 6101 9044 9081 Size: 446 1317 3282 552 453 51 2943 37 Fragments arranged by size: 3282 2943 1317 552 453 446 51 37 DraIII CAC_nnn'GTG Cuts at: 0 15 9081 Size: 15 9066 EaeI y'GGCC_r Cuts at: 0 312 3076 4470 6880 7004 8018 9081 Size: 312 2764 1394 2410 124 1014 1063 Fragments arranged by size: 2764 2410 1394 1063 1014 312 124 EarI CTCTTCn'nnn_ Cuts at: 0 1145 2195 6212 6994 7332 7592 7667 7768 Size: 1145 1050 4017 782 338 260 75 101 Cuts at: 7768 8270 8813 9081 Size: 502 543 268 Fragments arranged by size: 4017 1145 1050 782 543 502 338 268 260 101 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 621 9081 Size: 621 8460 EcoICRI GAG'CTC Cuts at: 0 4684 7284 8287 9081 Size: 4684 2600 1003 794 Fragments arranged by size: 4684 2600 1003 794 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 608 2177 2738 2794 3018 3085 3795 Size: 452 156 1569 561 56 224 67 710 Cuts at: 3795 3890 5789 5874 6193 6260 6320 7411 7907 Size: 95 1899 85 319 67 60 1091 496 Cuts at: 7907 8837 8963 9081 Size: 930 126 118 Fragments arranged by size: 1899 1569 1091 930 710 561 496 452 319 224 156 126 118 95 85 67 67 60 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 10 9081 Size: 10 9071 EcoO109I rG'GnC_Cy Cuts at: 0 3273 7690 8965 9081 Size: 3273 4417 1275 116 Fragments arranged by size: 4417 3273 1275 116 EcoRI G'AATT_C Cuts at: 0 1586 6912 8012 9081 Size: 1586 5326 1100 1069 Fragments arranged by size: 5326 1586 1100 1069 EcoRV GAT'ATC Cuts at: 0 997 1799 6666 7882 7923 9081 Size: 997 802 4867 1216 41 1158 Fragments arranged by size: 4867 1216 1158 997 802 41 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3115 4050 8267 9081 Size: 3115 935 4217 814 Fragments arranged by size: 4217 3115 935 814 HaeII r_GCGC'y Cuts at: 0 81 9081 Size: 81 9000 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1288 1320 1603 1635 2172 2204 2754 2786 Size: 1288 32 283 32 537 32 550 32 Cuts at: 2786 3007 3039 3418 3450 3951 3983 4350 4382 Size: 221 32 379 32 501 32 367 32 Cuts at: 4382 4504 4536 5812 5844 6214 6246 6350 6382 Size: 122 32 1276 32 370 32 104 32 Cuts at: 6382 7500 7532 7610 7642 7666 7698 8241 8271 Size: 1118 32 78 32 24 32 543 30 Cuts at: 8271 8273 8303 8850 8882 9081 Size: 2 30 547 32 199 Fragments arranged by size: 1288 1276 1118 550 547 543 537 501 379 370 367 283 221 199 122 104 78 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 30 24 2 HincII GTy'rAC Cuts at: 0 258 767 1665 2073 2130 2410 2828 8749 Size: 258 509 898 408 57 280 418 5921 Cuts at: 8749 9081 Size: 332 Fragments arranged by size: 5921 898 509 418 408 332 280 258 57 HindIII A'AGCT_T Cuts at: 0 6837 7712 9081 Size: 6837 875 1369 Fragments arranged by size: 6837 1369 875 HpaI GTT'AAC Cuts at: 0 258 9081 Size: 258 8823 KpnI G_GTAC'C Cuts at: 0 1387 9081 Size: 1387 7694 MfeI C'AATT_G Cuts at: 0 4544 5129 7486 9081 Size: 4544 585 2357 1595 Fragments arranged by size: 4544 2357 1595 585 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 940 4456 5714 5969 7638 8284 8836 9081 