Restriction map for
pF1KB7006
(Linear) (Six-base) MAPSORT of: KIBB7006.seq Check: 2051 from: 1 to: 3198
KIBB7006.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
Acc65I G'GTAC_C
Cuts at: 0 2824 3191 3198
Size: 2824 367 7
Fragments arranged by size:
2824 367 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 809 1259 2159 2618 3143 3198
Size: 268 541 450 900 459 525 55
Fragments arranged by size:
900 541 525 459 450 268 55
AgeI A'CCGG_T
Cuts at: 0 1038 3198
Size: 1038 2160
AleI CACnn'nnGTG
Cuts at: 0 1503 3198
Size: 1503 1695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1166 2797 3198
Size: 1166 1631 401
Fragments arranged by size:
1631 1166 401
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2317 2349 2635 2667 3198
Size: 2317 32 286 32 531
Fragments arranged by size:
2317 531 286 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 307 1077 1416 2244 3154 3198
Size: 307 770 339 828 910 44
Fragments arranged by size:
910 828 770 339 307 44
ApaLI G'TGCA_C
Cuts at: 0 2913 3198
Size: 2913 285
ApoI r'AATT_y
Cuts at: 0 419 2316 3140 3198
Size: 419 1897 824 58
Fragments arranged by size:
1897 824 419 58
AseI AT'TA_AT
Cuts at: 0 722 3198
Size: 722 2476
AvaI C'yCGr_G
Cuts at: 0 311 1376 2003 3198
Size: 311 1065 627 1195
Fragments arranged by size:
1195 1065 627 311
AvrII C'CTAG_G
Cuts at: 0 2617 3198
Size: 2617 581
BamHI G'GATC_C
Cuts at: 0 1030 2285 2325 2468 3198
Size: 1030 1255 40 143 730
Fragments arranged by size:
1255 1030 730 143 40
BanI G'GyrC_C
Cuts at: 0 1842 2824 3191 3198
Size: 1842 982 367 7
Fragments arranged by size:
1842 982 367 7
BanII G_rGCy'C
Cuts at: 0 545 3198
Size: 545 2653
BbeI G_GCGC'C
Cuts at: 0 1846 3198
Size: 1846 1352
BbsI GAAGACnn'nnnn_
Cuts at: 0 1353 2002 3198
Size: 1353 649 1196
Fragments arranged by size:
1353 1196 649
BbvCI CC'TCA_GC
Cuts at: 0 386 3198
Size: 386 2812
BclI T'GATC_A
Cuts at: 0 996 3198
Size: 996 2202
BglI GCCn_nnn'nGGC
Cuts at: 0 142 1611 1663 1936 3198
Size: 142 1469 52 273 1262
Fragments arranged by size:
1469 1262 273 142 52
BglII A'GATC_T
Cuts at: 0 56 902 1338 1617 3198
Size: 56 846 436 279 1581
Fragments arranged by size:
1581 846 436 279 56
BlpI GC'TnA_GC
Cuts at: 0 1058 3198
Size: 1058 2140
Bme1580I G_kGCm'C
Cuts at: 0 2838 2917 3198
Size: 2838 79 281
Fragments arranged by size:
2838 281 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1352 3198
Size: 58 1294 1846
Fragments arranged by size:
1846 1294 58
Bpu10I CC'TnA_GC
Cuts at: 0 386 2657 3198
Size: 386 2271 541
Fragments arranged by size:
2271 541 386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 3198
Size: 1498 1700
BsaI GGTCTCn'nnnn_
Cuts at: 0 2031 2080 3198
Size: 2031 49 1118
Fragments arranged by size:
2031 1118 49
BsaHI Gr'CG_yC
Cuts at: 0 1843 3198
Size: 1843 1355
BsaWI w'CCGG_w
Cuts at: 0 1038 1203 2282 3198
Size: 1038 165 1079 916
Fragments arranged by size:
1079 1038 916 165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1794 1824 1830 1860 3198
Size: 1794 30 6 30 1338
Fragments arranged by size:
1794 1338 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 2567 3055 3198
Size: 97 2470 488 143
Fragments arranged by size:
2470 488 143 97
BseYI C'CCAG_C
Cuts at: 0 1658 2251 2978 3015 3170 3198
Size: 1658 593 727 37 155 28
Fragments arranged by size:
1658 727 593 155 37 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 959 3198
Size: 160 799 2239
Fragments arranged by size:
2239 799 160
BsiEI CG_ry'CG
Cuts at: 0 26 149 1931 3198
Size: 26 123 1782 1267
Fragments arranged by size:
1782 1267 123 26
BsiHKAI G_wGCw'C
