Restriction map for
pF1KB7014
(Linear) (Six-base) MAPSORT of: KIBB7014.seq Check: 3165 from: 1 to: 5742
KIBB7014.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 69 1560 2604 3597 3712 3744 5703 5742
Size: 69 1491 1044 993 115 32 1959 39
Fragments arranged by size:
1959 1491 1044 993 115 69 39 32
AatII G_ACGT'C
Cuts at: 0 4772 4870 5742
Size: 4772 98 872
Fragments arranged by size:
4772 872 98
AccI GT'mk_AC
Cuts at: 0 821 5742
Size: 821 4921
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3562 5142 5742
Size: 3562 1580 600
Fragments arranged by size:
3562 1580 600
AfeI AGC'GCT
Cuts at: 0 1717 2917 3123 5742
Size: 1717 1200 206 2619
Fragments arranged by size:
2619 1717 1200 206
AflIII A'CryG_T
Cuts at: 0 695 1607 2105 2156 5204 5742
Size: 695 912 498 51 3048 538
Fragments arranged by size:
3048 912 695 538 498 51
AhdI GACnn_n'nnGTC
Cuts at: 0 759 5742
Size: 759 4983
AleI CACnn'nnGTG
Cuts at: 0 2926 4270 5742
Size: 2926 1344 1472
Fragments arranged by size:
2926 1472 1344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4771 5302 5742
Size: 4771 531 440
Fragments arranged by size:
4771 531 440
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 3278 3310 4420 4452 5742
Size: 1048 32 2198 32 1110 32 1290
Fragments arranged by size:
2198 1290 1110 1048 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 197 474 1296 1673 2181 2633 4137 5694
Size: 197 277 822 377 508 452 1504 1557
Cuts at: 5694 5742
Size: 48
Fragments arranged by size:
1557 1504 822 508 452 377 277 197
48
ApaI G_GGCC'C
Cuts at: 0 303 316 2677 4480 5742
Size: 303 13 2361 1803 1262
Fragments arranged by size:
2361 1803 1262 303 13
ApaLI G'TGCA_C
Cuts at: 0 2109 5742
Size: 2109 3633
ApoI r'AATT_y
Cuts at: 0 925 2358 5742
Size: 925 1433 3384
Fragments arranged by size:
3384 1433 925
AvaI C'yCGr_G
Cuts at: 0 1919 2298 2665 2676 3103 3217 5742
Size: 1919 379 367 11 427 114 2525
Fragments arranged by size:
2525 1919 427 379 367 114 11
BamHI G'GATC_C
Cuts at: 0 1895 5742
Size: 1895 3847
BanI G'GyrC_C
Cuts at: 0 1581 1929 2980 3089 3262 3681 4000 4605
Size: 1581 348 1051 109 173 419 319 605
Cuts at: 4605 4727 4854 5002 5015 5742
Size: 122 127 148 13 727
Fragments arranged by size:
1581 1051 727 605 419 348 319 173
148 127 122 109 13
BanII G_rGCy'C
Cuts at: 0 303 316 1712 2677 2956 3115 4480 5179
Size: 303 13 1396 965 279 159 1365 699
Cuts at: 5179 5742
Size: 563
Fragments arranged by size:
1396 1365 965 699 563 303 279 159
13
BbeI G_GCGC'C
Cuts at: 0 1933 3685 4731 5742
Size: 1933 1752 1046 1011
Fragments arranged by size:
1933 1752 1046 1011
BbsI GAAGACnn'nnnn_
Cuts at: 0 2016 2741 5742
Size: 2016 725 3001
Fragments arranged by size:
3001 2016 725
BbvCI CC'TCA_GC
Cuts at: 0 131 1046 1528 4912 5742
Size: 131 915 482 3384 830
Fragments arranged by size:
3384 915 830 482 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 606 640 670 704 2561 2578 2595 2612
Size: 606 34 30 34 1857 17 17 17
Cuts at: 2612 5742
Size: 3130
Fragments arranged by size:
3130 1857 606 34 34 30 17 17
17
BclI T'GATC_A
Cuts at: 0 4979 5312 5742
Size: 4979 333 430
Fragments arranged by size:
4979 430 333
BglI GCCn_nnn'nGGC
Cuts at: 0 371 1013 3527 5742
Size: 371 642 2514 2215
Fragments arranged by size:
2514 2215 642 371
BglII A'GATC_T
Cuts at: 0 2195 5084 5615 5742
Size: 2195 2889 531 127
Fragments arranged by size:
2889 2195 531 127
BlpI GC'TnA_GC
Cuts at: 0 5178 5561 5742
Size: 5178 383 181
Fragments arranged by size:
5178 383 181
Bme1580I G_kGCm'C
Cuts at: 0 303 316 619 1023 1306 2113 2482 2677
Size: 303 13 303 404 283 807 369 195
Cuts at: 2677 2850 2983 4480 4685 4859 5119 5742
Size: 173 133 1497 205 174 260 623
Fragments arranged by size:
1497 807 623 404 369 303 303 283
260 205 195 174 173 133 13
BmgBI CAC'GTC
Cuts at: 0 306 890 1059 1610 2739 5259 5742
Size: 306 584 169 551 1129 2520 483
Fragments arranged by size:
2520 1129 584 551 483 306 169
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 508 3704 5742
Size: 207 301 3196 2038
Fragments arranged by size:
3196 2038 301 207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5637 5742
Size: 5637 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1903 2161 2325 3087 3529 3556 3886 3937
Size: 1903 258 164 762 442 27 330 51
Cuts at: 3937 4198 4333 4741 5014 5683 5728 5742
Size: 261 135 408 273 669 45 14
Fragments arranged by size:
1903 762 669 442 408 330 273 261
258 164 135 51 45 27 14
Bpu10I CC'TnA_GC
Cuts at: 0 131 1003 1046 1138 1528 4912 5581 5734
Size: 131 872 43 92 390 3384 669 153
Cuts at: 5734 5742
Size: 8
Fragments arranged by size:
3384 872 669 390 153 131 92 43
8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 2497 2638 3550 3871 4063 4261 4396
Size: 724 1773 141 912 321 192 198 135
Cuts at: 4396 5742
Size: 1346
Fragments arranged by size:
1773 1346 912 724 321 198 192 141
135
BsaI GGTCTCn'nnnn_
Cuts at: 0 1511 1946 3820 5082 5143 5742
Size: 1511 435 1874 1262 61 599
Fragments arranged by size:
1874 1511 1262 599 435 61
BsaAI yAC'GTr
Cuts at: 0 2106 2202 5742
Size: 2106 96 3540
Fragments arranged by size:
3540 2106 96
BsaBI GATnn'nnATC
Cuts at: 0 2992 5742
Size: 2992 2750
BsaHI Gr'CG_yC
Cuts at: 0 1324 1688 1838 1930 2831 2855 3682 4487
Size: 1324 364 150 92 901 24 827 805
Cuts at: 4487 4728 4769 4867 5363 5742
Size: 241 41 98 496 379
Fragments arranged by size:
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150 98 92 41 24
BsaWI w'CCGG_w
Cuts at: 0 3086 4025 5742
Size: 3086 939 1717
Fragments arranged by size:
3086 1717 939
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2776 2806 2821 2851 3439 3463 3469 3493
Size: 2776 30 15 30 588 24 6 24
Cuts at: 3493 4420 4450 4867 4897 5389 5419 5425 5455
Size: 927 30 417 30 492 30 6 30
Cuts at: 5455 5742
Size: 287
Fragments arranged by size:
2776 927 588 492 417 287 30 30
30 30 30 30 24 24 15 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 4881 5742
Size: 697 4184 861
Fragments arranged by size:
4184 861 697
BseYI C'CCAG_C
Cuts at: 0 12 44 349 1035 1230 1712 3152 3408
Size: 12 32 305 686 195 482 1440 256
Cuts at: 3408 3429 3547 4423 4691 5424 5486 5742
Size: 21 118 876 268 733 62 256
Fragments arranged by size:
1440 876 733 686 482 305 268 256
256 195 118 62 32 21 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 562 1009 1621 1648 1765 1777 2619
Size: 376 186 447 612 27 117 12 842
Cuts at: 2619 2793 3575 4114 5560 5742
Size: 174 782 539 1446 182
Fragments arranged by size:
1446 842 782 612 539 447 376 186
182 174 117 27 12
BsiEI CG_ry'CG
Cuts at: 0 1017 3041 3421 4209 5742
Size: 1017 2024 380 788 1533
Fragments arranged by size:
2024 1533 1017 788 380
BsiHKAI G_wGCw'C
Cuts at: 0 29 86 527 572 881 2113 3470 3626
Size: 29 57 441 45 309 1232 1357 156
Cuts at: 3626 4049 4079 4277 5420 5742
Size: 423 30 198 1143 322
Fragments arranged by size:
1357 1232 1143 441 423 322 309 198
156 57 45 30 29
BsiWI C'GTAC_G
Cuts at: 0 935 5742
Size: 935 4807
BsmI GAATG_Cn'
Cuts at: 0 3943 4599 5742
Size: 3943 656 1143
Fragments arranged by size:
3943 1143 656
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2662 3655 5742
Size: 2662 993 2087
Fragments arranged by size:
2662 2087 993
Bsp1286I G_dGCh'C
Cuts at: 0 29 86 303 316 527 572 619 881
Size: 29 57 217 13 211 45 47 262
Cuts at: 881 1023 1306 1712 2113 2482 2677 2850 2956
Size: 142 283 406 401 369 195 173 106
Cuts at: 2956 2983 3115 3470 3626 4049 4079 4277 4480
Size: 27 132 355 156 423 30 198 203
Cuts at: 4480 4685 4859 5119 5179 5420 5742
Size: 205 174 260 60 241 322
Fragments arranged by size:
423 406 401 369 355 322 283 262
260 241 217 211 205 203 198 195
174 173 156 142 132 106 60 57
47 45 30 29 27 13
BspHI T'CATG_A
Cuts at: 0 4232 5742
Size: 4232 1510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 69 1560 1739 2255 2405 2604 3597 3712
Size: 69 1491 179 516 150 199 993 115
Cuts at: 3712 3744 5703 5742
Size: 32 1959 39
Fragments arranged by size:
1959 1491 993 516 199 179 150 115
69 39 32
BsrBI CCG'CTC
Cuts at: 0 1907 3417 5000 5742
Size: 1907 1510 1583 742
Fragments arranged by size:
1907 1583 1510 742
BsrDI GCAATG_nn'
Cuts at: 0 572 1773 5742
Size: 572 1201 3969
Fragments arranged by size:
3969 1201 572
BsrFI r'CCGG_y
Cuts at: 0 871 1007 1402 1460 3047 4003 4608 5742
Size: 871 136 395 58 1587 956 605 1134
Fragments arranged by size:
1587 1134 956 871 605 395 136 58
BsrGI T'GTAC_A
Cuts at: 0 794 3341 5742
Size: 794 2547 2401
Fragments arranged by size:
2547 2401 794
BssHII G'CGCG_C
Cuts at: 0 4744 5742
Size: 4744 998
BssSI C'ACGA_G
Cuts at: 0 3866 3976 5611 5742
Size: 3866 110 1635 131
Fragments arranged by size:
3866 1635 131 110
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1743 2633 4155 5694 5742
Size: 1743 890 1522 1539 48
Fragments arranged by size:
1743 1539 1522 890 48
BstBI TT'CG_AA
Cuts at: 0 2732 5742
Size: 2732 3010
BstEII G'GTnAC_C
Cuts at: 0 4635 5056 5742
Size: 4635 421 686
Fragments arranged by size:
4635 686 421
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2232 3993 5742
Size: 2232 1761 1749
Fragments arranged by size:
2232 1761 1749
BstYI r'GATC_y
Cuts at: 0 743 1430 1895 2177 2195 2648 3083 5084
Size: 743 687 465 282 18 453 435 2001
Cuts at: 5084 5243 5615 5648 5742
Size: 159 372 33 94
Fragments arranged by size:
2001 743 687 465 453 435 372 282
159 94 33 18
Bsu36I CC'TnA_GG
Cuts at: 0 3167 3205 4601 5742
Size: 3167 38 1396 1141
Fragments arranged by size:
3167 1396 1141 38
BtgI C'CryG_G
Cuts at: 0 67 369 1185 1210 1361 2961 3356 4209
Size: 67 302 816 25 151 1600 395 853
Cuts at: 4209 4706 5061 5742
Size: 497 355 681
Fragments arranged by size:
1600 853 816 681 497 395 355 302
151 67 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5496 5742