Size: 940 3516 1258 255 1669 646 552 245 Fragments arranged by size: 3516 1669 1258 940 646 552 255 245 MscI TGG'CCA Cuts at: 0 314 3078 4472 6882 7006 8020 9081 Size: 314 2764 1394 2410 124 1014 1061 Fragments arranged by size: 2764 2410 1394 1061 1014 314 124 MslI CAynn'nnrTG Cuts at: 0 99 1052 1639 1720 1811 1919 1973 3296 Size: 99 953 587 81 91 108 54 1323 Cuts at: 3296 3679 4987 5293 5509 6973 8099 8657 9081 Size: 383 1308 306 216 1464 1126 558 424 Fragments arranged by size: 1464 1323 1308 1126 953 587 558 424 383 306 216 108 99 91 81 54 MspA1I CmG'CkG Cuts at: 0 613 1417 8116 8566 9081 Size: 613 804 6699 450 515 Fragments arranged by size: 6699 804 613 515 450 NcoI C'CATG_G Cuts at: 0 1214 1493 6426 6996 7629 8173 9081 Size: 1214 279 4933 570 633 544 908 Fragments arranged by size: 4933 1214 908 633 570 544 279 NdeI CA'TA_TG Cuts at: 0 4370 5510 8679 8730 9081 Size: 4370 1140 3169 51 351 Fragments arranged by size: 4370 3169 1140 351 51 NsiI A_TGCA'T Cuts at: 0 96 484 1980 2437 3230 3707 4864 5300 Size: 96 388 1496 457 793 477 1157 436 Cuts at: 5300 6695 8643 9081 Size: 1395 1948 438 Fragments arranged by size: 1948 1496 1395 1157 793 477 457 438 436 388 96 NspI r_CATG'y Cuts at: 0 1051 1522 1810 2282 2652 2945 3232 3372 Size: 1051 471 288 472 370 293 287 140 Cuts at: 3372 3709 4456 4767 6098 7096 8098 8172 8481 Size: 337 747 311 1331 998 1002 74 309 Cuts at: 8481 8911 9081 Size: 430 170 Fragments arranged by size: 1331 1051 1002 998 747 472 471 430 370 337 311 309 293 288 287 170 140 74 PciI A'CATG_T Cuts at: 0 2648 3368 4763 7092 9081 Size: 2648 720 1395 2329 1989 Fragments arranged by size: 2648 2329 1989 1395 720 PflMI CCAn_nnn'nTGG Cuts at: 0 7002 8179 8470 9081 Size: 7002 1177 291 611 Fragments arranged by size: 7002 1177 611 291 PfoI T'CCnGG_A Cuts at: 0 3665 7427 7884 9081 Size: 3665 3762 457 1197 Fragments arranged by size: 3762 3665 1197 457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 5445 5477 9081 Size: 5445 32 3604 Fragments arranged by size: 5445 3604 32 PpuMI rG'GwC_Cy Cuts at: 0 3273 7690 9081 Size: 3273 4417 1391 Fragments arranged by size: 4417 3273 1391 PshAI GACnn'nnGTC Cuts at: 0 8744 9081 Size: 8744 337 PsiI TTA'TAA Cuts at: 0 3182 3751 9081 Size: 3182 569 5330 Fragments arranged by size: 5330 3182 569 PspXI vC'TCGA_Gb Cuts at: 0 692 9081 Size: 692 8389 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3798 3830 5694 5726 9081 Size: 3798 32 1864 32 3355 Fragments arranged by size: 3798 3355 1864 32 32 PstI C_TGCA'G Cuts at: 0 850 5094 5217 7689 9081 Size: 850 4244 123 2472 1392 Fragments arranged by size: 4244 2472 1392 850 123 PvuII CAG'CTG Cuts at: 0 613 1417 8116 8566 9081 Size: 613 804 6699 450 515 Fragments arranged by size: 6699 804 613 515 450 SacI G_AGCT'C Cuts at: 0 4686 7286 8289 9081 Size: 4686 2600 1003 792 Fragments arranged by size: 4686 2600 1003 792 SapI GCTCTTCn'nnn_ Cuts at: 0 6212 9081 Size: 6212 2869 ScaI AGT'ACT Cuts at: 0 1442 6803 8824 9081 Size: 1442 5361 2021 