Restriction map for
pF1KB7009
(Linear) (Six-base) MAPSORT of: KIBB7009.seq Check: 2006 from: 1 to: 3216
KIBB7009.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1492 3216
Size: 1492 1724
AccI GT'mk_AC
Cuts at: 0 841 1949 3216
Size: 841 1108 1267
Fragments arranged by size:
1267 1108 841
Acc65I G'GTAC_C
Cuts at: 0 790 3180 3216
Size: 790 2390 36
Fragments arranged by size:
2390 790 36
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 2575 2809 3216
Size: 1177 1398 234 407
Fragments arranged by size:
1398 1177 407 234
AfeI AGC'GCT
Cuts at: 0 3094 3216
Size: 3094 122
AleI CACnn'nnGTG
Cuts at: 0 1552 1805 3216
Size: 1552 253 1411
Fragments arranged by size:
1552 1411 253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 452 935 1198 1220 1570 3216
Size: 452 483 263 22 350 1646
Fragments arranged by size:
1646 483 452 350 263 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 996 1028 2368 2400 3216
Size: 996 32 1340 32 816
Fragments arranged by size:
1340 996 816 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 519 705 771 1099 1415 1926 2139 2699
Size: 519 186 66 328 316 511 213 560
Cuts at: 2699 2832 2893 3168 3216
Size: 133 61 275 48
Fragments arranged by size:
560 519 511 328 316 275 213 186
133 66 61 48
ApaI G_GGCC'C
Cuts at: 0 1382 1824 2115 2883 3216
Size: 1382 442 291 768 333
Fragments arranged by size:
1382 768 442 333 291
ApaLI G'TGCA_C
Cuts at: 0 2154 3216
Size: 2154 1062
ApoI r'AATT_y
Cuts at: 0 571 3216
Size: 571 2645
AvaI C'yCGr_G
Cuts at: 0 977 1163 1266 1542 2642 2780 2882 3216
Size: 977 186 103 276 1100 138 102 334
Fragments arranged by size:
1100 977 334 276 186 138 103 102
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 270 303 3216
Size: 270 33 2913
Fragments arranged by size:
2913 270 33
BamHI G'GATC_C
Cuts at: 0 3132 3216
Size: 3132 84
BanI G'GyrC_C
Cuts at: 0 790 1504 1554 1639 3180 3216
Size: 790 714 50 85 1541 36
Fragments arranged by size:
1541 790 714 85 50 36
BanII G_rGCy'C
Cuts at: 0 194 479 805 1273 1382 1748 1824 1856
Size: 194 285 326 468 109 366 76 32
Cuts at: 1856 1892 2115 2883 2942 3216
Size: 36 223 768 59 274
Fragments arranged by size:
768 468 366 326 285 274 223 194
109 76 59 36 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 3216
Size: 542 2674
BbvCI CC'TCA_GC
Cuts at: 0 266 819 3216
Size: 266 553 2397
Fragments arranged by size:
2397 553 266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 831 865 2494 2528 2633 2667 3216
Size: 831 34 1629 34 105 34 549
Fragments arranged by size:
1629 831 549 105 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3208 3216
Size: 3208 8
BglII A'GATC_T
Cuts at: 0 1520 3216
Size: 1520 1696
BlpI GC'TnA_GC
Cuts at: 0 478 1607 2275 3216
Size: 478 1129 668 941
Fragments arranged by size:
1129 941 668 478
Bme1580I G_kGCm'C
Cuts at: 0 1382 1401 1549 1824 2115 2158 2233 2400
Size: 1382 19 148 275 291 43 75 167
Cuts at: 2400 2883 3216
Size: 483 333
Fragments arranged by size:
1382 483 333 291 275 167 148 75
43 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 800 831 1403 2394 2448 2667 3216
Size: 800 31 572 991 54 219 549
Fragments arranged by size:
991 800 572 549 219 54 31
BmtI G_CTAG'C
Cuts at: 0 133 3216
Size: 133 3083
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1542 2806 3216
Size: 1542 1264 410
Fragments arranged by size:
1542 1264 410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 256 541 1046 1450 1615 1732 1768
Size: 112 144 285 505 404 165 117 36
Cuts at: 1768 2458 2855 2915 2954 3097 3146 3216
Size: 690 397 60 39 143 49 70
Fragments arranged by size:
690 505 404 397 285 165 144 143
117 112 70 60 49 39 36
Bpu10I CC'TnA_GC
Cuts at: 0 266 799 819 1886 2941 3216
Size: 266 533 20 1067 1055 275
Fragments arranged by size:
1067 1055 533 275 266 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 1784 3216
Size: 1784 1432
BsaAI yAC'GTr
Cuts at: 0 3105 3216
Size: 3105 111
BsaHI Gr'CG_yC
Cuts at: 0 155 1389 3216
Size: 155 1234 1827
Fragments arranged by size:
1827 1234 155
BsaWI w'CCGG_w
Cuts at: 0 1298 2126 3216
Size: 1298 828 1090
Fragments arranged by size:
1298 1090 828
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 1735 1765 1796 1826 2853 2883
Size: 514 30 1191 30 31 30 1027 30
Cuts at: 2883 3216
Size: 333
Fragments arranged by size:
1191 1027 514 333 31 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 58 78 391 430 1531 1723 1786
Size: 31 27 20 313 39 1101 192 63
Cuts at: 1786 2512 2660 3216
Size: 726 148 556
Fragments arranged by size:
1101 726 556 313 192 148 63 39
31 27 20
BseYI C'CCAG_C
Cuts at: 0 1136 1144 1277 1635 1960 1964 2108 2875
Size: 1136 8 133 358 325 4 144 767
Cuts at: 2875 3048 3113 3216
Size: 173 65 103
Fragments arranged by size:
1136 767 358 325 173 144 133 103
65 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 664 1759 2749 3085 3216
Size: 355 309 1095 990 336 131
Fragments arranged by size:
1095 990 355 336 309 131
BsiEI CG_ry'CG
Cuts at: 0 106 249 3216
Size: 106 143 2967
Fragments arranged by size:
2967 143 106
BsiHKAI G_wGCw'C
Cuts at: 0 431 479 753 900 956 1748 2158 2407
Size: 431 48 274 147 56 792 410 249
Cuts at: 2407 3216
Size: 809
Fragments arranged by size:
809 792 431 410 274 249 147 56
48
BsiWI C'GTAC_G
Cuts at: 0 2630 3105 3216
Size: 2630 475 111
Fragments arranged by size:
2630 475 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3073 3216
Size: 3073 143
Bsp1286I G_dGCh'C
Cuts at: 0 194 431 479 753 805 900 956 1273
Size: 194 237 48 274 52 95 56 317
Cuts at: 1273 1382 1401 1549 1748 1824 1856 1892 2115
Size: 109 19 148 199 76 32 36 223
Cuts at: 2115 2158 2233 2400 2407 2883 2942 3216
Size: 43 75 167 7 476 59 274
Fragments arranged by size:
476 317 274 274 237 223 199 194
167 148 109 95 76 75 59 56
52 48 43 36 32 19 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 822 1492 2807 3107 3216
Size: 152 670 670 1315 300 109
Fragments arranged by size:
1315 670 670 300 152 109
BsrBI CCG'CTC
Cuts at: 0 21 3216
Size: 21 3195
BsrDI GCAATG_nn'
Cuts at: 0 2588 3216
Size: 2588 628
BsrFI r'CCGG_y
Cuts at: 0 2983 3216
Size: 2983 233
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1327 1569 2609 2699 3216
Size: 1327 242 1040 90 517
Fragments arranged by size:
1327 1040 517 242 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1024 1152 1353 3216
Size: 1024 128 201 1863
Fragments arranged by size:
1863 1024 201 128
BstYI r'GATC_y
Cuts at: 0 1422 1520 3132 3216
Size: 1422 98 1612 84
Fragments arranged by size:
1612 1422 98 84
Bsu36I CC'TnA_GG
Cuts at: 0 61 1769 3216
Size: 61 1708 1447
Fragments arranged by size:
1708 1447 61
BtgI C'CryG_G
Cuts at: 0 218 732 829 3216
Size: 218 514 97 2387
Fragments arranged by size:
2387 514 218 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2178 3216