Size: 5496 246
BtsI GCAGTG_nn'
Cuts at: 0 260 289 1744 1899 2356 2577 3027 3748
Size: 260 29 1455 155 457 221 450 721
Cuts at: 3748 5119 5742
Size: 1371 623
Fragments arranged by size:
1455 1371 721 623 457 450 260 221
155 29
ClaI AT'CG_AT
Cuts at: 0 626 1259 5742
Size: 626 633 4483
Fragments arranged by size:
4483 633 626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3219 3254 4764 4799 5742
Size: 3219 35 1510 35 943
Fragments arranged by size:
3219 1510 943 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 216 2121 4074 5022 5742
Size: 216 1905 1953 948 720
Fragments arranged by size:
1953 1905 948 720 216
DrdI GACnn_nn'nnGTC
Cuts at: 0 4768 4836 5742
Size: 4768 68 906
Fragments arranged by size:
4768 906 68
EaeI y'GGCC_r
Cuts at: 0 188 674 1014 1416 1458 1890 2234 2549
Size: 188 486 340 402 42 432 344 315
Cuts at: 2549 3045 3272 3380 3418 4940 5105 5192 5742
Size: 496 227 108 38 1522 165 87 550
Fragments arranged by size:
1522 550 496 486 432 402 344 340
315 227 188 165 108 87 42 38
EagI C'GGCC_G
Cuts at: 0 1014 3418 5742
Size: 1014 2404 2324
Fragments arranged by size:
2404 2324 1014
EarI CTCTTCn'nnn_
Cuts at: 0 496 613 2293 4792 5269 5742
Size: 496 117 1680 2499 477 473
Fragments arranged by size:
2499 1680 496 477 473 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1905 3818 4860 5742
Size: 1905 1913 1042 882
Fragments arranged by size:
1913 1905 1042 882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1903 2161 2325 3087 3529 3556 3562 3886
Size: 1903 258 164 762 442 27 6 324
Cuts at: 3886 3937 4198 4333 4741 5014 5142 5683 5728
Size: 51 261 135 408 273 128 541 45
Cuts at: 5728 5742
Size: 14
Fragments arranged by size:
1903 762 541 442 408 324 273 261
258 164 135 128 51 45 27 14
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 988 1045 2863 2972 4519 5665 5742
Size: 988 57 1818 109 1547 1146 77
Fragments arranged by size:
1818 1547 1146 988 109 77 57
EcoO109I rG'GnC_Cy
Cuts at: 0 41 201 313 1638 1791 3192 3242 4476
Size: 41 160 112 1325 153 1401 50 1234
Cuts at: 4476 4477 4838 5742
Size: 1 361 904
Fragments arranged by size:
1401 1325 1234 904 361 160 153 112
50 41 1
EcoRI G'AATT_C
Cuts at: 0 2358 5742
Size: 2358 3384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3966 4167 5742
Size: 3966 201 1575
Fragments arranged by size:
3966 1575 201
FseI GG_CCGG'CC
Cuts at: 0 1464 3051 5742
Size: 1464 1587 2691
Fragments arranged by size:
2691 1587 1464
FspI TGC'GCA
Cuts at: 0 3394 4936 5742
Size: 3394 1542 806
Fragments arranged by size:
3394 1542 806
HaeII r_GCGC'y
Cuts at: 0 452 1719 1933 2559 2919 3125 3685 4731
Size: 452 1267 214 626 360 206 560 1046
Cuts at: 4731 5606 5742
Size: 875 136
Fragments arranged by size:
1267 1046 875 626 560 452 360 214
206 136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 712 744 745 777 1508 1540
Size: 619 32 61 32 1 32 731 32
Cuts at: 1540 2821 2853 4825 4857 5389 5421 5425 5457
Size: 1281 32 1972 32 532 32 4 32
Cuts at: 5457 5538 5570 5742
Size: 81 32 172
Fragments arranged by size:
1972 1281 731 619 532 172 81 61
32 32 32 32 32 32 32 32
32 4 1
HincII GTy'rAC
Cuts at: 0 396 1842 2067 2154 5742
Size: 396 1446 225 87 3588
Fragments arranged by size:
3588 1446 396 225 87