257 Fragments arranged by size: 5361 2021 1442 257 SexAI A'CCwGG_T Cuts at: 0 11 3126 9081 Size: 11 3115 5955 Fragments arranged by size: 5955 3115 11 SfcI C'TryA_G Cuts at: 0 846 3137 3464 3623 5090 5213 5423 6247 Size: 846 2291 327 159 1467 123 210 824 Cuts at: 6247 6753 7685 8561 9081 Size: 506 932 876 520 Fragments arranged by size: 2291 1467 932 876 846 824 520 506 327 210 159 123 SmlI C'TyrA_G Cuts at: 0 692 1392 2524 2624 3277 5143 8288 9081 Size: 692 700 1132 100 653 1866 3145 793 Fragments arranged by size: 3145 1866 1132 793 700 692 653 100 SnaBI TAC'GTA Cuts at: 0 5236 9081 Size: 5236 3845 SpeI A'CTAG_T Cuts at: 0 5698 7143 9081 Size: 5698 1445 1938 Fragments arranged by size: 5698 1938 1445 SphI G_CATG'C Cuts at: 0 1051 2282 2945 4456 8481 9081 Size: 1051 1231 663 1511 4025 600 Fragments arranged by size: 4025 1511 1231 1051 663 600 SspI AAT'ATT Cuts at: 0 171 2031 8934 9081 Size: 171 1860 6903 147 Fragments arranged by size: 6903 1860 171 147 StuI AGG'CCT Cuts at: 0 3135 4703 9081 Size: 3135 1568 4378 Fragments arranged by size: 4378 3135 1568 StyI C'CwwG_G Cuts at: 0 651 739 1214 1493 2538 4025 4590 4698 Size: 651 88 475 279 1045 1487 565 108 Cuts at: 4698 6426 6996 7629 8173 9081 Size: 1728 570 633 544 908 Fragments arranged by size: 1728 1487 1045 908 651 633 570 565 544 475 279 108 88 SwaI ATTT'AAAT Cuts at: 0 446 9081 Size: 446 8635 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2242 4807 9081 Size: 2242 2565 4274 Fragments arranged by size: 4274 2565 2242 TatI w'GTAC_w Cuts at: 0 759 1173 1440 1563 3591 3948 4424 6556 Size: 759 414 267 123 2028 357 476 2132 Cuts at: 6556 6801 8185 8822 9081 Size: 245 1384 637 259 Fragments arranged by size: 2132 2028 1384 759 637 476 414 357 267 259 245 123 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 37 69 1089 1121 5011 5043 8195 8227 Size: 37 32 1020 32 3890 32 3152 32 Cuts at: 8227 8930 8962 8968 9000 9081 Size: 703 32 6 32 81 Fragments arranged by size: 3890 3152 1020 703 81 37 32 32 32 32 32 32 6 XbaI T'CTAG_A Cuts at: 0 3436 9081 Size: 3436 5645 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 414 2247 7755 8495 9081 Size: 414 1833 5508 740 586 Fragments arranged by size: 5508 1833 740 586 414 XhoI C'TCGA_G Cuts at: 0 692 9081 Size: 692 8389 XmnI GAAnn'nnTTC Cuts at: 0 897 914 1442 5256 6344 7604 7808 8250 Size: 897 17 528 3814 1088 1260 204 442 Cuts at: 8250 9081 Size: 831 Fragments arranged by size: 3814 1260 1088 897 831 528 442 204 17 ZraI GAC'GTC Cuts at: 0 5476 9081 Size: 5476 3605 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI PfoI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuII SacI SapI ScaI SexAI SfcI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: AflII AgeI AhdI AloI ApaI AscI AsiSI BbeI BbvCI BglI BmtI BsiEI BsiWI BsrBI BsrFI BssHII BstBI BstEII BstZ17I CspCI DrdI EagI FseI FspI FspAI KasI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PspOMI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SrfI Tth111I XmaI