Size: 2178 1038
BtsI GCAGTG_nn'
Cuts at: 0 676 681 956 1570 1990 3216
Size: 676 5 275 614 420 1226
Fragments arranged by size:
1226 676 614 420 275 5
DrdI GACnn_nn'nnGTC
Cuts at: 0 2848 3216
Size: 2848 368
EaeI y'GGCC_r
Cuts at: 0 103 246 2241 2769 3083 3216
Size: 103 143 1995 528 314 133
Fragments arranged by size:
1995 528 314 143 133 103
EagI C'GGCC_G
Cuts at: 0 103 246 3216
Size: 103 143 2970
Fragments arranged by size:
2970 143 103
EarI CTCTTCn'nnn_
Cuts at: 0 8 2797 3065 3170 3216
Size: 8 2789 268 105 46
Fragments arranged by size:
2789 268 105 46 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 751 1849 2167 3216
Size: 751 1098 318 1049
Fragments arranged by size:
1098 1049 751 318
EcoICRI GAG'CTC
Cuts at: 0 477 1746 3216
Size: 477 1269 1470
Fragments arranged by size:
1470 1269 477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 256 541 1046 1177 1450 1615 1732
Size: 112 144 285 505 131 273 165 117
Cuts at: 1732 1768 2458 2575 2809 2855 2915 2954 3097
Size: 36 690 117 234 46 60 39 143
Cuts at: 3097 3146 3216
Size: 49 70
Fragments arranged by size:
690 505 285 273 234 165 144 143
131 117 117 112 70 60 49 46
39 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1195 2992 3216
Size: 1195 1797 224
Fragments arranged by size:
1797 1195 224
EcoO109I rG'GnC_Cy
Cuts at: 0 702 780 1012 1363 1379 1626 1821 2112
Size: 702 78 232 351 16 247 195 291
Cuts at: 2112 2136 2376 2574 2879 3216
Size: 24 240 198 305 337
Fragments arranged by size:
702 351 337 305 291 247 240 232
198 195 78 24 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3031 3216
Size: 3031 185
HaeII r_GCGC'y
Cuts at: 0 59 1440 1842 1869 2490 3096 3216
Size: 59 1381 402 27 621 606 120
Fragments arranged by size:
1381 621 606 402 120 59 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1735 1762 1767 1794 3182 3214 3216
Size: 1735 27 5 27 1388 32 2
Fragments arranged by size:
1735 1388 32 27 27 5 2
HincII GTy'rAC
Cuts at: 0 842 3216
Size: 842 2374
HindIII A'AGCT_T
Cuts at: 0 2951 3216
Size: 2951 265
KpnI G_GTAC'C
Cuts at: 0 794 3184 3216
Size: 794 2390 32
Fragments arranged by size:
2390 794 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 2494 3216
Size: 512 1982 722
Fragments arranged by size:
1982 722 512
MslI CAynn'nnrTG
Cuts at: 0 1552 1805 2221 3216
Size: 1552 253 416 995
Fragments arranged by size:
1552 995 416 253
MspA1I CmG'CkG
Cuts at: 0 150 173 270 528 1096 1964 2436 2696
Size: 150 23 97 258 568 868 472 260
Cuts at: 2696 2875 2974 3216
Size: 179 99 242
Fragments arranged by size:
868 568 472 260 258 242 179 150
99 97 23
NaeI GCC'GGC
Cuts at: 0 2985 3216
Size: 2985 231
NcoI C'CATG_G
Cuts at: 0 732 3216
Size: 732 2484
NdeI CA'TA_TG
Cuts at: 0 1114 3216
Size: 1114 2102
NgoMIV G'CCGG_C
Cuts at: 0 2983 3216
Size: 2983 233
NheI G'CTAG_C
Cuts at: 0 129 3216
Size: 129 3087
NruI TCG'CGA
Cuts at: 0 2309 3216
Size: 2309 907
NspI r_CATG'y
Cuts at: 0 1330 2658 3216
Size: 1330 1328 558
Fragments arranged by size:
1330 1328 558
PasI CC'CwG_GG
Cuts at: 0 858 1630 2571 3216
Size: 858 772 941 645
Fragments arranged by size:
941 858 772 645
PflMI CCAn_nnn'nTGG
Cuts at: 0 3042 3120 3216
Size: 3042 78 96
Fragments arranged by size:
3042 96 78
PfoI T'CCnGG_A
Cuts at: 0 233 1484 1592 3216
Size: 233 1251 108 1624
Fragments arranged by size:
1624 1251 233 108