KasI G'GCGC_C
Cuts at: 0 1929 3681 4727 5742
Size: 1929 1752 1046 1015
Fragments arranged by size:
1929 1752 1046 1015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3638 4316 4910 5468 5742
Size: 3638 678 594 558 274
Fragments arranged by size:
3638 678 594 558 274
MscI TGG'CCA
Cuts at: 0 676 3274 3382 5107 5194 5742
Size: 676 2598 108 1725 87 548
Fragments arranged by size:
2598 1725 676 548 108 87
MslI CAynn'nnrTG
Cuts at: 0 2926 3355 4042 4270 4975 5742
Size: 2926 429 687 228 705 767
Fragments arranged by size:
2926 767 705 687 429 228
MspA1I CmG'CkG
Cuts at: 0 60 69 852 1035 1749 1834 1959 2561
Size: 60 9 783 183 714 85 125 602
Cuts at: 2561 3236 4134 4211 4284 4434 4695 4708 5063
Size: 675 898 77 73 150 261 13 355
Cuts at: 5063 5190 5691 5742
Size: 127 501 51
Fragments arranged by size:
898 783 714 675 602 501 355 261
183 150 127 125 85 77 73 60
51 13 9
NaeI GCC'GGC
Cuts at: 0 873 1009 1404 1462 3049 4005 5742
Size: 873 136 395 58 1587 956 1737
Fragments arranged by size:
1737 1587 956 873 395 136 58
NarI GG'CG_CC
Cuts at: 0 1930 3682 4728 5742
Size: 1930 1752 1046 1014
Fragments arranged by size:
1930 1752 1046 1014
NcoI C'CATG_G
Cuts at: 0 3356 5742
Size: 3356 2386
NdeI CA'TA_TG
Cuts at: 0 4032 5742
Size: 4032 1710
NgoMIV G'CCGG_C
Cuts at: 0 871 1007 1402 1460 3047 4003 5742
Size: 871 136 395 58 1587 956 1739
Fragments arranged by size:
1739 1587 956 871 395 136 58
NspI r_CATG'y
Cuts at: 0 126 699 4932 5208 5742
Size: 126 573 4233 276 534
Fragments arranged by size:
4233 573 534 276 126
PasI CC'CwG_GG
Cuts at: 0 146 456 1080 1296 5742
Size: 146 310 624 216 4446
Fragments arranged by size:
4446 624 310 216 146
PciI A'CATG_T
Cuts at: 0 695 5204 5742
Size: 695 4509 538
Fragments arranged by size:
4509 695 538
PflMI CCAn_nnn'nTGG
Cuts at: 0 197 216 375 1035 1296 1962 5012 5742
Size: 197 19 159 660 261 666 3050 730
Fragments arranged by size:
3050 730 666 660 261 197 159 19
PfoI T'CCnGG_A
Cuts at: 0 1880 2138 3064 5216 5742
Size: 1880 258 926 2152 526
Fragments arranged by size:
2152 1880 926 526 258
PmlI CAC'GTG
Cuts at: 0 2106 5742
Size: 2106 3636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4420 4452 5742
Size: 4420 32 1290
Fragments arranged by size:
4420 1290 32
PpuMI rG'GwC_Cy
Cuts at: 0 1638 1791 3192 3242 4838 5742
Size: 1638 153 1401 50 1596 904
Fragments arranged by size:
1638 1596 1401 904 153 50
PshAI GACnn'nnGTC
Cuts at: 0 2741 5742
Size: 2741 3001
PspOMI G'GGCC_C
Cuts at: 0 299 312 2673 4476 5742
Size: 299 13 2361 1803 1266
Fragments arranged by size:
2361 1803 1266 299 13
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 928 960 5742
Size: 928 32 4782
Fragments arranged by size:
4782 928 32
PstI C_TGCA'G
Cuts at: 0 80 839 1256 1739 1808 2038 2351 3755
Size: 80 759 417 483 69 230 313 1404
Cuts at: 3755 3899 3965 4247 4760 5126 5742
Size: 144 66 282 513 366 616
Fragments arranged by size:
1404 759 616 513 483 417 366 313
282 230 144 80 69 66
PvuII CAG'CTG
Cuts at: 0 852 1035 1749 1834 4134 4284 5742
Size: 852 183 714 85 2300 150 1458
Fragments arranged by size:
2300 1458 852 714 183 150 85
SacII CC_GC'GG
Cuts at: 0 70 4212 4709 5064 5742
Size: 70 4142 497 355 678
Fragments arranged by size:
4142 678 497 355 70