Cuts at: 0 545 560 1088 1713 2917 3198
Size: 545 15 528 625 1204 281
Fragments arranged by size:
1204 625 545 528 281 15
BsmI GAATG_Cn'
Cuts at: 0 252 1979 3198
Size: 252 1727 1219
Fragments arranged by size:
1727 1219 252
Bsp1286I G_dGCh'C
Cuts at: 0 545 560 1088 1713 2838 2917 3198
Size: 545 15 528 625 1125 79 281
Fragments arranged by size:
1125 625 545 528 281 79 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 371 1053 1424 3198
Size: 371 682 371 1774
Fragments arranged by size:
1774 682 371 371
BsrDI GCAATG_nn'
Cuts at: 0 1148 2754 2756 3198
Size: 1148 1606 2 442
Fragments arranged by size:
1606 1148 442 2
BsrFI r'CCGG_y
Cuts at: 0 42 1038 1455 3198
Size: 42 996 417 1743
Fragments arranged by size:
1743 996 417 42
BsrGI T'GTAC_A
Cuts at: 0 1470 3198
Size: 1470 1728
BssSI C'ACGA_G
Cuts at: 0 79 1706 3198
Size: 79 1627 1492
Fragments arranged by size:
1627 1492 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1416 3198
Size: 1416 1782
BstBI TT'CG_AA
Cuts at: 0 3144 3198
Size: 3144 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1395 2775 3015 3198
Size: 1395 1380 240 183
Fragments arranged by size:
1395 1380 240 183
BstYI r'GATC_y
Cuts at: 0 56 902 1030 1188 1338 1617 1743 2285
Size: 56 846 128 158 150 279 126 542
Cuts at: 2285 2325 2468 3198
Size: 40 143 730
Fragments arranged by size:
846 730 542 279 158 150 143 128
126 56 40
Bsu36I CC'TnA_GG
Cuts at: 0 1700 2510 3198
Size: 1700 810 688
Fragments arranged by size:
1700 810 688
BtgI C'CryG_G
Cuts at: 0 128 1400 1533 1838 3198
Size: 128 1272 133 305 1360
Fragments arranged by size:
1360 1272 305 133 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2844 3198
Size: 2844 354
BtsI GCAGTG_nn'
Cuts at: 0 1835 2826 2917 3163 3198
Size: 1835 991 91 246 35
Fragments arranged by size:
1835 991 246 91 35
ClaI AT'CG_AT
Cuts at: 0 1163 2108 3198
Size: 1163 945 1090
Fragments arranged by size:
1163 1090 945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2390 2425 3198
Size: 2390 35 773
Fragments arranged by size:
2390 773 35
DraI TTT'AAA
Cuts at: 0 680 3198
Size: 680 2518
DraIII CAC_nnn'GTG
Cuts at: 0 69 564 3198
Size: 69 495 2634
Fragments arranged by size:
2634 495 69
DrdI GACnn_nn'nnGTC
Cuts at: 0 1537 3198
Size: 1537 1661
EaeI y'GGCC_r
Cuts at: 0 131 146 512 1043 1385 1397 1442 1457
Size: 131 15 366 531 342 12 45 15
Cuts at: 1457 1568 1928 2222 2483 2891 3198
Size: 111 360 294 261 408 307
Fragments arranged by size:
531 408 366 360 342 307 294 261
131 111 45 15 15 12
EagI C'GGCC_G
Cuts at: 0 146 1928 3198
Size: 146 1782 1270
Fragments arranged by size:
1782 1270 146
EarI CTCTTCn'nnn_
Cuts at: 0 205 793 1208 1862 2642 3198
Size: 205 588 415 654 780 556
Fragments arranged by size:
780 654 588 556 415 205
EcoICRI GAG'CTC
Cuts at: 0 543 3198
Size: 543 2655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 268 809 1259 1352 2159 2618 3143
Size: 58 210 541 450 93 807 459 525
Cuts at: 3143 3198
Size: 55
Fragments arranged by size:
807 541 525 459 450 210 93 58
55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2944 3198
Size: 2944 254
EcoO109I rG'GnC_Cy
Cuts at: 0 1603 2607 3198
Size: 1603 1004 591
Fragments arranged by size:
1603 1004 591
EcoRI G'AATT_C
Cuts at: 0 2316 3198
Size: 2316 882
EcoRV GAT'ATC
Cuts at: 0 2464 3198
Size: 2464 734
HaeII r_GCGC'y
Cuts at: 0 1846 3198
Size: 1846 1352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 981 1013 1500 1532 2143 2175
Size: 283 32 666 32 487 32 611 32
Cuts at: 2175 2453 2485 3028 3060 3198
Size: 278 32 543 32 138
Fragments arranged by size:
666 611 543 487 283 278 138 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 259 674 1915 3198
Size: 259 415 1241 1283
Fragments arranged by size:
1283 1241 415 259
KasI G'GCGC_C
Cuts at: 0 1842 3198
Size: 1842 1356
KpnI G_GTAC'C
Cuts at: 0 2828 3195 3198
Size: 2828 367 3
Fragments arranged by size:
2828 367 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 2147 2450 2529 2580 3198
Size: 150 1997 303 79 51 618
Fragments arranged by size:
1997 618 303 150 79 51
MscI TGG'CCA
Cuts at: 0 514 1045 1387 1399 1444 2224 2485 2893
Size: 514 531 342 12 45 780 261 408
Cuts at: 2893 3198
Size: 305
Fragments arranged by size:
780 531 514 408 342 305 261 45
12
MslI CAynn'nnrTG
Cuts at: 0 1503 3061 3198
Size: 1503 1558 137
Fragments arranged by size:
1558 1503 137
MspA1I CmG'CkG
Cuts at: 0 1468 1503 1650 1662 3198
Size: 1468 35 147 12 1536
Fragments arranged by size:
1536 1468 147 35 12
NarI GG'CG_CC
Cuts at: 0 1843 3198
Size: 1843 1355
PasI CC'CwG_GG
Cuts at: 0 871 3198
Size: 871 2327
PflMI CCAn_nnn'nTGG
Cuts at: 0 845 1198 3198
Size: 845 353 2000
Fragments arranged by size:
2000 845 353
PfoI T'CCnGG_A
Cuts at: 0 35 2450 3198
Size: 35 2415 748
Fragments arranged by size:
2415 748 35
PpuMI rG'GwC_Cy
Cuts at: 0 2607 3198
Size: 2607 591
PspXI vC'TCGA_Gb
Cuts at: 0 1376 3198
Size: 1376 1822
PstI C_TGCA'G
Cuts at: 0 1412 1421 3198
Size: 1412 9 1777
Fragments arranged by size:
1777 1412 9
PvuI CG_AT'CG
Cuts at: 0 26 3198
Size: 26 3172
PvuII CAG'CTG
Cuts at: 0 1468 1650 1662 3198
Size: 1468 182 12 1536
Fragments arranged by size:
1536 1468 182 12
SacI G_AGCT'C
Cuts at: 0 545 3198
Size: 545 2653
SapI GCTCTTCn'nnn_
Cuts at: 0 1208 3198
Size: 1208 1990
SexAI A'CCwGG_T
Cuts at: 0 65 3198
Size: 65 3133
SfcI C'TryA_G
Cuts at: 0 698 1408 1417 3198
Size: 698 710 9 1781
Fragments arranged by size:
1781 710 698 9
SfoI GGC'GCC
Cuts at: 0 1844 3198
Size: 1844 1354
SgrAI Cr'CCGG_yG
Cuts at: 0 1038 3198
Size: 1038 2160
SmlI C'TyrA_G
Cuts at: 0 311 1376 1477 3198
Size: 311 1065 101 1721
Fragments arranged by size:
1721 1065 311 101
StuI AGG'CCT
Cuts at: 0 1987 2622 2869 3198
Size: 1987 635 247 329
Fragments arranged by size:
1987 635 329 247
StyI C'CwwG_G
Cuts at: 0 660 1302 1382 2486 2617 2852 2864 2894
Size: 660 642 80 1104 131 235 12 30
Cuts at: 2894 3198
Size: 304
Fragments arranged by size:
1104 660 642 304 235 131 80 30
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1531 1652 2992 3198
Size: 1531 121 1340 206
Fragments arranged by size:
1531 1340 206 121
TatI w'GTAC_w
Cuts at: 0 1470 3198
Size: 1470 1728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1830 1862 3198
Size: 1830 32 1336
Fragments arranged by size:
1830 1336 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1437 1821 2163 2385 3063 3198
Size: 1437 384 342 222 678 135
Fragments arranged by size:
1437 678 384 342 222 135
XhoI C'TCGA_G
Cuts at: 0 311 1376 3198
Size: 311 1065 1822
Fragments arranged by size:
1822 1065 311
XmnI GAAnn'nnTTC
Cuts at: 0 1979 2421 3198
Size: 1979 442 777
Fragments arranged by size:
1979 777 442
Enzymes that do cut:
Acc65I AcuI AgeI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BclI BglI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI
MspA1I NarI PasI PflMI PfoI PpuMI PspXI PstI
PvuI PvuII SacI SapI SexAI SfcI SfoI SgrAI
SmlI StuI StyI TaqII TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
ApaI AscI AsiSI BaeI BcgI BciVI BfrBI BmgBI
BmrI BmtI BplI BsaAI BsaBI BsiWI BsmBI BspEI
BspHI BsrBI BssHII BstEII BstZ17I EciI FalI FseI
FspI FspAI HincII HpaI MfeI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XmaI ZraI