PpuMI rG'GwC_Cy
Cuts at: 0 780 1012 1363 1626 2376 3216
Size: 780 232 351 263 750 840
Fragments arranged by size:
840 780 750 351 263 232
PspOMI G'GGCC_C
Cuts at: 0 1378 1820 2111 2879 3216
Size: 1378 442 291 768 337
Fragments arranged by size:
1378 768 442 337 291
PspXI vC'TCGA_Gb
Cuts at: 0 1266 3216
Size: 1266 1950
PstI C_TGCA'G
Cuts at: 0 149 563 788 1538 1565 2000 2695 2749
Size: 149 414 225 750 27 435 695 54
Cuts at: 2749 3216
Size: 467
Fragments arranged by size:
750 695 467 435 414 225 149 54
27
PvuII CAG'CTG
Cuts at: 0 150 528 1096 1964 2436 2875 2974 3216
Size: 150 378 568 868 472 439 99 242
Fragments arranged by size:
868 568 472 439 378 242 150 99
SacI G_AGCT'C
Cuts at: 0 479 1748 3216
Size: 479 1269 1468
Fragments arranged by size:
1468 1269 479
SalI G'TCGA_C
Cuts at: 0 840 3216
Size: 840 2376
SanDI GG'GwC_CC
Cuts at: 0 1012 1363 2376 3216
Size: 1012 351 1013 840
Fragments arranged by size:
1013 1012 840 351
SapI GCTCTTCn'nnn_
Cuts at: 0 3065 3216
Size: 3065 151
SbfI CC_TGCA'GG
Cuts at: 0 788 3216
Size: 788 2428
ScaI AGT'ACT
Cuts at: 0 458 1540 3216
Size: 458 1082 1676
Fragments arranged by size:
1676 1082 458
SexAI A'CCwGG_T
Cuts at: 0 431 3216
Size: 431 2785
SfcI C'TryA_G
Cuts at: 0 145 440 559 784 1534 1561 1834 1996
Size: 145 295 119 225 750 27 273 162
Cuts at: 1996 2691 2745 2836 3216
Size: 695 54 91 380
Fragments arranged by size:
750 695 380 295 273 225 162 145
119 91 54 27
SmaI CCC'GGG
Cuts at: 0 2884 3216
Size: 2884 332
SmlI C'TyrA_G
Cuts at: 0 1266 2780 3216
Size: 1266 1514 436
Fragments arranged by size:
1514 1266 436
SpeI A'CTAG_T
Cuts at: 0 1303 3216
Size: 1303 1913
SphI G_CATG'C
Cuts at: 0 2658 3216
Size: 2658 558
SrfI GCCC'GGGC
Cuts at: 0 2884 3216
Size: 2884 332
StuI AGG'CCT
Cuts at: 0 1031 1463 3216
Size: 1031 432 1753
Fragments arranged by size:
1753 1031 432
StyI C'CwwG_G
Cuts at: 0 732 943 1017 3185 3216
Size: 732 211 74 2168 31
Fragments arranged by size:
2168 732 211 74 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 755 1269 1627 2022 2371 3216
Size: 755 514 358 395 349 845
Fragments arranged by size:
845 755 514 395 358 349
TatI w'GTAC_w
Cuts at: 0 456 1538 3216
Size: 456 1082 1678
Fragments arranged by size:
1678 1082 456
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 152 184 608 627 640 659 673 705
Size: 152 32 424 19 13 19 14 32
Cuts at: 705 1796 1828 2148 2180 3216
Size: 1091 32 320 32 1036
Fragments arranged by size:
1091 1036 424 320 152 32 32 32
32 19 19 14 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 730 772 1152 2407 3121 3216
Size: 730 42 380 1255 714 95
Fragments arranged by size:
1255 730 714 380 95 42
XhoI C'TCGA_G
Cuts at: 0 1266 2780 3216
Size: 1266 1514 436
Fragments arranged by size:
1514 1266 436
XmaI C'CCGG_G
Cuts at: 0 2882 3216
Size: 2882 334
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HindIII KpnI MmeI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NruI NspI PasI PflMI
PfoI PpuMI PspOMI PspXI PstI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AscI AseI
AsiSI AvrII BbeI BclI BfrBI BglI BmgBI BpuEI
BsaBI BsmI BspEI BspHI BsrGI BssHII BssSI BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII EcoRI EcoRV
FseI FspI FspAI HpaI KasI MfeI MluI MscI
NarI NotI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SacII SfiI SfoI
SgfI SgrAI SnaBI SspI SwaI Tth111I XbaI XmnI
ZraI