SanDI GG'GwC_CC
Cuts at: 0 1638 3242 5742
Size: 1638 1604 2500
Fragments arranged by size:
2500 1638 1604
SapI GCTCTTCn'nnn_
Cuts at: 0 613 5269 5742
Size: 613 4656 473
Fragments arranged by size:
4656 613 473
SbfI CC_TGCA'GG
Cuts at: 0 80 4247 4760 5742
Size: 80 4167 513 982
Fragments arranged by size:
4167 982 513 80
ScaI AGT'ACT
Cuts at: 0 384 643 5742
Size: 384 259 5099
Fragments arranged by size:
5099 384 259
SexAI A'CCwGG_T
Cuts at: 0 4823 5018 5742
Size: 4823 195 724
Fragments arranged by size:
4823 724 195
SfcI C'TryA_G
Cuts at: 0 76 835 1252 1735 1804 2034 2347 3751
Size: 76 759 417 483 69 230 313 1404
Cuts at: 3751 3895 3961 4243 4756 5122 5742
Size: 144 66 282 513 366 620
Fragments arranged by size:
1404 759 620 513 483 417 366 313
282 230 144 76 69 66
SfoI GGC'GCC
Cuts at: 0 1931 3683 4729 5742
Size: 1931 1752 1046 1013
Fragments arranged by size:
1931 1752 1046 1013
SgrAI Cr'CCGG_yG
Cuts at: 0 871 5742
Size: 871 4871
SmaI CCC'GGG
Cuts at: 0 1921 2300 3105 5742
Size: 1921 379 805 2637
Fragments arranged by size:
2637 1921 805 379
SmlI C'TyrA_G
Cuts at: 0 739 2512 2653 3565 3886 4078 4276 4411
Size: 739 1773 141 912 321 192 198 135
Cuts at: 4411 5742
Size: 1331
Fragments arranged by size:
1773 1331 912 739 321 198 192 141
135
SphI G_CATG'C
Cuts at: 0 4932 5742
Size: 4932 810
StuI AGG'CCT
Cuts at: 0 1810 5742
Size: 1810 3932
StyI C'CwwG_G
Cuts at: 0 37 1985 3356 4646 4700 5111 5462 5742
Size: 37 1948 1371 1290 54 411 351 280
Fragments arranged by size:
1948 1371 1290 411 351 280 54 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 223 490 636 1576 2925 3027 4654 5292
Size: 223 267 146 940 1349 102 1627 638
Cuts at: 5292 5478 5742
Size: 186 264
Fragments arranged by size:
1627 1349 940 638 267 264 223 186
146 102
TatI w'GTAC_w
Cuts at: 0 382 641 794 1604 2792 3176 3341 3758
Size: 382 259 153 810 1188 384 165 417
Cuts at: 3758 4445 5201 5636 5742
Size: 687 756 435 106
Fragments arranged by size:
1188 810 756 687 435 417 384 382
259 165 153 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 517 549 2441 2473 2531 2563 3063 3095
Size: 517 32 1892 32 58 32 500 32
Cuts at: 3095 4708 4740 5742
Size: 1613 32 1002
Fragments arranged by size:
1892 1613 1002 517 500 58 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 391 1522 2020 4353 4633 5742
Size: 391 1131 498 2333 280 1109
Fragments arranged by size:
2333 1131 1109 498 391 280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 609 3531 5742
Size: 609 2922 2211
Fragments arranged by size:
2922 2211 609
XmaI C'CCGG_G
Cuts at: 0 1919 2298 3103 5742
Size: 1919 379 805 2639
Fragments arranged by size:
2639 1919 805 379
XmnI GAAnn'nnTTC
Cuts at: 0 4150 5742
Size: 4150 1592
ZraI GAC'GTC
Cuts at: 0 4770 4868 5742
Size: 4770 98 874
Fragments arranged by size:
4770 874 98
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuII SacII SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgrAI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AscI AseI AsiSI AvrII
BaeI BciVI BfrBI BmtI BspEI BstZ17I DraI EcoICRI
EcoRV FspAI HindIII HpaI KpnI MfeI MluI NheI
NotI NruI NsiI PacI PmeI PsiI PspXI PvuI
RsrII SacI SalI SfiI SgfI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI